Enhancing the function of PLGA-collagen scaffold by incorporating TGF-β1-loaded PLGA-PEG-PLGA nanoparticles for cartilage tissue engineering using human dental pulp stem cells

Abstract

Researchers have scrutinized cartilage tissue regeneration to handle the de ciency of cartilage restoration capacity. This investigation proposed to compose an innovative nanocomposite biomaterial that enhances growth factor delivery to the injured cartilage site. Here, we describe the design and development of the biocompatible PLGA-collagen / PLGA-PEG-PLGA nanocomposite scaffold containing TGF-β1. PLGA-PEG-PLGA nanoparticles were employed as a delivery system embedding TGF-β1 as an articular cartilage repair therapeutic agent. This study evaluates various physicochemical aspects of fabricated scaffolds by 1 HNMR, FT-IR, SEM, BET, and DLS methods. The physicochemical features of the developed scaffolds, including porosity, density, degradation, swelling ratio, mechanical properties, morphologies, BET, ELIZA, and cytotoxicity were assessed. SEM images displayed suitable cell adhesion and distribution of hDPSCs throughout the scaffolds. The cell viability was investigated with the MTT test. In real-time PCR testing, the expression of Sox-9, collagen type II, and aggrecan genes was monitored. According to the results, h-DPSCs exhibited high adhesion, proliferation, and differentiation on PLGA-collagen/PLGA-PEG-PLGA-TGF-β1 hydrogels compared to the control groups. RT-PCR assay data displayed that TGF-β1 loaded PLGA-PEG-PLGA nanoparticles puts forward chondroblast differentiation in hDPSCs through the expression of chondrogenic genes. Due to its potential for the growth and differentiation of hDPSCc, PLGA-collagen/PLGA-PEG-PLGA-TGF-β1 hydrogel is likely to be a suitable biomaterial for cartilage regeneration.

Sustained Delivery of Methylsulfonylmethane from Biodegradable Scaffolds Enhances Efficient Bone Regeneration

“…Similar to this study, Ghandforoushan et al supplemented TGF-β1 into a PLGA-collagen scaffold with PLGA-poly (ethylene glycol)-PLGA nanoparticles as delivery vehicles to enhance the chondrogenic differentiation of hDPSCs. 64 In all, it can be expected that the tissue engineering strategy by combining 3D biomimetic structure and sustained release of therapeutic agents would represent technological advancements for tissue repair and clinical therapy.…”

Dental pulp regeneration strategies: A review of status quo and recent advances

“…PEG materials also can be used as a delivery system. For instance, Ghandforoushan et al created a composite scaffold by embedding TGF-β1-containing nanoparticles within PEG and PLGA, it regulated DPSCs adhesion, proliferation, and differentiation, ultimately promoting their chondrogenic differentiation [ 87 ].Therefore, the use of these polymeric materials may be considered an interesting strategy for regenerative pulp therapy.…”

Functional injectable hydrogel with spatiotemporal sequential release for recruitment of endogenous stem cells and in situ cartilage regeneration

“…Meanwhile, the KGN MPs filled the void spaces of the fibrous network and impeded the movement of the fibrous networks, thereby increasing the mechanical strength of the hydrogel. 46,47 Therefore, Col-Apt@KGN MPs could provide biological scaffolds with functionality and mechanical properties, which implied that the functional injectable hydrogel system was successfully fabricated.…”