Novel biosynthesis of Ag-hydroxyapatite: Structural and spectroscopic characterization

Synthesis of Hydroxyapatite-Ag Composite as Antimicrobial Agent

“… 22 The UV-vis spectrum shown in Figure 1 A shows a marked absorbance at 300 nm, absorbance recorded on the ultraviolet region of the electromagnetic spectrum corresponding to the hydroxyapatite. The infrared spectrum of Figure 1 B shows bands at 560, 600 and 628 cm –1 corresponding to flexural vibrations of the phosphate group (PO 4 −3 ), 13 , 14 , 22 and bands at 961, 1022 and 1090 cm −1 corresponding to symmetric phosphate group vibrations. 13 , 14 The Raman spectrum of the HAp presented bands at 435, 590, 964, 1049 and 1081 cm −1 , the band at 964 cm −1 being the most intense as seen in Figure 1 C , the bands located at 435 and 590 cm −1 can be attributed to the flexural modes of the phosphate group, the 964 cm −1 band is a typical phosphate band associated with carbonated apatite, while the 1049 and 1081 cm −1 bands are associated with the vibration modes of stretching of the phosphate group.…”

“…00-009-0432. 14 HAp exhibits a great affinity with the AgNPs which the results suggest that the adsorption process of the AgNPs was carried out onto the HAp surface, but in this case a peak of silver are present at low intensity. Figure 4 shows the SEM micrographs of 0.5, 1, 2.5 and 5 mM HAp-AgNPs powders, where the effective presence of AgNPs in HAp can be observed, the contrast of AgNPs is clearly shown (in spherical form) adhered to the surface of the HAp.…”

“…10 Therefore, silver containing in HAp composite fusing biocompatibility, great bioactivity, osteoconductive and chemical stability of HAp with the antibacterial effect of Ag to give a great potential application in bone substitute materials or in coating metal implants to prevent implant-related infections. [11][12][13][14][15][16][17][18] There are several methodologies available for the synthesis of silver nanoparticles: reduction in aqueous systems, chemical reactions, photochemical, thermal decomposition of silver compounds, radiation, electrochemical, sonochemical, microwave chemical routes and recently green methodologies. [9][10][11][12][13][14][15][16][17][18][19] Green synthesis methodologies of synthesis of silver nanoparticles have proven to be better practices, allow manipulation, control overgrowth and stabilization, allowing application in nanomedicine, avoiding the use of toxic products in the synthesis protocol.…”

“… 10 Therefore, silver containing in HAp composite fusing biocompatibility, great bioactivity, osteoconductive and chemical stability of HAp with the antibacterial effect of Ag to give a great potential application in bone substitute materials or in coating metal implants to prevent implant-related infections. 11 - 18 …”

“…There are several methodologies available for the synthesis of silver nanoparticles: reduction in aqueous systems, chemical reactions, photochemical, thermal decomposition of silver compounds, radiation, electrochemical, sonochemical, microwave chemical routes and recently green methodologies. 9 - 19 Green synthesis methodologies of synthesis of silver nanoparticles have proven to be better practices, allow manipulation, control overgrowth and stabilization, allowing application in nanomedicine, avoiding the use of toxic products in the synthesis protocol. Gallic acid (GA) has been used as a green reducing agent to synthesize Au and Ag nanoparticles.…”