Normative data for arterial blood gas and electrolytes in anesthetized rats

Subramanian, Rajam Krishna; Sidharthan, Anita; Maneksh, Delinda; Ramalingam, Latha; Manickam, A.; Kanthakumar, Praghalathan; Subramani, Sathya

doi:10.4103/0253-7613.106451

Cited by 18 publications

(11 citation statements)

References 3 publications

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“…Nilai normal PaO 2 tikus tidak jauh berbeda dengan manusia. Nilai normal PaO 2 tikus berkisar dari 80,72-109,56 mmHg (Subramanian et al, 2013). Pada penelitian ini terdapat peningkatan MDA pada kelompok tikus pneumotorak.…”

Section: Teknik Pemeriksaan Paounclassified

Pengaruh Durasi Pneumotorak Terhadap Tingkat Stress Oksidatif Paru Tikus Wistar

Muttaqien

Bermansyah²,

Saleh³

2019

Qanun Medika

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Large pneumothorax with long duration of symptoms can be dangerous. Large pneumothorax can cause hypoxemia and its duration states the duration of hypoxia. This long duration of hypoxia can affect changes in tissue conditions of the lungs, which if it reaches 72 hours can increase the permeability of the alveolar capillaries in the lungs to facilitate pulmonary edema. Large pneumothorax with long duration of symptoms can be a risk factor for re-expansion pulmonary edema (REPE). Meanwhile, hypoxia can also cause increased production of free radicals in lung tissue (pulmonary oxidative stress). This study investigated whether the duration of pneumothorax also affects the level of pulmonary oxidative stress, with experiments in rats. There were 4 groups consisting of 6 rats in each group: 24 hours of pneumothorax (A), 48 hours of pneumothorax (B), 72 hours of pneumothorax (C) and control (D). Pneumothorax is made by injecting air into the right pleural cavity of the chest, then we performed X-ray. All samples were examined for PaO 2 to ensure hypoxia status. After that, the rats were examined for malondialdehyde (MDA) levels to express the level of oxidative stress. The result showed that all pneumothorax groups were hypoxemic (PaO 2 below 80 mmHg). Mean MDA levels were higher in pneumothorax groups. However, only group C were significantly higher (p=0,031). MDA levels were 1,601 ± 0,739 in group A, 1,585 ± 0,714 in group B, 2,256 ± 0,513 in group C, and 1,243 ± 0,162 in group D. We concluded that pneumothorax can cause pulmonary oxidative stress if the pneumothorax has a large volume and the duration of symptoms reaches 72 hours.

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Section: Teknik Pemeriksaan Paounclassified

Pengaruh Durasi Pneumotorak Terhadap Tingkat Stress Oksidatif Paru Tikus Wistar

Muttaqien

Bermansyah²,

Saleh³

2019

Qanun Medika

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“…Normative data regarding arterial acid-base balance and plasma ion concentrations would help to identify healthy animals suitable for experiments [1], and there are studies that have examined the reliability of these data [61]. Tables 1 and 2 summarize the ranges of some acid-base balance parameters and ion concentrations in arterial rat blood, which have been described in several published studies.…”

Section: Chronobiology Of Anesthesiamentioning

confidence: 99%

“…These models are used to analyze basic physiological mechanisms, for preclinical and toxicological studies and/or the evaluation of therapeutic approaches [1,2]. Rats are also useful model animals for studying acid-base balance, especially in relation to the cardiovascular and respiratory systems [1].…”

Section: Introductionmentioning

confidence: 99%

Chronobiology of Acid-Base Balance under General Anesthesia in Rat Model

Švorc¹

2018

Circadian Rhythm - Cellular and Molecular Mechanisms

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The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using 3-to 4-monthold pentobarbital(P)-, ketamine/xylazine(K/X)-, and zoletil(Z)-anesthetized female Wistar rats after a 4-week adaptation to an LD cycle (12 h light and 12 h dark). We concluded that P anesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anesthesia. P anesthesia is not the most appropriate type of anesthesia in rat chronobiological experiments. It eliminated LD differences and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance.

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“…Rats are useful models to study functions of various systems in preclinical trials and toxicological studies [1]. Normative data for pH, arterial blood gases and electrolytes would help identify healthy animals for the experiments.…”

Section: Introductionmentioning

confidence: 99%

Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study

Švorc¹

2018

AAP

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The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three-to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)-and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.

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Normative data for arterial blood gas and electrolytes in anesthetized rats

Cited by 18 publications

References 3 publications

Pengaruh Durasi Pneumotorak Terhadap Tingkat Stress Oksidatif Paru Tikus Wistar

Pengaruh Durasi Pneumotorak Terhadap Tingkat Stress Oksidatif Paru Tikus Wistar

Chronobiology of Acid-Base Balance under General Anesthesia in Rat Model

Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study

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