Reproductive Biotechnologies Applied to the Female Sheep and Goat

Souza-Fabjan, J. M. G.; Alves, Bruno Henrique; Batista, Ríbrio Ivan Tavares Pereira; Pereira, Alexsandra Fernandes; Melo, Luciana Magalhães; Freitas, Vicente José de Figueirêdo; Oliveira, M. E. F.

doi:10.1007/978-3-031-21630-5_1

Cited by 2 publications

(3 citation statements)

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“…The use of slaughterhouse-derived ovaries as a source of COCs allows the production of a high number of embryos of low cost, which are essential for research in the field of reproductive biology, including those studies aiming to improve IVEP outcomes (Paramio and Izquierdo, 2014;Souza-Fabjan et al, 2023). Post-mortem, the oocytes are commonly retrieved by either gently slicing the ovaries, puncturing, or dissecting follicles.…”

Section: Post-mortemmentioning

confidence: 99%

“…Current protocols for IVF in small ruminants are based on concepts established more than 25 years ago, where oocytes in metaphase II are co-incubated with previously selected and capacitated sperm, usually for a period of 12-18 h (Souza-Fabjan et al, 2023). Although this approach maximizes the number of fertilized oocytes, it exposes the presumptive zygotes to a high concentration of ROS generated by active and degenerated sperm, in addition to positively correlating with polyspermy.…”

Section: In Vitro Fertilization (Ivf)mentioning

confidence: 99%

“…However, a significant increase in demand for food is expected in the forthcoming years, and the current efficiency of small ruminant production systems is considered inadequate to meet global demands (Sargison, 2020). Regarding this aspect, reproductive biotechnologies are essential to increase productivity and accelerate the genetic gain of several production species (Paramio and Izquierdo, 2016;Souza-Fabjan et al, 2023). In vitro embryo production (IVEP) is the biotechnology that has grown the most in recent years, contributing to genetic improvement in livestock.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro embryo production in small ruminants: what is still missing?

Souza-Fabjan,

Leal,

Monteiro

et al. 2023

Anim. Reprod.

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In vitro embryo production (IVEP) is an extremely important tool for genetic improvement in livestock and it is the biotechnology that has grown the most recently. However, multiple ovulation followed by embryo transfer is still considered the leading biotechnology for embryo production in small ruminants. This review aimed to identify what is still missing for more efficient diffusion of IVEP in small ruminants, going through the IVEP steps and highlighting the main factors affecting the outcomes. Oocyte quality is essential for the success of IVEP and an aspect to be considered in small ruminants is their reproductive seasonality and strategies to mitigate the effect of season. The logistics for oocyte collection from live females is more complex than in cattle, and tools to simplify this collection system and/or to promote an alternative way of recovering oocytes may be an important point in this scenario. The heterogeneity of oocytes collected from growing follicles in live females or from ovaries collected from abattoirs remains a challenge, and there is a demand to standardize/homogenize the hormonal stimulatory protocols and IVM protocols for each source of oocytes. The use of sexed semen is technically possible, however the low market demand associated with the high costs of the sexing process prevents the routine use of this technique, but its higher availability is an important aspect aiming for greater dissemination of IVEP. New noninvasive approaches for embryo selection are key factors since the selection for transfer or cryopreservation is another difficulty faced among laboratories. Embryo selection is based on morphological traits, although these are not necessarily reliable in predicting pregnancy. Several issues described in this review must be considered by researchers in other to promote the diffusion of IVEP in small ruminants.

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Section: Post-mortemmentioning

confidence: 99%

Section: In Vitro Fertilization (Ivf)mentioning

confidence: 99%