Nonomuraea purpurea sp. nov., an actinomycete isolated from mangrove sediment

Abstract

A polyphasic approach was used to verify the novel actinomycete, strain 1SM4-01T, isolated from mangrove sediment collected from Ranong Province, Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism was a member of the genus Nonomuraea and was most closely related to Nonomuraea syzygii GKU 164T (98.7 % sequence similarity), Nonomuraea rhizophila YIM 67092T (98.4 %), Nonomuraea solani NEAU-Z6T (98.4 %), Nonomuraea monospora PT708T (98.3 %) and Nonomuraea thailandensis KC-061T (98.2 %). The strain produced branching aerial mycelium which differentiated into straight chains of rough-surfaced spores borne at the end of a short sporophore. The whole-cell hydrolysates contained meso-diaminopimelic acid as the diagnostic diamino acid, with madurose, mannose and ribose as the main sugars. MK-9(H4) was a major menaquinone of this strain. The acyl type of peptidoglycan was N-acetyl. The predominant cellular fatty acids were C17 : 1ω8c and iso-C16 : 0. Phospholipids consisted of diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, aminophospholipids and unidentified lipids. The G+C content of the genomic DNA was 70.4 mol%. On the basis of phenotypic characteristics, DNA-DNA relatedness and phylogenetic distinctiveness, strain 1SM4-01T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea purpurea sp. nov. is proposed. The type strain is 1SM4-01T (=BCC 60397T=NBRC 109647T).

Nonomuraea antri sp. nov., an actinomycete isolated from cave soil in Thailand

“…G+C content ranges from 64 to 73 mol%. Members of the genus are widely distributed and have been isolated from various natural habitats such as plant roots, terrestrial soils, mangrove sediments and cave soils [3,[6][7][8][9]. At the time of writing, the genus comprises 59 species with validly published names (https:// lpsn.…”

Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.

“…In addition, it is known that Nonomuraea rosea, Nonomuraea roseoviolacea, Nonomuraea rubra, Nonomuraea pusilla, Nonomuraea spiralis and Nonomuraea gerenzanensis produce deoxycephalomycin B, carminomicins, maduromycin, actinotiocin, pyralomicin and glycopeptide antibiotic A40926 [9][10][11][12][13][14][15]. Members of the genus have been isolated from diverse natural habitats such as rhizosphere [16,17], leaf [18], cave [19], limestone mine [20], root [5,21], coastal sediment [22], mangrove sediment [23] and mushroom compost [24], while many members were isolated from different types of soil including cave soil [25], forest soil [26,27], arid soil [28], acidic soil [29], rhizosphere soil [3,[30][31][32] and mangrove soil [33].…”

Nonomuraea nitratireducens sp. nov., a new actinobacterium isolated from Suaeda australis Moq. rhizosphere

“…Members of the genus have been isolated from various habitats such as coast, cave, desert, forest, lime, rhizosphere and swamp, and also obtained as endophytes in many plants. Among them, Nonomuraea mangrovi [3], Nonomuraea maritima [4], Nonomuraea purpurea [5] and Nonomuraea wenchangensis [6] were obtained from saline soil or rhizosphere of halophytes. They are aerobic, Gram-stain-positive, with extensively branched substrate and aerial mycelia.…”