Nonprocessed Adipose Tissue Graft in the Treatment of Dehiscence Bone Defects in Rabbit Tibiae

Matsubara, Fernanda Brugin; Zanicotti, Diogo Godoy; Zielak, João César; Giovanini, Allan Fernando; Gonzaga, Carla Castiglia; Urban, Cı́cero de Andrade; Deliberador, Tatiana Miranda

doi:10.1097/id.0b013e3182563e0d

Cited by 6 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the present results, Matsubara et al (14) demonstrated that non-processed adipose tissue is not an adequate substitute for autogenous bone in the treatment in dehiscent bone defects surrounding implants. This important difference among the results occurred possibly because those authors did not macerate the tissue.…”

Section: Discussioncontrasting

confidence: 99%

“…Recent histological studies (12,13) reported that macerated adipose tissue might favor bone repair when applied to craniofacial defects. On the other hand, Matsubara et al (14) reported the use of adipose tissue graft to treat peri-implant dehiscence, indicating that the presence of adipose tissue could act as a barrier for new bone formation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Osteocalcin Immunohistochemical Expression During Repair of Critical-Sized Bone Defects Treated with Subcutaneous Adipose Tissue in Rat and Rabbit Animal Model

et al. 2013

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate the osteocalcin immunoexpression during the bone repair treated with subcutaneous adipose tissue in rats and rabbits. Fourteen rats and 14 rabbits were used in the study. A critical-sized calvarial defect was created in each animal. The animals were divided into 4 experimental groups: RC (rat control), RbC (rabbit control), RAT (rat adipose tissue), RbAT (rabbit adipose tissue). In the groups RC and RbC the defect was filled with a blood clot. In groups RAT and RbAT, the defect was filled with macerated subcutaneous adipose tissue. The euthanasia was performed at 30 days (RC and RAT) and 40 days (RbC and RbAT). Histological analysis and immunohistochemical analysis of osteocalcin protein expression were performed. Data were submitted to descriptive statistical analysis (mode). Osteocalcin immunoexpression was detected in the experimental models. Notwithstanding, RbAT showed higher positivity, especially in the adipocytes, than the group RAT group. In groups RC and RbC, the surgical wound was filled by collagen fibers. In Group RAT, the defect was composed by collagen fibers and adipocytes in the reparative sites. Similarly, in RbAT, the defect also was partially filled by collagen fibers and presence of adipocytes dispersed among the fibers. Additionally, chronic inflammatory process and areas of bone matrix deposition were observed. It may be concluded that in both animal models the adipose tissue demonstrated low osteogenic capacity. However, the rabbit animal model exhibited a more evident osteocalcin immunoexpression and a greater amount of newly formed bone matrix.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osteocalcin Immunohistochemical Expression During Repair of Critical-Sized Bone Defects Treated with Subcutaneous Adipose Tissue in Rat and Rabbit Animal Model

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Despite all advances in the use of stem cells derived from adipose tissue for the repair of bone defects, there are few studies in the literature that used autogenous adipose tissue as a grafting material for the treatment of maxillary (13,14) and cranial bone defects (15). Matsubara et al (14) also observed that the use of adipose tissue graft did not favor the repair of dehiscence bone defects created surgically in dental implants placed in rabbit tibiae.…”

Section: Introductionmentioning

confidence: 99%

Immunoexpression of PPAR-? and Osteocalcin Proteins for Bone Repair of Critical-Size Defects Treated with Fragmented Autogenous Abdominal Adipose Tissue Graft

