Novel inexpensive fungi proteases: Production by solid state fermentation and characterization

Novelli, Paula Kern; Barros, Margarida Maria; Fleuri, Luciana Francisco

doi:10.1016/j.foodchem.2015.11.089

Cited by 75 publications

(44 citation statements)

References 28 publications

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“…These advantages have raised the interest of researchers to obtain several biocatalysts of industrial interest, such as lipases [14,[32][33][34][35][36][37][38], proteases [39][40][41], cellulases [42,43], xylanases [44,45], pectinases [16,46], amylases [47][48][49], phytases [50][51][52], inulinases [53,54], tannases [55,56], and others [57] (Table 1). The metabolic expression of fungi differs according to the type of the residues used as substrate, which Catalysts 2017, 7, 9 4 of 34 allows the production of enzymes with different features that can be utilized in the biotechnological industry in different forms [58]. Moreover, some biomass characteristics, such as inorganic matter, carbohydrates, protein and lipid content, bulk density, elemental analysis (C, N, H, S), calorific value, particle size distribution, and porosity, alter the performance of the process where they are employed, and should be evaluated according to the purpose of the study [59].…”

Section: Use Of Solid Waste To Obtain Biocatalystsmentioning

confidence: 99%

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

et al. 2017

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Abstract:The establishment of a bioeconomy era requires not only a change of production pattern, but also a deep modernization of the production processes through the implementation of novel methodologies in current industrial units, where waste materials and byproducts can be utilized as starting materials in the production of commodities such as biofuels and other high added value chemicals. The utilization of renewable raw resources and residues from the agro-industries, and their exploitation through various uses and applications through technologies, particularly solid-state fermentation (SSF), are the main focus of this review. The advocacy for biocatalysis in green chemistry and the environmental benefits of bioproduction are very clear, although this kind of industrial process is still an exception and not the rule. Potential and industrial products, such as biocatalysts, animal feed, fermentation medium, biofuels (biodiesel, lignocelulose ethanol, CH 4 , and H 2 ), pharmaceuticals and chemicals are dealt with in this paper. The focus is the utilization of renewable resources and the important role of enzymatic process to support a sustainable green chemical industry.

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Section: Use Of Solid Waste To Obtain Biocatalystsmentioning

confidence: 99%

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

et al. 2017

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“…The next most popular extracellular enzymes include amylases, pectinases, xylanases, and cellulases, which are used for the extraction and clarification of beverages and in the starch, textile, and detergent industries (Bhat 2000;Beg et al 2001). The most studied fungi that have the capacity to produce these complex extracellular enzymes are Aspergillus, Penicillium, and Trichoderma (Chandra et al 2007;Quintanilla et al 2015;Novelli et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Potential of Native Fungal Strains of Agave Residues

et al. 2017

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Twelve strains of fungi from residues of Agave durangensis were isolated and identified by molecular techniques for evaluation of their hydrolytic enzyme production capability. A proportion (50%) of the fungal strains belonged to the Aspergillus genus and the other strains used belonged to Alternaria, Neurospora crassa, Mucor sp, Rhizopus sp., Botryosphaeria sp., and Scytalidium sp. The isolated strains were evaluated for their potential to produce extracellular enzymes using different substrates (cellulose, xylan, inulin, Agave fructans, starch, and tannic acid). It was observed that most of the tested strains were capable of simultaneously secreting cellulases, xylanases, inulinases, fructanases, and laccases. Botryosphaeria sp. ITD-G6 was selected for its evaluation in the production of inulinase, using different substrates. Showing high inulinase activities (5.22 U / mL for Agave waste, 4.37 U/ mL for inulin and 5.00 U / mL for Agave fructans).

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“…Even though a wide variety of microbial proteases are available, the use of these enzymes on industrial scale is still limited by their high production costs and the fact that their activity is often limited to a restricted range of biochemical characteristics [5].…”

Section: Introductionmentioning

confidence: 99%

“…Another important advantage of SSF is the higher growth rate exhibited by fungi on solid substrate as compared to submerged fermentation; the morphology of filamentous fungi allows them to colonize the substrate surface and matrix in search of nutrients, consequently secreting higher levels of metabolites and enzymes [5].…”

Section: Introductionmentioning

confidence: 99%

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Novel Protease from <i>Aspergillus tamarii</i> URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

Silva¹,

Oliveira²,

Souza-Motta³

et al. 2016

AER

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This study reports the protease production from Aspergillus tamarii using agroindustrial residues as substrate for solid-state fermentation (SSF) and biochemical characterization. The highest protease production was obtained using wheat bran as substrate at 72 h fermentation with maximum proteolytic activity of 401.42 U/mL, collagenase of 243.0 U/mL and keratinase of 19.1 U/mL. The protease exhibited K M = 18.7 mg/mL and Vmax = 28.5 mg/mL/min. The optimal pH was 8.0 and stable in a wide pH range (5.0 -11.0) during 24 h. The optimum temperature was 40˚C. The proteolytic activity was inhibited by Cu 2+ (33.98%) and Hg 2+ (22.69%). The enzyme was also inhibited by PMSF (65.11%), indicating that is a Serine Protease. These properties suggest that alkaline protease from A. tamarii URM4634 is suitable for application in food industries and leather processing. Additionally, the present findings opened new vistas in the utilization of wheat bran and other effective agroindustrial wastes as substrates for SSF.

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Novel inexpensive fungi proteases: Production by solid state fermentation and characterization

Cited by 75 publications

References 28 publications

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Enzymatic Potential of Native Fungal Strains of Agave Residues

Novel Protease from <i>Aspergillus tamarii</i> URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

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Novel inexpensive fungi proteases: Production by solid state fermentation and characterization

Cited by 75 publications

References 28 publications

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Enzymatic Potential of Native Fungal Strains of Agave Residues

Novel Protease from &lt;i&gt;Aspergillus tamarii&lt;/i&gt; URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

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Novel Protease from <i>Aspergillus tamarii</i> URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation