Nonenzymatic all-solid-state coated wire electrode for acetylcholine determination in vitro

He, Cheng; Wang, Zhan; Wang, You; Hu, Ruifen; Li, Guang

doi:10.1016/j.bios.2016.05.077

Cited by 28 publications

(12 citation statements)

References 35 publications

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“…Nerve illnesses, such as Alzheimer's, Parkinson's and Huntington's diseases, Myasthenia Gravis, motor dysfunction, Schizophrenia and progressive dementia, are linked to abnormal acetylcholine levels. [3][4][5] In the previous studies, it was reported that bio-molecules could be determined by using different experimental methods such as Capillary Electrophoresis, Chemiluminescence, Electrochemical Analysis, Fluorescence Analytical Procedure, High-Performance Liquid Chromatography, High Performance Liquid Chromatography Mass Spectrometry, Liquid Chromatography Tandem Mass Spectrometry, Paper Spray Mass Spectrometry, Spectro-photometry, Surface Plasmon Resonance, Thin Layer Chromatography and ultraviolet-visible spectro-photometry. [6][7][8][9][10][11] A sensor is an investigative instrument that combines an element with a physicochemical transducer to recognize a particular component.…”

Section: Introductionmentioning

confidence: 99%

Detection of Acetylcholine in an Enzyme‐Free System Based on a GCE/V2O5 NRs/BPM Modified Sensor

et al. 2022

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In this approach, vanadium pentoxide nanorods (V 2 O 5 NRs) were prepared by utilizing a facile wet-chemical approach (WCA) in an alkaline phase at low temperatures then applied to detect acetylcholine. Various optical techniques, such as Ultra-Violet spectroscopy (UV-Visible), Fourier Transform Infra-red spectroscopy (FTIR), Powder X-ray Diffraction (XRD), Field Emission Scanning Emission Microscopy (FESEM), X-ray Electron Dispersive Spectroscopy (XEDS) and X-ray Photo-electron Spectroscopy (XPS) were applied to analyse the V 2 O 5 NRs for structural, optical, functional, morphological and elemental analyses. Adjustment of the light weight fabrication of NRs onto Glassy carbon electrode (GCE) exhibited sensitive and selective acetylcholine sensor probe. Based on the stable current-voltage relationship, analytical sensing parameters like sensitivity, Linear dynamic range (LDR), Limit of detection (LOD), Limit of quantification (LOQ), durability and interference measurement of the fabricated sensor (GCE/V 2 O 5 NRs/BPM) for acetylcholine detection were evaluated and reported in this paper. Here, Nafion was used as a binding polymer matrix (BPM) for the fabrication of flat GCE with V 2 O 5 NRs. Acetylcholine calibration curve was found to be linear throughout in a broad range of concentration of target acetylcholine analyte. Calibration curve was employed to calculate the sensing parameters such as sensitivity (101.27 nAμM À 1 cm À 2 ), LOD down to 11.58 pM, LOQ (38.60 pM) and LDR (100 pM ∼ 100 μM) of the developed GCE/V 2 O 5 NRs/BPM sensor probe. The use of the facile WCA method for the synthesis of low-dimensional V 2 O 5 NRs is a good strategy for developing an un-doped nanomaterial based sensor for enzyme-free bio-molecule recognition and detection via this electrochemical approach. The developed (GCE/V 2 O 5 NRs/BPM) sensor probe could be used to selectively detect acetylcholine in real biological samples by electrochemical method with satisfactory outcomes.

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Section: Introductionmentioning

confidence: 99%

Detection of Acetylcholine in an Enzyme‐Free System Based on a GCE/V2O5 NRs/BPM Modified Sensor

et al. 2022

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“…By contrast, potentiometric determination with ion-selective electrodes (ISEs) has many more advantages. For instance, no operating potential and no enzyme are required, they allow easy storage, and they have relatively long life spans of at least one month [ 25 , 26 ]. In contrast to conventional HA electrodes, which are internally filled with electrolyte solution, the solid conductive layer in solid-contact ISEs transduces ionic signals into electronic conductivity.…”

Section: Introductionmentioning

confidence: 99%

Solid-Contact Ion-Selective Electrodes for Histamine Determination

You

Zhang

et al. 2021

Sensors

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Solid-contact ion-selective electrodes for histamine (HA) determination were fabricated and studied. Gold wire (0.5 mm diameter) was coated with poly(3,4-ethlenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS) as a solid conductive layer. The polyvinyl chloride matrix embedded with 5,10,15,20-tetraphenyl(porphyrinato)iron(iii) chloride as an ionophore, 2-nitrophenyloctyl ether as a plasticizer and potassium tetrakis(p-chlorophenyl) borate as an ion exchanger was used to cover the PEDOT:PSS layer as a selective membrane. The characteristics of the HA electrodes were also investigated. The detection limit of 8.58 × 10−6 M, the fast response time of less than 5 s, the good reproducibility, the long-term stability and the selectivity in the presence of common interferences in biological fluids were satisfactory. The electrode also performed stably in the pH range of 7–8 and the temperature range of 35–41 °C. Additionally, the recovery rate of 99.7% in artificial cerebrospinal fluid showed the potential for the electrode to be used in biological applications.

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“…With their advantages including easy storage, low power consumption, and no need for enzymes and redox potential, several potentiometric ion-selective electrodes (ISEs) for analyses in actual samples have been developed in recent years [23,24]. However, the amount of Ch ISEs is still inadequate.…”

Section: Introductionmentioning

confidence: 99%

Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro

Wang

Zou

et al. 2021

Applied Sciences

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Protoporphyrin IX-based all-solid-state choline (Ch) ion-selective electrodes (ISEs) were fabricated and characterized. Poly (3,4-ethylene dioxythiophene) doped with poly (styrene sulfonate) (PEDOT/PSS) functioning as an ion-to-electron transducer was electropolymerized on the gold wire (0.5 mm diameter). The conductive polymer was covered with a Ch selective membrane containing protoporphyrin IX as an ionophore, which exhibited a lower detection limit of 0.49 μM with the potentiometric method. The Ch sensor performed a wide linear range from 1 μM to 1 mM, a fast response time of less than 5 s, and a decent selectivity of common inorganic and organic ions in the human body. Characteristics such as pH and temperature stability, life span, reproducibility and repeatability were also investigated to be satisfied. With the background of artificial cerebrospinal fluid, the recovery rate in 10−5 M of Ch solution was measured by the standard addition method, revealing the potential for biological application.

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Nonenzymatic all-solid-state coated wire electrode for acetylcholine determination in vitro

Cited by 28 publications

References 35 publications

Detection of Acetylcholine in an Enzyme‐Free System Based on a GCE/V2O5 NRs/BPM Modified Sensor

Detection of Acetylcholine in an Enzyme‐Free System Based on a GCE/V2O5 NRs/BPM Modified Sensor

Solid-Contact Ion-Selective Electrodes for Histamine Determination

Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro

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