Normal Seedlings as A New Parameter for Predicting Cross-Incompatibility Level on Sweetpotato

Abstract

This research aimed to investigate the incompatibility levels of controlled cross-pollination in sweetpotato, based on normal seedling percentage and their correlation with seed vigour. The controlled cross-pollination in sweetpotato faces issues due to its cross-incompatibility and selfincompatibility characteristics. Currently, the incompatibility level in sweetpotato is investigated based on the fruit set percentage. However, this criterion lacks the ability in accurately predicting the number of new clones. Therefore, it is essential to study new parameters to create a better investigation of incompatibility in sweetpotato. The materials used in this research consisted of eight sweetpotato clones as female and four sweetpotato clones as male parents. Cross-pollinations were done reciprocally. The experiment was conducted at Indonesian Legume and Tuber Crops Research Institute from April to December 2014. The result showed that totally 5,188 times crossing produced about 25% fruit sets and 10% normal seedlings. The use of normal seedlings percentage as a new parameter in evaluating cross-pollination has apparently seemed to be more effective than the fruit sets percentage method because numbers of new clones could be known accurately. This research revealed that the normal seedling could be used as a new parameter in determining the incompatibility level in sweetpotato controlled cross-pollination.

Crossing Among Sixteen Sweet Potato Parents for Establishing Base Populations Breeding

“…However, they were dominated by compatible pairs, and only a small portion was partially incompatible to fully incompatible. Some studies have shown similar results as in this study (Afolabi, Carey, & Akoroda, 2014;Baafi et al, 2016;Indriani, Ashari, Basuki, & Jusuf, 2017;Rahajeng & Rahayuningsih, 2013;Sseruwu, Shanahan, Melis, & Ssemakula, 2016).…”

Clone x Tester Crosses on Compatibility Level of Sweetpotato (Ipomoea batatas L.)

“…Determination of the incompatibility level based on the percentage of normal seedlingswas as follows: compatible if the normal seedlings account for more than 20%, partially compatible if the normal seedlings account betwen 10-20%, very incompatible if the normal seedlings account less than 10%, fully incompatible 0%, if no normal seedlings is produced [9].…”

“…Percentage of fruitset, i.e. the number of fruitsets formed at each cross combination based on Indriani [9]. % of fruit set = (Nf/Ʃ Npf) x 100%…”

“…But in reality the capsules that was formed not all capsule contain of seeds, the percentage of empty seeds from crosses was also very high and seeds that contain embryos do not necessarily grow into normal seedlings. Therefore determining the level of incompatibility in sweet potatoes based on the percentage of normal seedlings will be more accurate [9]. Therefore information on the level of compatibility combination of clones, as male, female and both becomes important in determining the crossing parents.…”

Peluang Heterosis Hasil Persilangan Terkendali pada Bobot Umbi dan Kadar Kalium Ubi Jalar

“…Hasil persilangan terkendali dari enam kombinasi persilangan menunjukkan kompatibel berdasarkan persentase kecambah normal seperti dicantumkan pada Tabel 2. Tingkat inkompatibilitas pada ubi jalar hasil persilangan berdasarkan persentase kecambah normal tergolong kompatibel apabila >20%, kompatibel sebagian (10 -20%), sangat inkompatibel <10% dan inkompatibel penuh bila sama sekali tidak dihasilkan kecambah normal (Indriani et al 2017). Lestari (2010) menyatakan inkompatibilitas dan sterilitas berpengaruh terhadap pembentukan kapsul pada ubi jalar.…”