The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Ferreira-Leitão, Viridiana Santana; Cammarota, Magali Christe; Aguieiras, Érika C.G.; Sá, Lívian Ribeiro Vasconcelos de; Fernández-Lafuente, Roberto; Freire, Denise Maria Guimarães

doi:10.3390/catal7010009

Cited by 67 publications

(27 citation statements)

References 207 publications

(217 reference statements)

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“…Fungi degrade first lignin through the action of lignolytic enzymes (lignin peroxidase, manganese peroxidase and laccase) and then can access energy-rich polysaccharides for their metabolism and growth [5]. Enzymatic hydrolysis reactions on the phenolic conjugates generate free phenolics (for example acids like gallic, ferulic and ellagic acids) but also low molecular weight lignin molecules which have a higher antioxidant power [35]. In fruits and vegetables, phenolic compounds can be released from pectins and cellulose using enzymes like -glucosidase [33].…”

Section: Phenols Release From Vegetable Cell Walls Using Fungimentioning

confidence: 99%

Beyond Enzyme Production: Solid State Fermentation (SSF) as an Alternative to Produce Antioxidant Polysaccharides

Gutiérrez-Uribe¹,

Verduzco-Oliva²

2019

Preprint

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Solid state fermentation (SSF) is considered more sustainable than traditional fermentation because it uses low amounts of water and transforms agro-industrial residues into value added products. Enzymes, biofuels, nanoparticles and bioactive compounds can be obtained from SSF. The key factor in SSF processes is the choice of microorganisms and their substrates. Many fungal species can be used and are mainly used due their lower requirements of water, O2 and light. Residues rich in soluble and insoluble fiber are utilized by lignocellulolytic fungi because they have the enzymes that break fiber hard structure (lignases, celullases or hemicelullases). During the hydrolysis of lignin, some phenolic compounds are released but fungi also synthetize compounds such as mycophenolic acid, dicerandrol C, phenylacetates, anthraquinones, benzofurans and alkenyl phenols that have health beneficial effects such as antitumoral, antimicrobial, antioxidant and antiviral activities. Another important group of compounds synthetized by fungi during fermentation are polysaccharides that also have important health promoting properties. Fungal biofermentation has also proved to be a process which can release high contents of phenolics and it also increases the bioactivity of these compounds.

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Section: Phenols Release From Vegetable Cell Walls Using Fungimentioning

confidence: 99%

Beyond Enzyme Production: Solid State Fermentation (SSF) as an Alternative to Produce Antioxidant Polysaccharides

Gutiérrez-Uribe¹,

Verduzco-Oliva²

2019

Preprint

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“…In comparison with the general chemical approach for chiral secondary alcohols synthesis, the biocatalytic method is more preferable because of its high‐substrate specificity, mild reaction conditions, and environmental sustainability. Biocatalysts took advantage of high chemo‐, regio‐, unsurpassed selectivity and observed the green principles . Furthermore, the use of microbial whole cells with metabolic activity as catalysts provides several merits over isolated enzymes, including the cofactor recycling in situ and better protecting target enzymes against inactivation, thereby significantly cutting down the process cost .…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of enantiopure (S)‐1‐(benzofuran‐2‐yl)ethanol by whole‐cell biocatalyst

Şahin

2019

Chirality

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Optically active aromatic alcohols are valuable chiral building blocks of many natural products and chiral drugs. Lactobacillus paracasei BD87E6, which was isolated from a cereal-based fermented beverage, was shown as a biocatalyst for the bioreduction of 1-(benzofuran-2-yl) ethanone to (S)-1-(benzofuran-2-yl) ethanol with highly stereoselectivity. The bioreduction conditions were optimized using L. paracasei BD87E6 to obtain high enantiomeric excess (ee) and conversion. After optimization of the bioreduction conditions, it was shown that the bioreduction of 1-(benzofuran-2-yl)ethanone was performed in mild reaction conditions. The asymmetric bioreduction of the 1-(benzofuran-2-yl)ethanone had reached 92% yield with ee of higher than 99.9% at 6.73 g of substrate. Our study gave the first example for enantiopure production of (S)-1-(benzofuran-2-yl)ethanol by a biological green method.This process is also scalable and has potential in application. In this study, a basic and novel whole-cell mediated biocatalytic method was performed for the enantiopure production of (S)-1-(benzofuran-2-yl)ethanol in the aqueous medium, which empowered the synthesis of a precious chiral intermediary process to be converted into a sophisticated molecule for drug production. KEYWORDS (S)-1-(benzofuran-2-yl)ethanol, asymmetric bioreduction, chirality, Lactobacillus paracasei BD87E6, whole-cell biocatalysts

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“…[1] Mild reactive conditions, ability to process high-acid, low grade, low-cost feedstock, production of a high purity biofuel, and high selectivity of the lipases, which prevents the occurrence of secondary reactions, are some factors that have aroused the interest of researchers about the enzymatic route. [1] Mild reactive conditions, ability to process high-acid, low grade, low-cost feedstock, production of a high purity biofuel, and high selectivity of the lipases, which prevents the occurrence of secondary reactions, are some factors that have aroused the interest of researchers about the enzymatic route.…”

Section: Introductionmentioning

confidence: 99%

Feeding strategies of methanol and lipase on eversa® transform‐mediated hydroesterification for FAME production

et al. 2019

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This work reports the influence of different feeding strategies of methanol and catalysts on fatty acid methyl esters (FAME) yield in a beef tallow hydroesterification reaction for biodiesel production catalyzed by liquid lipase. Soluble lipase from the Thermomyces lanuginosus microorganism, named Eversa 1 Transform, was selected for the reactions in this work. The feeding of methanol and lipase into the system was evaluated varying the methanol to fat molar ratio of 4.0:1 and 4.5:1 and the lipase load of 1.0 wt% and 1.45 wt% in relation to the mass of beef tallow utilized in each assay. The highest yield (85.08 %) of FAME was reached at 35 8C, methanol to fat molar ratio of 4.5:1, 1.0 wt% of lipase, 6.0 wt% of water in only 8 h of reaction, with a one-step addition of lipase and methanol fed to the process at a constant flow of 3.0 g Á h À1 . Moreover, the reutilization process of the liquid lipase after four cycles was evaluated, which is a point rarely addressed in similar works available in the open literature. The findings of this paper demonstrate that the adoption of an appropriate strategy to feed inputs to the system is fundamental in order to take advantage of the maximum catalytic capacity of the lipase and consequently to obtain high yields in the process.

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The Protagonism of Biocatalysis in Green Chemistry and Its Environmental Benefits

Cited by 67 publications

References 207 publications

Beyond Enzyme Production: Solid State Fermentation (SSF) as an Alternative to Produce Antioxidant Polysaccharides

Beyond Enzyme Production: Solid State Fermentation (SSF) as an Alternative to Produce Antioxidant Polysaccharides

Green synthesis of enantiopure (S)‐1‐(benzofuran‐2‐yl)ethanol by whole‐cell biocatalyst

Feeding strategies of methanol and lipase on eversa® transform‐mediated hydroesterification for FAME production

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