Novel Observations in 11 Heteroresistant Vancomycin-Intermediate Methicillin-Resistant Staphylococcus aureus Strains from South India

Bakthavatchalam, Yamuna Devi; Veeraraghavan, Balaji; Peter, John Victor; Rajinikanth, J.; Inbanathan, Francis Yesurajan; Ragupathi, Naveen Kumar Devanga; Sekar, Suresh Kumar Rajamani

doi:10.1128/genomea.01425-16

Cited by 7 publications

(5 citation statements)

References 7 publications

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“…Studies reported 5.8-6.9 per cent hVISA prevalence and most of the isolates showed vancomycin minimum inhibitory concentration (MIC) of ≥1.5 μg/ml1314. Our group has reported hVISA (n=17) harbouring multiple chromosomal mutations in teicoplanin-resistant operon ( tca RAB), vancomycin resistance associated ( vra SR), glycopeptide resistance-associated ( graSR ), cell wall regulating phosphorylase ( walKR ) two component systems (TCS) and rpo B gene1516. In addition, 10 VISA isolates with the vancomycin MIC of 4 μg/ml by microbroth dilution method was reported17.…”

Section: Gram-positive Organismsmentioning

confidence: 99%

Antimicrobial susceptibility profile & resistance mechanisms of Global Antimicrobial Resistance Surveillance System (GLASS) priority pathogens from India

Veeraraghavan¹,

Walia²

2019

Indian J Med Res

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Antimicrobial resistance is a major concern globally. Infections due to drug-resistant pathogens are becoming difficult and a challenge to treat. As treatment choices are limited due to the high-drug resistance rates, there is an increase in the health care cost, duration of hospital stay, morbidity and mortality rates. Understanding the true burden of antimicrobial resistance for a geographical location is important to guide effective empirical therapy. To have a national data, it is imperative to have a systemic data capturing across the country through surveillance studies. Very few surveillance studies have been conducted in India to generate national data on antimicrobial resistance. This review aims to report the cumulative antibiogram and the resistance mechanisms of Global Antimicrobial Resistance Surveillance System (GLASS) priority pathogens from India.

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Section: Gram-positive Organismsmentioning

confidence: 99%

Antimicrobial susceptibility profile & resistance mechanisms of Global Antimicrobial Resistance Surveillance System (GLASS) priority pathogens from India

Veeraraghavan¹,

Walia²

2019

Indian J Med Res

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“…Furthermore, both isolates carried the IEC system genes (ascribed to type E), which indicates a human origin since the adaptation of human strains to animals required genome alterations including the loss of elements that contain the IEC [31]. S. aureus ST1290-t131 was first reported to be associated with community-acquired MRSA and it has also been reported among vancomycin-intermediate S. aureus from bloodstream infection and wild rodents from Portugal [32][33][34]. Most S. aureus isolates from handlers were ascribed to ST30 and agr III which, in turn, belonged to four different spa-types (t021, t338, t012 and t1642).…”

Section: Discussionmentioning

confidence: 98%

A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

et al. 2022

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Donkeys (Equus asinus) are in decline in Europe. Occupational exposure to farm animals has been associated with increased staphylococci carriage. We aimed to isolate S. aureus and coagulase-negative staphylococci (CoNS) from donkeys and handlers and characterize the antimicrobial resistance profiles and genetic lineages of S. aureus strains. Oral and nasal swab samples were collected from 49 Miranda donkeys and 23 handlers from 15 different farms. Staphylococci species were identified by MALDI-TOF MS. The presence of antimicrobial resistance genes and virulence factors was investigated by PCR. Molecular typing was performed in S. aureus isolates. From the 49 donkey samples, 4 S. aureus (8.2%) and 21 CoNS (42.9%) were isolated. Ten handlers (43.5%) were carriers of S. aureus and 4 (17.4%) carried CoNS. The CoNS isolates showed resistance to several classes of antimicrobials encoded by the mecA, aph (3′)-IIIa, ant (4′)-Ia, tetM, tetK, lnuA, ermB, ermC, dfrA and dfrG genes. S. aureus isolates were resistant to penicillin, aminoglicosides and tetracycline harboring the blaZ, aph (3′)-IIIa, tetL, tetM and tetK genes. All S. aureus isolates from donkeys belonged to ST49 and spa-type t208 while the strains isolated from the handlers were ascribed to 3 STs and 7 spa-types. However, human isolates were from different STs than the donkey isolates. Donkeys are mainly colonized by methicillin-resistant S. sciuri. S. aureus transmission between donkeys and their handlers appears not to have occurred since the isolates belonged to different genetic lineages.

