Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine

Abstract

In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric determination of acetylcholine in ampoule, serum and urine samples. The electrochemical behaviour of acetylcholine at the modified electrode was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 130 mV) and an obvious increase in the peak current compared to the non-modified screen-printed electrode. The results indicated that modified screenprinted electrode enhanced electrocatalytic activity towards the oxidation of acetylcholine. Under optimized conditions, the limit of detection from the experiment of acetylcholine determination was 0.02 µM with acetylcholine concentration in range of 0.1-500.0 µM. The reproducibility of the measurements was tested by recording the responses for 50.0 µM acetylcholine with four different developed sensors prepared on the same manner and the same day. A relative standard deviation value of 4.3% was obtained. Finally, a recovery test is done as a part of accuracy evaluation of the system. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

La2O3/Co3O4 nanocomposite modified screen printed electrode for voltammetric determination of sertraline

“…[13][14][15] However, because of the poor electrochemical behaviour of the most of electro--active analytes, low reducibility, sensitivity and high over potential at electrodes, the performance of the electrodes in electrochemical methods can be improved if they were modified by various nanomaterials and methodologies. [16][17][18][19][20][21][22][23][24][25][26][27] Nanostructured materials are one of the most promising supporting materials for surface modification of electrodes, due to their unique properties, such as good biocompatibility, high surface-to-volume ratio, substantial mechanical power and electrical properties. Lanthanum (La) is one of the most important lanthanide elements.…”

“Nano”: An Emerging Avenue in Electrochemical Detection of Neurotransmitters

“…Ion selective electrodes made up of poly­(3,4-ethylenedioxythiophene)/poly­(styrenesulfonate) (PEDOT/PSS) were also used for electrochemical detection of ACh . Screen printed electrodes modified with magnetic core–shell manganese ferrite nanoparticles were successfully employed for ACh detection in serum samples, which represents the next dimension in electrochemical sensing Figure A depicts the scheme for the synthesis of copper cobaltite nanoparticles (CCO NP) synthesized using copper nitrate and cobalt nitrate as precursors.…”

“…62 Screen printed electrodes modified with magnetic core−shell manganese ferrite nanoparticles were successfully employed for ACh detection in serum samples, which represents the next dimension in electrochemical sensing. 63 Figure 2A depicts the scheme for the synthesis of copper cobaltite nanoparticles (CCO NP) synthesized using copper nitrate and cobalt nitrate as precursors. CCO NP were drop-casted on GCE electrode and successfully employed for the detection of ACh with a wide linear range from 0.2 μM to 3500 μM.…”

Hierarchical engineering of Mn2O3/carbon nanostructured electrodes for sensitive screening of acetylcholine in biological samples

“…Various analytical methods have been applied for qualitative and quantitative investigation of ACh molecules in biological samples. Various techniques, such as photoluminescence, 16 electrochemiluminescence, [11][12][13][14] mass spectrometry, 17 ionsensitive field-effect transistors, 18 colorimetry, 15 and electrochemical methods 7,9,19,20 have been implemented to detect the ACh levels. Although most of these techniques show good sensitivity and selectivity in the assays for ACh detection, they suffer from many limitations including complex processes, highly expensive machines, long time detection, lack of portable fabrication, and complicated self-using device.…”