Characterising Bone Material Composition and Structure in the Ovariectomized (OVX) Rat Model of Osteoporosis

Mathavan, Neashan; Turunen, Mikael J.; Tägil, Magnus; Isaksson, Hanna

doi:10.1007/s00223-015-9991-7

Cited by 60 publications

(34 citation statements)

References 39 publications

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“…In osteoporosis, bone-formation capacity is weakened or bone-resorption capacity is enhanced relatively3738. CPC has been used for treatment of non-load-bearing bone defects by virtue of its excellent bioactivity, biocompatibility and osteoconductivity, but it does not have special advantages for osteoporosis treatment.…”

Section: Discussionmentioning

confidence: 99%

Acceleration of bone regeneration by activating Wnt/β-catenin signalling pathway via lithium released from lithium chloride/calcium phosphate cement in osteoporosis

Peng

Qin

et al. 2017

Sci Rep

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By virtue of its excellent bioactivity and osteoconductivity, calcium phosphate cement (CPC) has been applied extensively in bone engineering. Doping a trace element into CPC can change physical characteristics and enhance osteogenesis. The trace element lithium has been demonstrated to stimulate the proliferation and differentiation of osteoblasts. We investigated the fracture-healing effect of osteoporotic defects with lithium-doped calcium phosphate cement (Li/CPC) and the underlying mechanism. Li/CPC bodies immersed in simulated body fluid converted gradually to hydroxyapatite. Li/CPC extracts stimulated the proliferation and differentiation of osteoblasts upon release of lithium ions (Li+) at 25.35 ± 0.12 to 50.74 ± 0.13 mg/l through activation of the Wnt/β-catenin pathway in vitro. We also examined the effect of locally administered Li+ on defects in rat tibia between CPC and Li/CPC in vivo. Micro-computed tomography and histological staining showed that Li/CPC had better osteogenesis by increasing bone mass and promoting repair in defects compared with CPC (P < 0.05). Li/CPC also showed better osteoconductivity and osseointegration. These findings suggest that local release of Li+ from Li/CPC may accelerate bone regeneration from injury through activation of the Wnt/β-catenin pathway in osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Acceleration of bone regeneration by activating Wnt/β-catenin signalling pathway via lithium released from lithium chloride/calcium phosphate cement in osteoporosis

Peng

Qin

et al. 2017

Sci Rep

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“…The ovariectomized rat is a well-established experimental model for the study of osteoporosis and to evaluate the effect of different treatments in this condition (Thompson et al 1995, Peng et al 1997, Mathavan et al 2015. In the present study, the evaluation of the BMD of the distal femoral metaphysis using the dual-energy radiometric absorptiometry technique indicated that OVX was able to reduce femoral BMD as a result of the reduction of oestrogen levels.…”

Section: Discussionmentioning

confidence: 53%

“…, Mathavan et al . ). In the present study, the evaluation of the BMD of the distal femoral metaphysis using the dual‐energy radiometric absorptiometry technique indicated that OVX was able to reduce femoral BMD as a result of the reduction of oestrogen levels.…”

Section: Discussionmentioning

confidence: 97%

Alendronate inhibits osteocyte apoptosis and inflammation via IL‐6, inhibiting bone resorption in periapical lesions of ovariectomized rats

et al. 2019

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Aim To evaluate the effect of alendronate (ALN) on the development of periapical lesions induced in ovariectomized rats. Methodology Twenty‐five rats were divided into three groups: sham (control), ovariectomy (OVX) and OVX + ALN. One day after OVX, animals from the OVX + ALN group received the medication via gavage. After 9 weeks, the first molars of all animals were submitted to periapical lesion induction. After 21 days, the animals were euthanized. Femurs were analysed for bone mineral density. The blocks of bone tissue containing the mandibular first molars were submitted to histotechnical processing and staining with haematoxylin and eosin (HE) for periapical lesion analysis under conventional microscopy. At the same time, the morphometric analysis of the periapical lesion area was performed in the fluorescence mode, as well as the histoenzimology for the quantification of osteoclasts and 4′‐6‐diamidino‐2‐phenylindole staining for the quantification of apoptotic osteocytes. In addition, the first maxillary molars were used for analysis of the gene expression of proinflammatory cytokines (IL‐1β, IL‐6 and TNF‐α) and osteoclastogenesis markers (RANKL/OPG). The results were submitted to ANOVA and Kruskal–Wallis tests and Tukey and Dunn post‐tests (significance level of 5%). Results Ovariectomy reduced bone mineral density of the femur, and treatment with ALN was able to prevent bone loss (P < 0.001). Regarding the microscopic analysis of the periapical region, the sham and OVX + ALN groups had moderately increased periodontal ligament and inflammatory infiltrate, while the OVX group had these parameters increased intensely. The periapical lesions of the OVX group were significantly larger in area in comparison to the other groups (P < 0.001). The OVX group had the largest amount of apoptotic osteocytes, and ALN was able to prevent the apoptosis of these cells, in addition to significantly reducing IL‐6 expression (P < 0.05). OVX and ALN had no effect on RANKL/OPG expression and did not influence the number of osteoclasts around the periapical lesion (P > 0.05). Conclusion The hypoestrogenic condition induced by OVX aggravated bone resorption, inducing the death of osteocytes and provoking larger periapical lesions. ALN treatment inhibited osteocyte apoptosis and inflammation via IL‐6, inhibiting bone resorption in periapical lesions of ovariectomized rats.

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“…Em ratas, a privação de estrógeno parece mimetizar os padrões de perda da massa óssea observados em humanos (Frost e Jee, 1992). A rata ovariectomizada é um modelo animal bem estabelecido e comumente utilizado na área da saúde para investigar os efeitos da osteoporose, bem como das terapias antirreabsortivas (Kalu, 1991;Peng et al, 1997;Kim et al, 2015;Mathavan et al, 2015).…”

Section: Introductionunclassified

“…Representação gráfica da correlação entre área e volume das lesões periapicais ou do espaço do ligamento periodontal.DiscussãoDISCUSSÃOA rata ovariectomizada é um modelo experimental pré-clínico aprovado pela Food and Drug Administration (FDA) para o estudo da osteoporose, como resultado do efeito da redução na produção de estrógeno pelos ovários pós-menopausa e para avaliar como potenciais intervenções podem preservar o metabolismo ósseo, nesta condição(Thompson et al, 1995). É um modelo animal bem estabelecido e comumente utilizado na área da saúde para investigar os efeitos da osteoporose, bem como das terapias antirreabsortivas(Kalu, 1991;Peng et al, 1997;Kim et al, 2015;Mathavan et al, 2015).Roedores ovariectomizados desenvolvem hiperfagia e diminuição do gasto de energia, o que resulta em obesidade e resistência à insulina(Rogers et al, 2009;Zhu et al, 2013). Sabe-se que a ovariectomia promove aumento no peso corporal e atrofia uterina(Chen, G. X. et al, 2014) e, portanto, o monitoramento destes dois índices é de grande importância para confirmação do sucesso do procedimento cirúrgico.…”

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Avaliação dos ossos maxilares e de lesões periapicais induzidas em ratas ovariectomizadas submetidas ou não ao tratamento com bisfosfonato ou com inibidor da catepsina K

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Characterising Bone Material Composition and Structure in the Ovariectomized (OVX) Rat Model of Osteoporosis

Cited by 60 publications

References 39 publications

Acceleration of bone regeneration by activating Wnt/β-catenin signalling pathway via lithium released from lithium chloride/calcium phosphate cement in osteoporosis

Acceleration of bone regeneration by activating Wnt/β-catenin signalling pathway via lithium released from lithium chloride/calcium phosphate cement in osteoporosis

Alendronate inhibits osteocyte apoptosis and inflammation via IL‐6, inhibiting bone resorption in periapical lesions of ovariectomized rats

Avaliação dos ossos maxilares e de lesões periapicais induzidas em ratas ovariectomizadas submetidas ou não ao tratamento com bisfosfonato ou com inibidor da catepsina K

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