et al. 2014

Self Cite

View full text Add to dashboard Cite

Immunoexpression of PPAR-γ and osteocalcin proteins was evaluated for bone repair of critical-size defects (CSDs), created in rat calvaria (n=42) and treated with fragmented abdominal autogenous adipose tissue graft. Three groups (n=14) were formed: C (control - blood clot), AB (autogenous bone) and AT (fragmented adipose tissue). The groups were divided into subgroups (n=7) for euthanasia at 30 and 90 days. Histological and immunohistochemical analyses were performed. Data were subjected to descriptive statistics (mode). A complete bone closure was observed in Group AB 90 days after surgery. In Group C, repair was achieved by the formation of collagen fiber bundles oriented parallel to the wound surface at both post-surgery periods. In Group AT the type of healing was characterized by dense connective tissue containing collagen fiber bundles arranged amidst the remaining adipose tissue, with rare heterotopic bone formation associated with fibrosis and different types of tissue necrosis. Immunostaining of PPAR-γ was not observed in any specimen from Groups C and AB. In Group AT, the immunostaining of PPAR-γ was more evident 30 days after surgery. Immunostaining of osteocalcin was present in all groups and at both postoperative periods. The fragmented autogenous abdominal adipose tissue graft did not favor the repair of critical-size bone defects created surgically in rat calvaria as evidenced by the positive immunostaining of PPAR-γ protein and the negative immunostaining of osteocalcin in the osteoblast-like cells and bone matrix.

show abstract

“…In this context, it is hypothesized that the use of immediate transplant using fragmented subcutaneous white adipose tissue could be a plausible alternative for the treatment of bone deformities (Gomes et al, 2012; Matsubara et al, 2012). Biologically, the premise that the adipose tissue could be used as a regenerative biomaterial is due to the significant presence of stem cells in it (2500-fold more than the frequency of stem cells in bone marrow [Fraser et al, 2008]), which in theory may originate higher quantities of differentiated cells, including osteoblasts (Fraser et al, 2006; Helder et al, 2007).…”

mentioning

confidence: 99%

Fragmented Adipose Tissue Transplanted to Craniofacial Deformities Induces Bone Repair Associated with Immunoexpression of Adiponectin and Parathyroid Hormone 1-Receptor

Azevedo-Neto

Gonzaga

Deliberador

et al. 2013

The Cleft Palate Craniofacial Journal

Self Cite

View full text Add to dashboard Cite

Objective : This study analyzed the influence of autogenous white adipose tissue on bone matrix development in critical-size defects created in rabbit calvaria. Materials and methods : A 15-mm-diameter defect was created in the calvaria of 42 rabbits. Twenty-one rabbits were treated with 86 mm(3) of immediate transplant of fragmented white subcutaneous adipose tissue (WSAT); the others constituted the control group (sham). The animals were euthanized at 7, 15, and 40 days postsurgery (n = 7), and the histological data were analyzed by histomorphometry and immunohistochemistry using the anti-adiponectin and parathyroid hormone 1-receptor (PTH1R) antibodies. Results : The calvariae treated with fragmented WSAT demonstrated significant bone formation. These results coincided with the significant presence of immunopositivity to adiponectin and PTH1R in loci, which in turn coincided with the increase in bonelike matrix deposited both in fat tissue stroma and adipocytes' cytoplasm. In contrast, the control group revealed a small amount of bone-matrix deposition and presented scarce PTH1R expression and a lack of immunostain for adiponectin. Conclusion : These results indicate that transplant of fragmented white subcutaneous adipose tissue may be an alternative to treatment of craniofacial bone deformities because adipose tissue suffers from osseous metaplasia and exhibits immunoexpression of the adiponectin and PTH1R, which are proteins associated with bone metabolism.

show abstract

Nonprocessed Adipose Tissue Graft in the Treatment of Dehiscence Bone Defects in Rabbit Tibiae

Abstract: Nonprocessed adipose tissue is not a comparable substitute for autogenous bone in the treatment of dehiscence bone defects around titanium dental implants.

Cited by 6 publications

References 23 publications

Osteocalcin Immunohistochemical Expression During Repair of Critical-Sized Bone Defects Treated with Subcutaneous Adipose Tissue in Rat and Rabbit Animal Model

Osteocalcin Immunohistochemical Expression During Repair of Critical-Sized Bone Defects Treated with Subcutaneous Adipose Tissue in Rat and Rabbit Animal Model

Immunoexpression of PPAR-? and Osteocalcin Proteins for Bone Repair of Critical-Size Defects Treated with Fragmented Autogenous Abdominal Adipose Tissue Graft

Fragmented Adipose Tissue Transplanted to Craniofacial Deformities Induces Bone Repair Associated with Immunoexpression of Adiponectin and Parathyroid Hormone 1-Receptor

Contact Info

Product

Resources

About