Biotransformation of steroidal saponins in sisal (Agave sisalanaPerrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China

Abstract

A rod-shaped bacterium was isolated from the soil in a karst area of Guilin, China and its biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin was presented for the first time. A total of 22 strains for the degradation of steroidal saponins in sisal were isolated from 48 soil samples, and the isolated rod-shaped, bacterial strain ZG-21 was used for the production of tigogenin due to its highest degradation efficiency of steroidal saponins in sisal. The parameters affecting biotransformation by strain ZG-21 were optimized. Under the optimized conditions of temperature (30 °C), pH (6), time (5 days) and substrate concentration (5 mg/mL), a maximum tigogenin yield of 26.7 mg/g was achieved. Compared with the conventional method of acid hydrolysis, the biotransformation method provided a clean and eco-friendly alternative for the production of tigogenin.

Comparative study of steroidal sapogenins content in leaves of five Agave species

“…In A. cupreata Trel., the hydrolysis yields were similar in all samples, regardless of the sampling locality. In general, the yields obtained in this work are good, although the hydrolysis conditions such as acid concentration, temperature and hydrolysis time can always be optimized, as reported for A. sisalana 19 …”

“…In general, the yields obtained in this work are good, although the hydrolysis conditions such as acid concentration, temperature and hydrolysis time can always be optimized, as reported for A. sisalana. 19 In GC-FID analysis of the hydrolysis products of A. cupreata Trel., A. americana var. oaxacencis L., A. angustifolia Haw., A. karwinskii Zucc.…”

Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

“…The trans-dihydrocarvone content increased first and then decreased with the increase of ethanol concentration, and it reached the maximum value when the concentration of ethanol was 0.8% (v/v). Temperature was an important factor for microbial growth and product yields by influencing enzyme activity, enzyme stability, substrate solubility and nutrient intake during the biotransformation process [43]. High or low temperatures will be harmful to the enzymatic systems of the bacteria, thereby affecting the biotransformation process [44].…”

POTENSI DAN PEMANFAATAN LIMBAH DEKORTIKASI TANAMAN SISAL (Agave sisalana) Potential Use of Waste Plant Decortication of Sisal(Agave sisalana)

“…Sisal tumbuh sepanjang tahun, dapat dipanen pada umur 2,5 tahun setelah tanam, dan produktif selama 4-6 tahun (Hutten, 2016). Sisal banyak dibudidayakan di daerah tropis dan semi-kering di seluruh dunia (Wang et al, 2014). Penggunaan serat sisal pada awalnya hanya digunakan sebagai bahan baku tali temali seperti digunakan tali pengikat daun tembakau di Madura dan karung goni untuk kemasan produk pertanian seperti gula dan kopra (Murianingrum and Setyo-Budi, 2020).…”