Novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW)

Abstract

A novel method utilizing microbial treatment for cleaner production of diosgenin from Dioscorea zingiberensis C.H. Wright (DZW) was presented. A new Bacillus pumilus HR19, which has the great ability to secrete pectinase, was screened and applied in the microbial treatment. Low-pressure steam expansion pretreatment (LSEP) was employed in advance to assist microbial treatment efficiently in releasing saponins, which are the precursors of diosgenin. Compared with the traditional process of acid hydrolysis, this novel process reduced the consumptions of water, acid and organic solvent by more than 92.5%, 97.0%, 97.0%, respectively, while simultaneously increasing the diosgenin yield by 6.21%. In addition, the microbial treatment was more efficient than enzymatic treatment, which arised from that microorganisms could be induced to secrete related enzymes by the compositions of DZW and relieve product inhibition by utilizing enzyme hydrolysates.

Influence of Soil Variation on Diosgenin Content Profile in Costus speciosus from Indo‐Gangetic Plains

“…Literature suggests that diosgenin is synthesized in its glycosidic form through phenylpropanoid pathway in the leaves and then, translocated to other plant parts, highest concentration being in the rhizomes [22] . The glycosidic form of diosgenin is pharmacologically less active and thus, for the production of active molecule the glycosidic bond needs to be hydrolyzed through acids, [16] enzymes [23] and, microorganisms [22] …”

Biotransformation of steroidal saponins in sisal (Agave sisalanaPerrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China

“…[ 32 ] and Wei et al. [ 23 ] reported that a higher diosgenin yield by biotransformation could be achieved by optimizing various fermentation conditions. Therefore, further investigation needs to be carried out to provide higher tigogenin yield because of the complexity of enzymes during the biotransformation process.…”

Karakterisasi Kuantitatif Diosgenin dengan Spetrofotometri UV-Vis pada Koleksi Umbi Dioscorea spp. di Indonesia

“…Metode ekstraksi yang dipakai menggunakan campuran enzim dan ultrafiltrasi agar diperoleh kadar diosgenin optimal. Kelebihan metode tersebut dibanding metode konvensional ialah mengurangi penggunaan bahan kimia sehingga mengurangi pencemaran air (Liu et al 2010;Zhu et al 2010;Wei et al 2013;Pan et al 2014).…”