Novel nanocomposite scaffold based on gelatin/PLGA-PEG-PLGA hydrogels embedded with TGF-β1 for chondrogenic differentiation of human dental pulp stem cells in vitro

Ghandforoushan, Parisa; Hanaee, Jalal; Aghazadeh, Zahra; Samiei, Mohammad; Navali, Amir Mohammad; Khatibi, Ali; Davaran, Soodabeh

doi:10.1016/j.ijbiomac.2021.12.097

Cited by 26 publications

(19 citation statements)

References 86 publications

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“…That may explained why the PHPB-G group promoted bone formation more efficiently in diabetic SD rats. The biological scaffolds can carry a variety of biological factors, including TGF- β , BMP2, and cellular exosomes [ 32 – 34 ]. However, the true bone remodeling environment in diabetic patients may be much more complex [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel Glucose-Sensitive Scaffold Accelerates Osteogenesis in Diabetic Conditions

Jiang

Bao

2022

BioMed Research International

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Mandibular bone regeneration is still a big challenge in those diabetic patients with poorly controlled blood glucose. In this study, we prepared a novel glucose-sensitive controlled-release fiber scaffold (PVA-HTCC/PEO-rhBMP2-glucose oxidase (PHPB-G)), which contained the recombinant human bone morphogenetic protein 2 (rhBMP2) by coaxial cospinning and grafted with glucose oxidase (GOD). We presented evidence that PHPB-G could undergo a series of structural changes with the blood glucose and promoted bone regeneration in diabetic rat. PHPB-G expanded the voids in nanofibers when blood glucose levels elevated. More importantly, its slow-release rhBMP2 effectively promoted the healing of bone defects. These data suggested that the PHPB-G delivery system may provide a potential treatment strategy for patients with severe diabetic alveolar bone defects.

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Section: Discussionmentioning

confidence: 99%

A Novel Glucose-Sensitive Scaffold Accelerates Osteogenesis in Diabetic Conditions

Jiang

Bao

2022

BioMed Research International

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“…TGF-β1-Loaded PLGA-PEG-PLGA copolymer was constructed via the double emulsion method. All the synthesis details have been mentioned in our recently published paper [34].…”

Section: Methodsmentioning

confidence: 99%

Enhancing the function of PLGA-collagen scaffold by incorporating TGF-β1-loaded PLGA-PEG-PLGA nanoparticles for cartilage tissue engineering using human dental pulp stem cells

Ghandforoushan

Hanaee

Aghazadeh

et al. 2022

Drug Deliv. and Transl. Res.

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Researchers have scrutinized cartilage tissue regeneration to handle the de ciency of cartilage restoration capacity. This investigation proposed to compose an innovative nanocomposite biomaterial that enhances growth factor delivery to the injured cartilage site. Here, we describe the design and development of the biocompatible PLGA-collagen / PLGA-PEG-PLGA nanocomposite scaffold containing TGF-β1. PLGA-PEG-PLGA nanoparticles were employed as a delivery system embedding TGF-β1 as an articular cartilage repair therapeutic agent. This study evaluates various physicochemical aspects of fabricated scaffolds by 1 HNMR, FT-IR, SEM, BET, and DLS methods. The physicochemical features of the developed scaffolds, including porosity, density, degradation, swelling ratio, mechanical properties, morphologies, BET, ELIZA, and cytotoxicity were assessed. SEM images displayed suitable cell adhesion and distribution of hDPSCs throughout the scaffolds. The cell viability was investigated with the MTT test. In real-time PCR testing, the expression of Sox-9, collagen type II, and aggrecan genes was monitored. According to the results, h-DPSCs exhibited high adhesion, proliferation, and differentiation on PLGA-collagen/PLGA-PEG-PLGA-TGF-β1 hydrogels compared to the control groups. RT-PCR assay data displayed that TGF-β1 loaded PLGA-PEG-PLGA nanoparticles puts forward chondroblast differentiation in hDPSCs through the expression of chondrogenic genes. Due to its potential for the growth and differentiation of hDPSCc, PLGA-collagen/PLGA-PEG-PLGA-TGF-β1 hydrogel is likely to be a suitable biomaterial for cartilage regeneration.

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“…For instance, biodegradable and biocompatible hydrogels induce chondrogenic differentiation when TGF‐β1, a TGF superfamily polypeptide that acts as a cytokine, is incorporated 143 . This transforming growth factor enhances the cartilaginous ECM production in gelatin/PLGA‐PEG‐PLGA hydrogels, evidencing an increase in SOX9 and aggrecan 144 . It has been proved that the expression of Col2α1‐IIa and aggrecan through TGF‐β1 is thank to the activation of the Smad2/3 signaling pathway, which promotes the formation of a cartilage‐like ECM and the development of cartilage‐like tissue 145,146 .…”

Section: Injectable Hydrogels Used For Hyaline Cartilage Recoverymentioning

confidence: 99%

“…Stem cells could differentiate into multiple cell types, and this process is regulated by various signaling pathways 65,155 . Therefore, it is important to maintain the appropriate signaling pathways to promote chondrogenic differentiation while inhibiting other differentiation pathways 144 …”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Anatomy, molecular structures, and hyaluronic acid – Gelatin injectable hydrogels as a therapeutic alternative for hyaline cartilage recovery: A review

Vaca‐González,

Culma,

Nova

et al. 2023

J Biomed Mater Res

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Cartilage damage caused by trauma or osteoarthritis is a common joint disease that can increase the social and economic burden in society. Due to its avascular characteristics, the poor migration ability of chondrocytes, and a low number of progenitor cells, the self-healing ability of cartilage defects has been significantly limited. Hydrogels have been developed into one of the most suitable biomaterials for the regeneration of cartilage because of its characteristics such as high-water absorption, biodegradation, porosity, and biocompatibility similar to natural extracellular matrix.Therefore, the present review article presents a conceptual framework that summarizes the anatomical, molecular structure and biochemical properties of hyaline cartilage located in long bones: articular cartilage and growth plate. Moreover, the importance of preparation and application of hyaluronic acidgelatin hydrogels for cartilage tissue engineering are included. Hydrogels possess benefits of stimulating the production of Agc1, Col2α1-IIa, and SOX9, molecules important for the synthesis and composition of the extracellular matrix of cartilage. Accordingly, they are believed to be promising biomaterials of therapeutic alternatives to treat cartilage damage.

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Novel nanocomposite scaffold based on gelatin/PLGA-PEG-PLGA hydrogels embedded with TGF-β1 for chondrogenic differentiation of human dental pulp stem cells in vitro

Cited by 26 publications

References 86 publications

A Novel Glucose-Sensitive Scaffold Accelerates Osteogenesis in Diabetic Conditions

A Novel Glucose-Sensitive Scaffold Accelerates Osteogenesis in Diabetic Conditions

Enhancing the function of PLGA-collagen scaffold by incorporating TGF-β1-loaded PLGA-PEG-PLGA nanoparticles for cartilage tissue engineering using human dental pulp stem cells

Anatomy, molecular structures, and hyaluronic acid – Gelatin injectable hydrogels as a therapeutic alternative for hyaline cartilage recovery: A review

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