Norfloxacin Resistant Bacterial Compositions in Sediments of Chinese Subtropical Fish Ponds

Mao, Lutian; Huang, Jiaofang; Chen, Z G; L, X; Liu, H R

doi:10.15666/aeer/1701_10391048

Cited by 7 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LEfSe method is widely used for the comparative analysis of microbiota compositions in various habitats and for the screening of significantly different microorganisms (Xiang et al, 2018;Li et al, 2019b;Li et al, 2019c;Mao et al, 2019;Liu et al, 2022). However, our results showed that this method could not simultaneously compare the differences between two or more treatment groups and the control.…”

Section: Discussionmentioning

confidence: 66%

Strengthening and microbial regulation mechanism of Bacillus on purification device for grass carp culture wastewater

Gao

et al. 2023

Front. Environ. Sci.

View full text Add to dashboard Cite

Aquaculture wastewater (AW) poses a threat to natural aquatic environments. Microecological agents are widely used to regulate and purify AW, with Bacillus being the most common. To evaluate the AW purification effect of adding Bacillus subtilis and Bacillus licheniformis to an AW treatment device, we constructed an experimental device including a small grass carp culture pond and three groups of cuboid reactors. The effects of adding the two strains to the AW treatment reactor on the AW purification effect and the microbiota compositions in the AW and packing surface biofilm were analyzed via high-throughput sequencing of the 16S rRNA gene. Our results showed that adding Bacillus bacteria to reactors improved the total nitrogen (TN) removal efficiency and reduced the chemical oxygen demand (COD). Adding both the B. subtillis and B. licheniformis preparations significantly increased the abundance of Firmicutes in the water microbiota of the reactor at the middle and end stages of the experiment. The addition of Bacillus changed the microbiota composition in the water and packing surface biofilm and significantly increased the abundance of Bacillus at the middle and later stages of the experiment. Therefore, the addition of Bacillus improved the TN removal efficiency in the AW grass carp treatment reactors and significantly reduced the COD in the AW by increasing the abundance of Bacillus and changing the microbiota composition in the system. We provide an effective way for improving the purification capacity of biofilm reactor.

show abstract

Section: Discussionmentioning

confidence: 66%

Strengthening and microbial regulation mechanism of Bacillus on purification device for grass carp culture wastewater

Gao

et al. 2023

Front. Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…7−10 Norfloxacin (NOR) resistance commonly exists in Gram-negative bacteria in the fish pond sediments located in southern China. 11 Therefore, it is crucial to understand the mechanisms of quinolones in aquaculture pathogens.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The misuse of the antibiotics or dosage to treat common aquatic diseases leads to sublethal antibiotic concentrations and causes the development of mutant multidrug resistance strains. , For example, the antibiotic resistance of quinolones has increased following widespread use and appeared in many fish pathogens. − Norfloxacin (NOR) resistance commonly exists in Gram-negative bacteria in the fish pond sediments located in southern China . Therefore, it is crucial to understand the mechanisms of quinolones in aquaculture pathogens.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Reveal the Inherent Antibiotic Resistance Mechanism against Norfloxacin Resistance in Aeromonas hydrophila

et al. 2023

View full text Add to dashboard Cite

Recently, the prevalence of Aeromonas hydrophila antibiotic-resistant strains has been reported in aquaculture, but its intrinsic antibiotic resistance mechanisms are largely unknown. In the present study, a label-free proteomics technology was used to compare the differential protein abundances in response to norfloxacin (NOR) stress in A. hydrophila. The results showed that there were 186 proteins decreasing and 220 proteins increasing abundances in response to NOR stress. Bioinformatics analysis showed that the differentially expressed proteins were enriched in several biological processes, such as sulfur metabolism and homologous recombination. Furthermore, the antibiotic sensitivity assays showed that the deletion of AHA_0904, cirA, and cysI significantly decreased the resistance against NOR, whereas ΔAHA_1239, ΔcysA, ΔcysD, and ΔcysN significantly increased the resistance against NOR. Our results provide insights into NOR resistance mechanisms and indicate that AHA_0904, cirA, AHA_1239, and sulfur metabolism may play important roles in NOR resistance in A. hydrophila.

show abstract

“…To prevent the disease of cultured fish, antibiotics are widely used in pond high-density and intensive culture, which leads to a large number of antibiotics remain in the pond water and sediment (Mao et al 2019). For instance, Wang et al (2011) reported that fish only absorb about 1/5 of antibiotics in feed, and most of the antibiotics enter the water and sediment.…”

Section: Introductionmentioning

confidence: 99%

“…Extensive use of antibiotics has caused a serious threat of antibiotic resistance (Laxminarayan et al 2013). Although the usage of antibiotics in agricultural production is strictly restricted presently, residues of antibiotics in environment and illegal use of antibiotics still cause prevalence of antibiotic resistance, especially in lakes and fish ponds (Hao et al 2017, Liu and Lu 2018, Mao et al 2019, Lemańska et al, 2021. Liu and Lu (2018) reported that Baiyangdian Lake, Taihu Lake, Chaohu Lake, Wulungu Lake, Besiteng Lake, Daliao River were polluted by a variety of antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Archives of Environmental Protection

Mao,

Chen,

Wang

et al. 2022

View full text Add to dashboard Cite

To analyze the composition of norfloxacin-resistant bacteria and norfloxacin-degrading bacteria in pond water and sediment in subtropical China, the composition of antibiotic resistant bacteria in pond water and sediment enriched with norfloxacin-containing medium was analyzed by high-throughput sequencing. Sediment and water samples were collected from 3 fish ponds in subtropical China, and domesticated with norfloxacin, subsequently norfloxacin-resistant bacteria through high-throughput sequencing of 16S rDNA, and isolated norfloxacin--degrading bacteria. Our results showed that the pond sediment and water contain a variety of norfloxacin-resistant bacteria, mainly from Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Chloroflexi. Moreover, we isolated two norfloxacin-degrading bacteria (NorXu-2 and NorXu-3). The norfloxacin-degrading rate by NorXu-2 and NorXu-3 in the culture mediums with 200 μg/mL was the highest, which was up to 49.71% and 35.79%, respectively. When the norfloxacin concentration was 200 μg/mL, NorXu-2 and NorXu-3 had the best norfloxacin--degrading effect at pH of 6, and the degradation rates were 53.64% and 45.54%, respectively. Moreover, NorXu-3 exhibited a good tolerance to high NaCl concentration. These results not only provided basic data for the follow-up study of the molecular mechanism of antimicrobial microbial degradation, but also provided potential norfloxacin degrading bacteria for norfloxacin removal and bioremediation in aquaculture environment.

show abstract

Norfloxacin Resistant Bacterial Compositions in Sediments of Chinese Subtropical Fish Ponds

Cited by 7 publications

References 37 publications

Strengthening and microbial regulation mechanism of Bacillus on purification device for grass carp culture wastewater

Strengthening and microbial regulation mechanism of Bacillus on purification device for grass carp culture wastewater

Quantitative Proteomics Reveal the Inherent Antibiotic Resistance Mechanism against Norfloxacin Resistance in Aeromonas hydrophila

Archives of Environmental Protection

Contact Info

Product

Resources

About