NonSurgical Embryo Recovery from Estrus-Synchronized or Superovulated Morada Nova Ewes: A Feasible Strategy for Sheep Embryo Banking

Arrais, Aline Matos; Mello, Marco Roberto Bourg de; Vergani, Gabriel Brun; Figueira, Lucas Machado; Esteves, S. N.; Pereira, Verônica Schinaider do Amaral; Bartlewski, Pawel M.; Oliveira, M. E. F.; Souza-Fabjan, J. M. G.; Fonseca, J. F. da

doi:10.1089/bio.2020.0125

Cited by 8 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the ewes of the present study, only 60% (G‐9 SOV ) and 67% (G‐12 SOV ) of all recovered structures were viable embryos. These proportions are similar to those reported in previous studies using the same mating strategy in Morada Nova (G‐9 SOV , 60.0% and G‐12 SOV , 70.5%, respectively; Arrais et al., 2021) and Lacaune ewes (G‐9 SOV , 62.7%; Figueira, Alves, Maia, Souza‐Fabjan, Batista, Morais, et al., 2020) superovulated in the same superovulatory pFSH regimen (133 mg of pFSH in decreasing daily doses). In another study, Morada Nova ewes had 46.9% (68/49) of viable embryos after 14 days of P 4 priming and 133 mg of pFSH, followed by laparoscopic insemination performed 50 hr after device removal (Brasil et al., 2016).…”

Section: Discussionsupporting

confidence: 91%

“…The present study confirmed the suitability of NSER for use in superovulated Morada Nova ewes (Arrais et al., 2021). Superovulated Morada Nova ewes that underwent oestrus synchronization with progesterone‐releasing device for 12 days produced more viable embryos and had a higher proportion of blastocysts than the ewes pre‐treated with progesterone for 9 days.…”

Section: Discussionsupporting

confidence: 90%

“…Based on the ultrasonographic detection of CL, 80.5% of viable embryos were recovered from ewes with ≥10 Cl after the superovulatory treatment (48% of flushed ewes). While for conservation purposes, embryo recovery can be conducted even in poor responders or oestrus‐synchronized, non‐superovulated ewes (Arrais et al., 2021), commercial multiple ovulation and embryo transfer (MOET) programmes require eliminating the collections from animals that do not contribute significantly to a final viable embryo yield. As indicated earlier by Oliveira, Ribeiro, et al.…”

Section: Discussionmentioning

confidence: 99%

“…NSER was performed 6–7 days after the onset of oestrus. Cervical assessment, anaesthetic desensitization, clipping and cervical retraction were also performed as previously described (Arrais et al., 2021). After retraction, the Hegar dilators (size 3–4) were used to traverse the cervix.…”

Section: Methodsmentioning

confidence: 99%

“…While the period of progesterone or progestin exposure in Merino (5–14 days, Menchaca et al., 2010) and Santa Inês ewes (6.5 vs 14.5 days, Oliveira et al., 2020) had a limited effect on in vivo embryo production, the 9‐day treatment with medroxyprogesterone acetate was associated with better outcomes compared with the short‐term (6 days) protocol in Lacaune ewes (Figueira, Alves, Maia, Souza‐Fabjan, Batista, Morais, et al, 2020). Results of a preliminary study comparing embryo yields in superovulated and oestrus‐synchronized, non‐superovulated Morada Nova ewes subjected to progesterone‐based oestrus synchronization protocols of varying durations (6, 9 or 12 days) showed that the 9‐ and 12‐day protocols resulted in better embryo recovery rates than the 6‐day regimen (Arrais et al., 2021), but a confirmatory trial is still needed.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non‐surgical uterine flushing

Oliveira

Arrais

Mello

et al. 2022

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9‐day (G‐9SOV, n = 21) or 12‐day (G‐12SOV, n = 21) progesterone (P4)‐based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle‐stimulating hormone (133 mg of pFSH given over 3 days). Non‐surgical embryo recovery (NSER) was performed 6–7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G‐9SOV and G‐12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G‐12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G‐9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G‐12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G‐9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ≥2 mm in diameter) at the sixth pFSH dose (at P4‐device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G‐12SOV. The ewes with ≥10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G‐12SOV group had better superovulatory responses compared with G‐9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ≥10 ovulations are main contributors to viable embryo production in Morada Nova ewes.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%