Novel application of hydrophobin in medical science: a drug carrier for improving serum stability

Abstract

Multiple physiological properties of glucagon-like peptide-1 (GLP-1) ensure that it is a promising drug candidate for the treatment of type 2 diabetes. However, the in vivo half-life of GLP-1 is short because of rapid degradation by dipeptidyl peptidase-IV (DPP-IV) and renal clearance. The poor serum stability of GLP-1 has significantly limited its clinical utility, although many studies are focused on extending the serum stability of this molecule. Hydrophobin, a self-assembling protein, was first applied as drug carrier to stabilize GLP-1 against protease degradation by forming a cavity. The glucose tolerance test clarified that the complex retained blood glucose clearance activity for 72 hours suggesting that this complex might be utilized as a drug candidate administered every 2–3 days. Additionally, it was found that the mutagenesis of hydrophobin preferred a unique pH condition for self-assembly. These findings suggested that hydrophobin might be a powerful tool as a drug carrier or a pH sensitive drug-release compound. The novel pharmaceutical applications of hydrophobin might result in future widespread interest in hydrophobin.

Dual-functional protein for one-step production of a soluble and targeted fluorescent dye

“…For example, only about 11% of RGD-HFBI/BODIPY was degraded after 72 h in vivo. This result was consistent with the previous reports that self-assembled hydrophobin could resist protease degradation both in vitro and in vivo 72 - 75 . After the stability of RGD-HFBI/BODIPY complex was confirmed, we did in vivo imaging using different tumor models.…”

Applications of Hydrophobins in Medical Biotechnolog

“…This implies that hydrophobin will be a strong tool for drug carrier or drug-release compounds. There is a possibility of a reduction in future interest in hydrophobin indicating the novel pharmaceutical applications of hydrophobin (Zhao et al, 2016).…”

Self-assembly molecular mechanism and modification function on material surface of hydrophobin HGFI

“…分布于气生孢子表面的疏水蛋白DewA, 能协助孢子 的萌发和内容物的保护 [22,26~32] ; 分布于稻瘟病菌表面 的疏水蛋白MPG1能够提高菌表面的疏水性, 有助其 在稻叶表面吸附形成附着胞, 增强其对宿主的侵染能 稳定性进而提高其降血糖效果 [19] . .…”

“…. 本课题组 Figure 6 SEM images of the biofilm formed using Staphylococcus aureus ATCC 6538 on the surface of PCL films (small magnifications, A1-D1; large magnification, A2-D2); bare PCL film, (A1, A2); PCL modified by pH, (B1, B2); PCL modified by PA-1, (C1, C2); and PCL modified by HGFI, (D1, D2) [65] 图 7 真菌疏水蛋白作为GLP-1药物包被载体示意图 [19] Figure 7 Scheme illustrating the transport of GLP-1 by hydrophobin and drug stability determination [19] 如图8所示. 这为后期疏水蛋白制剂口服给药方式的研 究提供了理论基础.…”