Novel infrared spectroscopic method for the determination of crystallinity of hydroxyapatite minerals

Pleshko, Nancy; Boskey, Adele L.; Mendelsohn, Richard

doi:10.1016/s0006-3495(91)82113-0

Cited by 345 publications

(291 citation statements)

References 18 publications

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“…The mineral content decrease observed in the present study is much steeper than could be explained by the difference in the relative density of peritubular dentin alone, supporting the distinct nature of mantle dentin. The width of the mantle dentin zone (based here on its distinct mineral properties relative to the rest of dentin) has been a subject of controversy in addition to debate about its relative mineral and matrix content [7,[25][26]. In our results, mantle dentin in bovine incisors was represented by an 80-100μm wide strip, in agreement with earlier data [5].…”

Section: Discussionsupporting

confidence: 90%

Maturational changes in dentin mineral properties

Verdelis

Lukashova

Wright

et al. 2007

Bone

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In this study the changes in properties of the maturing mantle and circumpulpal dentin were quantitatively analyzed. Sections from six fetal bovine undecalcified incisors were used. Regions of mantle and circumpulpal dentin of sequential maturation stages were identified on spectroscopic images acquired by Fourier Transform Infrared Imaging. Spectroscopic parameters corresponding to mineral properties at these stages were analyzed and reported as a function of distance from the cervix of the incisor, the latter representing tissue age. Mineral parameters were correlated with distance from the cervix. Values of these parameters in mantle and circumpulpal dentin were compared. A multi-phasic pattern of changes was found for all the parameters examined, with most of the alterations occurring in the initial maturation period. The patterns of temporal variation in mantle and circumpulpal dentin mineral properties show distinct developmental stages and were not identical for the two dentin compartments. The study showed that mineral maturation in dentin is not a linear process and that mantle dentin is developmentally distinct from circumpulpal dentin, presenting at certain stages different physicochemical events during the maturation of the tissue.

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Section: Discussionsupporting

confidence: 90%

Maturational changes in dentin mineral properties

Verdelis

Lukashova

Wright

et al. 2007

Bone

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“…These peculiarities of MgHA/Coll can be similarly observed in the FTIR spectrum of young bone; while they tend to disappear in mature bone and in highly crystalline synthetic apatite [28][29][30]. Correlation was noted between the fractional intensity of ~1050 cm -1 band related to PO 4 3-group and the crystal size of the apatite: a reduction of the apatitic crystal size induced an increase of the percentage area of this component [31,32]. All these features indicate that the mineral phase containing Mg ions and nucleated on the natural template (collagen) is non-stoichiometric and remarkably amorphous resembling very well the features of newly deposited bone mineral.…”

Section: Development and Characterization Of The Lower (Bony) And Intmentioning

confidence: 56%

Design of graded biomimetic osteochondral composite scaffolds

et al. 2008

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With the ultimate goal to generate suitable materials for the repair of osteochondral defects, in this work we aimed at developing composite osteochondral scaffolds organized in different integrated layers, with features which are biomimetic for articular cartilage and subchondral bone and can differentially support formation of such tissues.A biologically inspired mineralization process was first developed to nucleate Mg-doped hydroxyapatite crystals on type I collagen fibers during their self assembling. The resulting mineral phase was non-stoichiometric and amorphous, resembling chemico-physical features of newly deposited, natural bone matrix. A graded material was then generated, consisting of (i) a lower layer of the developed biomineralized collagen, corresponding to the subchondral bone, (ii) an upper layer of hyaluronic acid-charged collagen, mimicking the cartilaginous region, and (iii) an intermediate layer of the same nature as the biomineralized collagen, but with a lower extent of mineral, resembling the tidemark. The layers were stacked and freeze-dried, to obtain an integrated, monolithic composite. Culture of the material for 2 weeks after loading with articular chondrocytes yielded cartilaginous tissue formation selectively in the upper layer. Conversely, ectopic implantation in nude mice of the material after loading with bone marrow stromal cells resulted in bone formation which remained confined within the lower layer.In conclusion, we developed a composite material with cues which are biomimetic of an osteochondral tissue and with the capacity to differentially support cartilage and bone tissue generation. The results warrant test of the material as a substitute for the repair of osteochondral lesions in orthotopic animal models. Response to Reviewer #1:1) results and discussion should be separated We would prefer to maintain Results and Discussion combined, in order to avoid possible redundancies and improve readability. 2) figures should be detailed with arrows and information about specific orientations. Details and arrows have been included in the Figures3) collagen type 1 was used for the creation of the construct -in every layer? Usually collagen type 1 does not play a role in articular cartilage. This should be addressed in the discussion. According to the Reviewer's comment, a rationale for the use of type I collagen has been included in the text, as follows: "Type I collagen was selected for the synthesis of the composite layers, including the cartilaginous one, due to (i) its good physico-chemical stability and processability and (ii) high safety and biocompatibility profile, related to the removal of all Reply Letter to Editor and referee's comments telopeptides, which are potentially responsible for immunological reactions." (Section 2.1, Page 2, 1 st paragraph). Indeed, type II collagen is normally extracted porcine derivative, and is still not available either in bulk for processing or for clinical use. Nowadays there are only few papers on experimental studies desc...

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“…[18], carbonate-phosphate ratio (890 -850 cm -1 / 1200 -900 cm -1 ) [19], crystallinity (1030/1020 cm -1 ) [20,21], collagen maturity (1660/1690 cm -1 ) [22] and acid phosphate (1127/1096 cm -1 ) [23]. Furthermore, the spatial heterogeneity of each parameter was determined from the full width of half maximum (FWHM) of the Gaussian curve fitted to the pixel histogram of the measured area [24].…”

Section: Ftirmentioning

confidence: 99%