Nondistractive extraction of intracellular molecules from yeast using PEF‐assisted autolysis

Okamoto, Shuji; Murakami, Yoji; Urabe, Gen; Katsuki, S.

doi:10.1002/eej.23372

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…PEF has been shown to disrupt fungal cells, such as Candida albicans [65]. Additionally, PEF-assisted autolysis has been explored as a method for the nondestructive extraction of intracellular molecules from yeast, indicating the disruptive potential of PEF on fungal cells [66].…”

Section: Pulsed Electric Fieldsmentioning

confidence: 99%

Green Technology for Fungal Protein Extraction—A Review

Ahmed,

Suzauddula,

Akter

et al. 2024

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Fungal proteins are highlighted for their nutritional value and bioactive properties, making them a significant alternative to traditional protein sources. This review evaluates various green extraction technologies, including enzymatic-, ultrasound-, higher-pressure homogenization-, microwave-assisted, pulsed electric fields-, and supercritical fluid-assisted extraction, focusing on their effectiveness in disrupting fungal cell walls and preserving protein integrity. The findings indicate that these technologies could have the potential to improve protein yield and quality, addressing the challenges posed by fungal cell walls’ complex and resilient structure. The review also underscores the bioactivities of fungal proteins, including antifungal, antibacterial, antioxidant, and anticancer properties. The conclusion emphasises the need for further optimisation and scaling of these technologies, as well as exploring a wider range of fungal species to fully understand their potential as sustainable protein sources. Future research directions include refining extraction methods, integrating multiple approaches, and utilising novel green solvents to maximise efficiency and yield.

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Section: Pulsed Electric Fieldsmentioning

confidence: 99%

Green Technology for Fungal Protein Extraction—A Review

Ahmed,

Suzauddula,

Akter

et al. 2024

Separations

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Selective extraction of biomolecules from Saccharomyces cerevisiae assisted by high-pressure homogenization, pulsed electric fields, and heat treatment: Exploring the effect of endogenous enzymes

Marín-Sánchez,

Berzosa,

Álvarez

et al. 2024

LWT

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Yeast Proteins: Proteomics, Extraction, Modification, Functional Characterization, and Structure: A Review

Zhao,

Han,

Zhu

et al. 2024

J. Agric. Food Chem.

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Proteins are essential for human tissues and organs, and they require adequate intake for normal physiological functions. With a growing global population, protein demand rises annually. Traditional animal and plant protein sources rely heavily on land and water, making it difficult to meet the increasing demand. The high protein content of yeast and the complete range of amino acids in yeast proteins make it a high-quality source of supplemental protein. Screening of high-protein yeast strains using proteomics is essential to increase the value of yeast protein resources and to promote the yeast protein industry. However, current yeast extraction methods are mainly alkaline solubilization and acid precipitation; therefore, it is necessary to develop more efficient and environmentally friendly techniques. In addition, the functional properties of yeast proteins limit their application in the food industry. To improve these properties, methods must be selected to modify the secondary and tertiary structures of yeast proteins. This paper explores how proteomic analysis can be used to identify nutrient-rich yeast strains, compares the process of preparing yeast proteins, and investigates how modification methods affect the function and structure of yeast proteins. It provides a theoretical basis for solving the problem of inadequate protein intake in China and explores future prospects.

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Nondistractive extraction of intracellular molecules from yeast using PEF‐assisted autolysis

Cited by 4 publications

References 27 publications

Green Technology for Fungal Protein Extraction—A Review

Green Technology for Fungal Protein Extraction—A Review

Selective extraction of biomolecules from Saccharomyces cerevisiae assisted by high-pressure homogenization, pulsed electric fields, and heat treatment: Exploring the effect of endogenous enzymes

Yeast Proteins: Proteomics, Extraction, Modification, Functional Characterization, and Structure: A Review

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