Novel Protease from <i>Aspergillus tamarii</i> URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation

Abstract

This study reports the protease production from Aspergillus tamarii using agroindustrial residues as substrate for solid-state fermentation (SSF) and biochemical characterization. The highest protease production was obtained using wheat bran as substrate at 72 h fermentation with maximum proteolytic activity of 401.42 U/mL, collagenase of 243.0 U/mL and keratinase of 19.1 U/mL. The protease exhibited K M = 18.7 mg/mL and Vmax = 28.5 mg/mL/min. The optimal pH was 8.0 and stable in a wide pH range (5.0 -11.0) during 24 h. The optimum temperature was 40˚C. The proteolytic activity was inhibited by Cu 2+ (33.98%) and Hg 2+ (22.69%). The enzyme was also inhibited by PMSF (65.11%), indicating that is a Serine Protease. These properties suggest that alkaline protease from A. tamarii URM4634 is suitable for application in food industries and leather processing. Additionally, the present findings opened new vistas in the utilization of wheat bran and other effective agroindustrial wastes as substrates for SSF.

Biochemical characterization and kinetic/thermodynamic study of Aspergillus tamarii URM4634 β‐fructofuranosidase with transfructosylating activity

“…This genus is deeply explored to produce industrial enzymes due to the ability of its members to grow on solid or liquid substrates and to largely produce extracellular enzymes. Moreover, some Aspergillus strains are generally recognized as safe (GRAS) microorganisms, according to the Food and Drug Administration, and used for human and animal nutrition or production of various enzymes used in the food industry …”

“…Moreover, some Aspergillus strains are generally recognized as safe (GRAS) microorganisms, according to the Food and Drug Administration, and used for human and animal nutrition or production of various enzymes used in the food industry. 9 Commercial preparations of enzymes from strains of Aspergillus aculeatus (Pectinex Ultra SP-L 10 and Viscozyme L 11 ) and Aspergillus niger (Rohapect CM 12 ), with high transfructosylating activity have been successfully used to synthesize FOS. Moreover, studies involving the production of FFases were already reported using Aspergillus japonicus, 13 A. niger 14 and other filamentous fungi, such as Penicillium oxalicum, 15 Penicillium citreonigrum, 16 Penicillium sizovae, and Cladosporium cladosporioides, 17 the last two standing out in the production of neo-FOS 18 and the last one in that of FOS using seawater.…”

Purification, Characterization and De-Staining Potentials of a Thermotolerant Protease Produced by Fusarium oxysporum

“…Variations in temperature stabilities could be strain specific and specific enzyme properties molecular characteristics could reveal further these variation. Proteases from other fungi like Aspergillus carneus, Penicillium janthinellum and Aspergillus tamarii showed optimum stability at 40 °C [14,[36][37][38] but unstable at temperatures above 40 °C. Overall, the temperature optima and stability profile of the purified protease suggested that the enzyme could be a viable option for industrial application.…”

Biophysical, photochemical and biochemical characterization of a protease from Aspergillus tamarii URM4634

“…Aspergillus tamarii URM4634 is one strain of fungus isolated from Caatinga, a biome exclusive from Brazil [1], displayed the best performance concerning proteases production among thirty-four GRAS fungal strains [2]. Such a protease displayed collagenolytic and keratinolytic activities after purification, revealing the large applicability of this protease for industrial applications [3].…”