Maturational changes in dentin mineral properties

Abstract

In this study the changes in properties of the maturing mantle and circumpulpal dentin were quantitatively analyzed. Sections from six fetal bovine undecalcified incisors were used. Regions of mantle and circumpulpal dentin of sequential maturation stages were identified on spectroscopic images acquired by Fourier Transform Infrared Imaging. Spectroscopic parameters corresponding to mineral properties at these stages were analyzed and reported as a function of distance from the cervix of the incisor, the latter representing tissue age. Mineral parameters were correlated with distance from the cervix. Values of these parameters in mantle and circumpulpal dentin were compared. A multi-phasic pattern of changes was found for all the parameters examined, with most of the alterations occurring in the initial maturation period. The patterns of temporal variation in mantle and circumpulpal dentin mineral properties show distinct developmental stages and were not identical for the two dentin compartments. The study showed that mineral maturation in dentin is not a linear process and that mantle dentin is developmentally distinct from circumpulpal dentin, presenting at certain stages different physicochemical events during the maturation of the tissue.

Dual Role of the Trps1 Transcription Factor in Dentin Mineralization

“…Mineralization of circumpulpal dentin relies on molecular mechanisms different from those that drive mantle dentin formation (57), as underscored by analyses of human conditions associated with dentin defects. In hypophosphatemia caused by either PHEX or VDR mutations, formation of circumpulpal dentin is severely disrupted, although mantle dentin is unaffected, demonstrating the crucial role of phosphate homeostasis genes in the later stages of dentin formation (28, 58 -62).…”

Dentine bonding agents comprising calcium-silicates to support proactive dental care: Origins, development and future

“…With respect to dentinal structure, the peculiarity is represented by numerous substitutions (i) by hydrogenophosphate (HPO 4 2− ) of XO4 groups and (ii) by carbonate (CO3 2− ) of Y2 and XO4 groups. Lastly, biological minerals tend to attain high crystallinity and a more organized structure on the time scale of days or months rather than years 22) . To this end, Lowenstam and Weiner 23) used transmission electron microscopy to evaluate the ultrastructure of HCAp crystallites and confirmed that the average length and width of crystallites was 20 nm, with an approximate thickness of 2-3 nm, resembling plate-like structures.…”

Characterization of Bone and Bone-Based Graft Materials Using FTIR Spectroscopy

“…1030 cm -1 regarded as representative of specific chemical environments: typically, the sub-band at 1020 cm -1 is associated to nonstoichiometric apatites (containing HPO 4 2-and/or CO 3 2-) and that at 1030 cm -1 , to stoichiometric apatites. Given that the ratio of the areas of the sub-bands at 1020 and 1030 cm -1 has shown to decrease as mineral maturation proceeds, this ratio is often applied to evaluate the maturity of the bone apatite (Miller et al, 2001;Paschalis et al, 2011;Verdelis et al, 2007). by curve-fitting.…”

“…Moreover, this band may also contain a component at ca. 865 cm -1 , due to the contribution of non-apatitic (labile) carbonate (Verdelis et al, 2007).…”