Nontargeted identification of per- and polyfluoroalkyl substances in human follicular fluid and their blood-follicle transfer

Kang, Qiyue; Gao, Fumei; Zhang, Xiaohua; Wang, Lei; Liu, Jiaying; Fu, Min; Zhang, Shiyi; Wan, Yi; Shen, Huan; Hu, Jianying

doi:10.1016/j.envint.2020.105686

Cited by 66 publications

(47 citation statements)

References 53 publications

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“…The median FF:serum ratio ranged from 0.64 (PFOS) to 1.04 (PFHxS). These values are comparable to previously reported data, 58 a comparison of which can be found in Table S11 in the ESI. † Some PFAS are known to cause developmental toxicity in experimental animals 59 and disturb lipid metabolism in vitro 39,60 and in humans.…”

Section: Tier 1: Prioritization Based On Database Comparisonsupporting

confidence: 92%

Suspect and non-target screening of ovarian follicular fluid and serum – identification of anthropogenic chemicals and investigation of their association to fertility

Hallberg

Plassmann

Olovsson

et al. 2021

Environ. Sci.: Processes Impacts

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Section: Tier 1: Prioritization Based On Database Comparisonsupporting

confidence: 92%

Suspect and non-target screening of ovarian follicular fluid and serum – identification of anthropogenic chemicals and investigation of their association to fertility

Hallberg

Plassmann

Olovsson

et al. 2021

Environ. Sci.: Processes Impacts

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“…Evidence has also shown that ongoing exposure to even relatively low concentrations of PFASs in drinking water increases human serum levels [33]. Second, the blood-follicle transfer e ciencies for PFASs decrease with increasing chain length [34]. Although PFBA was the most abundant PFAS in FF, the present study failed to con rm any association between PFBA and ART outcomes.…”

Section: Discussioncontrasting

confidence: 65%

“…Drinking and dietary intake have been considered the main routes by which PFASs enter the human body [32]. Currently, short-chain PFASs, especially PFBA, have become the major PFAS contaminants in Chinese drinking water, wheat and vegetables [31,33,34,35]. Evidence has also shown that ongoing exposure to even relatively low concentrations of PFASs in drinking water increases human serum levels [33].…”

Section: Discussionmentioning

confidence: 99%

Follicular Fluid Perfluoroalkyl and Polyfluoroalkyl Substances Are Associated With Adverse ART Outcomes in Women With Poor Ovarian Reserve

Cai

Zhong

et al. 2021

Preprint

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BackgroundPrevious evidence suggests that perfluoroalkyl and polyfluoroalkyl substances (PFASs) adversely affect ovarian function and female fecundity. However, the evidence remains insufficient to infer a direct relationship between PFAS exposure and adverse assisted reproductive technology (ART) outcomes. To fill this gap, we examined follicular fluid PFAS exposure and ART outcomes in patients with poor ovarian reserve (POR) in a prospective study.MethodsIn total, 147 women with POR were included. Eight PFASs were measured in follicular fluid (n=104) samples using simultaneous analysis by ultra-performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry. The PFAS contamination status of the patients’ follicular fluid and the association between characteristics and ART outcomes were investigated by logistic regression.ResultsAfter adjustment for age and BMI, PFOA, PFNA, PFHxS and ∑PFAS were strongly associated with a decreased probability of pregnancy (PFOA highest vs. lowest tertile: OR=1.95, 95% CI: 1.61, 2.38; PFNA highest vs. lowest tertile: OR= 3.0, 95% CI: 2.46, 3.68; PFHxS highest vs. lowest tertile: OR= 1.95, 95% CI: 1.61, 2.35; ∑PFAS second vs. lowest tertile: OR=3.31, 95% CI: 2.74, 3.89). PFOS and PFUnDA were inversely associated with failed implantation. No relationships were noted between failed implantation and other PFAS analytes. The same result was obtained when using live birth as an outcome measure.ConclusionsIn women with POR, follicular fluid PFAS exposure may decrease the probability of clinical pregnancy and live birth.

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“…It has been reported that PFOA can enter the follicular fluid through the blood‐egg barrier, causing reproductive toxicity (Kang et al, 2020). Newborn rats injected with PFOA or perfluorooctane sulfonate (PFOS) showed reduced number of growing follicles and secondary follicles, thus reducing the number of oocytes and resulting in long‐term inhibition of oocyte growth (Guizhen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Perfluorooctanoic acid inhibits the maturation rate of mouse oocytes cultured in vitro by triggering mitochondrial and DNA damage

Guo

Zhao

et al. 2021

Birth Defects Research

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Background Perfluorooctanoic acid (PFOA) is widely used in the manufacture of household and industrial products. It has certain toxicity and leaves many residues in the environment. Numerous studies have shown that PFOA exhibits endocrine disrupting properties and immunotoxicity and induces developmental defects. However, there is very little information regarding its toxicity on oocytes. Methods We cultured denuded oocytes in maturation medium supplemented with 0, 300, or 500 PFOA during IVM and evaluated the maturation of oocytes from the aspects of ROS(DCFH‐DA), mitochondria(MitoOrange and JC‐1), DNA damage(P‐H2AX), and cytoskeleton(β‐tubulin). Results Compared with the control group, the PFOA treatment group exhibited significantly reduced proportion of oocytes matutation. Furthermore, the DCFH‐DA test showed that PFOA significantly increased reactive oxygen species (ROS) levels. PFOA disrupted mitochondrial distribution and decreased mitochondrial function as assessed using MitoOrange and JC‐1. In addition, PFOA‐treated oocytes exhibited a significantly higher percentage of P‐H2AX, defective β‐tubulin, abnormal chromosome alignment, lower expression of the anti‐apoptotic gene Bcl‐2, and higher expression of the apoptotic genes caspase3 and Bax. Conclusion In summary, PFOA could negatively and directly affect oocyte maturation in vitro and cause oxidative stress, mitochondrial function disruption, DNA damage, cytoskeleton damage, and apoptosis.

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Nontargeted identification of per- and polyfluoroalkyl substances in human follicular fluid and their blood-follicle transfer

Cited by 66 publications

References 53 publications

Suspect and non-target screening of ovarian follicular fluid and serum – identification of anthropogenic chemicals and investigation of their association to fertility

Suspect and non-target screening of ovarian follicular fluid and serum – identification of anthropogenic chemicals and investigation of their association to fertility

Follicular Fluid Perfluoroalkyl and Polyfluoroalkyl Substances Are Associated With Adverse ART Outcomes in Women With Poor Ovarian Reserve

Perfluorooctanoic acid inhibits the maturation rate of mouse oocytes cultured in vitro by triggering mitochondrial and DNA damage

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