Not All of the Rare Operational Taxonomic Units (Otus) Play the Same Role in Maintaining Community Stability

Wu, Long‐Fei

doi:10.15666/aeer/1501_105112

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Although high-throughput sequencing of 16S rRNA gene has been widely used to analyze microbiota composition in various environments ( Wu et al, 2017 ; Gong et al, 2021 ), it is difficult to distinguish microorganisms into species or strain level, which hinders a more detailed analysis of the function of gut microbes at species or strain level. Additionally, it is important to further exploit the gut microbiota-derived metabolites of berberine through metabonomics analysis.…”

Section: Discussionmentioning

confidence: 99%

Berberine Relieves Metabolic Syndrome in Mice by Inhibiting Liver Inflammation Caused by a High-Fat Diet and Potential Association With Gut Microbiota

et al. 2022

Front. Microbiol.

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Whether berberine mediates its anti-inflammatory and blood sugar and lipid-lowering effects solely by adjusting the structure of the gut microbiota or by first directly regulating the expression of host pro-inflammatory proteins and activation of macrophages and subsequently acting on gut microbiota, is currently unclear. To clarify the mechanism of berberine-mediated regulation of metabolism, we constructed an obese mouse model using SPF-grade C57BL/6J male mice and conducted a systematic study of liver tissue pathology, inflammatory factor expression, and gut microbiota structure. We screened the gut microbiota targets of berberine and showed that the molecular mechanism of berberine-mediated treatment of metabolic syndrome involves the regulation of gut microbiota structure and the expression of inflammatory factors. Our results revealed that a high-fat diet (HFD) significantly changed mice gut microbiota, thereby probably increasing the level of toxins in the intestine, and triggered the host inflammatory response. The HFD also reduced the proportion of short-chain fatty acid (SCFA)-producing genes, thereby hindering mucosal immunity and cell nutrition, and increased the host inflammatory response and liver fat metabolism disorders. Further, berberine could improve the chronic HFD-induced inflammatory metabolic syndrome to some extent and effectively improved the metabolism of high-fat foods in mice, which correlated with the gut microbiota composition. Taken together, our study may improve our understanding of host-microbe interactions during the treatment of metabolic diseases and provide useful insights into the action mechanism of berberine.

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Section: Discussionmentioning

confidence: 99%

Berberine Relieves Metabolic Syndrome in Mice by Inhibiting Liver Inflammation Caused by a High-Fat Diet and Potential Association With Gut Microbiota

et al. 2022

Front. Microbiol.

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“…Mechanisms forming and maintaining bacterial bio-diversity are the basic issue of microbial ecology (Ni et al, 2014(Ni et al, , 2016Wu et al, 2017). Geographical isolation is one of the primary factors that restrict microbial distribution and emerge a significant distance-decay relationship regarding microbial community similarity (Bell, 2010).…”

Section: Different Of Norfloxacin Resistant Bacteria Among Different Pondsmentioning

confidence: 99%

Norfloxacin Resistant Bacterial Compositions in Sediments of Chinese Subtropical Fish Ponds

Mao¹,

Huang²,

Chen³

et al. 2019

Appl. Ecol. Env. Res.

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Antibiotic resistant bacteria are widely spread in environments. However, the compositions of bacteria with antibiotic resistance in subtropical fish pond sediment are still unclear. In present study, to analyze the compositions of norfloxacin resistant bacteria in subtropical fish pond sediments, we selectively cultured the sediment bacteria collected from six subtropical fish ponds located in southern China using the basic medium with 50 µg/ml of norfloxacin, and analyzed their compositions using MiSeq high-throughput sequencing of 16S rRNA gene amplicons. Our results showed that various norfloxacin resistant bacteria existed in the fish pond sediments. And most of them were Gram-negative bacteria. Their spatial distribution was mainly influenced by environmental heterogeneity of the ponds. These results implied that norfloxacin resistance was widespread in Gram-negative bacteria in the fish pond sediments located in southern China. These finding provided reference information to prevent fish infected diseases and to assess the risk of antibiotic resistant bacteria in the fish pond sediments.

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“…Genomic DNA of the water microbiota was extracted using the standard phenolchloroform method (Wu et al, 2017). The extracted DNA was diluted to 10 ng/µL and stored at −40 °C for downstream use.…”

Section: Dna Extraction and High-throughput Sequencing Of Prokaryotic 16s Rrna Genementioning

confidence: 99%

Compositions of Prokaryote Communites and Their Relationship to Physiochemical Factors in December in Chaohu Lake and Three Urban Rivers in China

Wu¹

2019

Appl. Ecol. Env. Res.

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The aim of this study was to determine the impacts of anthropogenic disturbances on compositions of prokaryote communites (CPCs) in Chaohu Lake and its three urban tributaries, in China. Prokaryotic diversity in Chaohu Lake and its tributaries was high, and prokaryotic communities showed lower richness in Nanfei River than in Zhegao River and Hangbu River, with waterbody nutrient levels negatively corresponding to prokaryotic diversity. Except for a few rare prokaryotes, or where classification was unclear, the prokaryotes were distributed into 13 phyla and 924 genera. The dominant prokaryotic phyla were Proteobacteria (31.51%), Bacteroidetes (26.50%), Cyanobacteria (19.05%), and Actinobacteria (10.71%). Redundancy analysis (RDA) results showed CPCs, as well as abundances of Proteobacteria, Actinobacteria, and Firmicutes to be primarily affected by the concentration of total phosphorus (TP). Furthermore, Cyanobacteria showed a significant correlation with the concentrations of total nitrogen (TN), nitrate (NO3) and ammonia nitrogen (NH4). pH was correlated with Planctomycetes, while Thermotogae was generally associated with water temperature (WT). These results suggest that trophic status could play an important role in shaping CPCs in both freshwater lakes and rivers. Ultimately, urbanization was found to cause the deterioration of water quality within Chaohu Lake and its tributaries.

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Not All of the Rare Operational Taxonomic Units (Otus) Play the Same Role in Maintaining Community Stability

Cited by 3 publications

References 24 publications

Berberine Relieves Metabolic Syndrome in Mice by Inhibiting Liver Inflammation Caused by a High-Fat Diet and Potential Association With Gut Microbiota

Berberine Relieves Metabolic Syndrome in Mice by Inhibiting Liver Inflammation Caused by a High-Fat Diet and Potential Association With Gut Microbiota

Norfloxacin Resistant Bacterial Compositions in Sediments of Chinese Subtropical Fish Ponds

Compositions of Prokaryote Communites and Their Relationship to Physiochemical Factors in December in Chaohu Lake and Three Urban Rivers in China

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