Novel exopolysaccharide derived from probiotic Lactobacillus pantheris TCP102 strain with immune-enhancing and anticancer activities

Abstract

Probiotics are gaining attention due to their functions of regulating the intestinal barrier and promoting human health. The production of exopolysaccharide (EPS) is one of the important factors for probiotics to exert beneficial properties. This study aimed to screen exopolysaccharides-producing lactic acid bacteria (LAB) and evaluate the probiotic potential. we obtained three exopolysaccharide fractions (EPS1, EPS2, and EPS3) from Lactobacillus pantheris TCP102 and purified by a combination of ion-exchange chromatography and gel permeation chromatography. The structures of the fractions were characterized by FT-IR, UV, HPLC, and scanning electron microscopy (SEM) analysis. The Mw of EPS1, EPS2, and EPS3 were approximately 20.3, 23.0, and 19.3 kDa, and were mainly composed of galactose, glucose, and mannose, with approximate molar ratios of 2.86:1:1.48, 1.26:1:1, 1.58:1.80:1, respectively. Furthermore, SEM analysis demonstrated that the three polysaccharide fractions differ in microstructure and surface morphology. Additionally, preliminary results for immune-enhancing and anticancer activities reveal that these EPSs significantly induced the production of nitric oxide (NO), TNF-α, and IL-6 in Ana-1 cells and peritoneal macrophage cells. Meanwhile, the EPSs also significantly suppressed the proliferation of HCT-116, BCG-803, and particularly A-2780 cells. The results suggest that the three novel EPSs isolated from Lactobacillus pantheris TCP102 can be regarded as potential application value in functional food and natural antitumor drugs.

Advances in serum‐free media for CHO cells: From traditional serum substitutes to microbial‐derived substances

“…Moreover, many studies have indicated that microbial extracellular polysaccharides possess various potential physiological activities due to their rich structural characteristics and monosaccharide composition, such as antioxidant, anticancer, antibacterial, and immunostimulatory effects. [68] Xiu et al [69] purified a 37.37 kDa polysaccharide from CFS of Lactobacillus casei, mainly composed of glucose, glucosamine, and mannose, and proved its activity in enhancing the proliferation and phagocytic activity of RAW264.7 cells. Moreover, this polysaccharide could induce the maturation and cytokine secretion of mouse dendritic cells, and promote T cell proliferation and IgG-type anti-OVA antibody generation.…”

The impact of microbially modified metabolites associated with obesity and bariatric surgery on antitumor immunity

“…Its antitumor mechanism is mainly realized by strengthening immune regulation, including innate and adaptive immunity ( 141 ). The secretion of interleukin, tumor necrosis factor, interferon and other cytokines that can improve the body’s immune response is accelerated by the activation of immune effector cells (such as macrophages, lymphocytes, NK cells and dendritic cells, 142 , 143 ). Exopolysaccharide can enhance ICB therapy by inducing the production of CCR6+ CD8+ T cells ( 117 ).…”

Lactobacillus plantarum Metabolites Elicit Anticancer Effects by Inhibiting Autophagy-Related Responses

“…The anticancer effects and its underlying mechanisms of L. plantarum and other Lactobacillus species have been previously reported [ 22 , 23 , 24 , 25 ]. L. salivarin REN suppressed the upregulation of COX-2 expression and therefore, decreased the rate of oral carcinogenesis [ 26 , 27 ].…”

“…Many studies have reported L. plantarum effectively suppressed the growth of resistant CRC cells [ 25 , 32 ]. Most of the mechanisms that explain the anticancer effect of L. plantarum have been with a focus on apoptosis.…”