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“…[13,14] Only a few studies from India have previously reported reduced susceptibility to vancomycin after the introduction of the revised CLSI guidelines. [15][16][17][18][19] Hence, we aimed to determine the recent pattern of antibiotic resistance, assess the vancomycin susceptibility profile, and ascertain other therapeutic options for clinical S. aureus isolates in Eastern India, which would help in choosing empirical antimicrobial therapy as well as in administering targeted therapy to the patients.…”

Section: Introductionmentioning

confidence: 99%

Recent pattern of antibiotic resistance in Staphylococcus aureus clinical isolates in Eastern India and the emergence of reduced susceptibility to vancomycin

et al. 2019

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PURPOSE: We aimed to determine the recent pattern of antibiotic resistance and assess the vancomycin susceptibility profile of clinical Staphylococcus aureus in view of emerging reports of vancomycin creep, reduced vancomycin susceptibility (RVS), including heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus, and vancomycin resistance in S. aureus isolates. MATERIALS AND METHODS: Consecutive, nonduplicate isolates of S. aureus between July 2015 and June 2016 were subjected to antimicrobial susceptibility testing using standard disk diffusion test or Etest as per the Clinical and Laboratory Standards Institute 2015. Detection of hVISA was done by glycopeptide resistance detection Etest according to the manufacturer's instructions in strains with vancomycin minimum inhibitory concentration of 1–2 μg/ml. RESULTS: A total of 284 S. aureus were obtained from pus (175, 61.6%), respiratory tract (31, 10.9%), urine (27, 9.5%), blood (25, 8.8%), body fluids (18, 6.3%), and catheter tips (8, 2.8%). 127 (44.7%) isolates were methicillin resistant, and 158 (55.6%) were multidrug resistant. High resistance was observed to penicillin (81.7%), erythromycin (62.3%), and ciprofloxacin (52.1%), whereas the resistance was low to gentamicin (5.3%), rifampicin (8.1%), and doxycycline (9.5%). Two hundred and fifty-one (88.3%) isolates were fully susceptible to vancomycin, whereas 33 (11.6%) demonstrated RVS. All were uniformly susceptible to linezolid, tigecycline, and daptomycin. CONCLUSIONS: A moderately high percentage of S. aureus isolates demonstrated RVS, which may limit its usefulness in methicillin-resistant isolates and may be associated with increased complications in methicillin-susceptible infections. In view of increasing glycopeptide resistance, the susceptibility status of vancomycin along with other antibiotics among clinical S. aureus isolates should be investigated periodically.

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Novel Observations in 11 Heteroresistant Vancomycin-Intermediate Methicillin-Resistant Staphylococcus aureus Strains from South India

Cited by 7 publications

References 7 publications

Antimicrobial susceptibility profile & resistance mechanisms of Global Antimicrobial Resistance Surveillance System (GLASS) priority pathogens from India

Antimicrobial susceptibility profile & resistance mechanisms of Global Antimicrobial Resistance Surveillance System (GLASS) priority pathogens from India

A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

Recent pattern of antibiotic resistance in Staphylococcus aureus clinical isolates in Eastern India and the emergence of reduced susceptibility to vancomycin

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