Nondestructive Estimation of Callus Growth1

Fowler, C. W.; Janick, Jules

doi:10.21273/hortsci.9.6.552

horts

1974

DOI: 10.21273/hortsci.9.6.552

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Nondestructive Estimation of Callus Growth1

C. W. Fowler

Jules Janick

Abstract: A nondestructive technique for determining callus weight is based on estimation of callus diameter.

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1984

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SOMATIC EMBRYOGENESIS OF CARROT IN HORMONE‐FREE MEDIUM: EXTERNAL pH CONTROL OVER MORPHOGENESIS

Smith

Krikorian

1990

American J of Botany

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Cultures of preglobular stage proembryos (PGSPs) were initiated from mechanically wounded mature zygotic embryos of carrot, Daucus carota, on a hormone-free, semisolid medium. These PGSPs have been maintained and multiplied for extended periods without their progression into later embryo stages on the same hormone-free medium containing 1 mM NH4+ as the sole nitrogen source. Sustained maintenance of cultures comprised exclusively of PGSPs was dependent on medium pH throughout the culture period. Best growth and multiplication of PGSP cultures occurred when the pH of unbuffered, hormone-free medium fell from 4.5 to 4 over a 2-week period or when buffered medium was titrated to pH 4. If the hormone-free medium was buffered to sustain a pH at or above 4.5, PGSPs developed into later embryo stages. Maintenance with continuous multiplication of PGSPs occurred equally well on medium containing NH4+ or NH4+ and NO3-, but growth was poor with NO3- alone. Additional observations on the effects of medium components such as various nitrogen sources and levels, sucrose concentration, semisolid supports, type of buffer, borate concentration, activated charcoal, and initial pH that permit optimum maintenance of the PGSPs or foster their continued developmental progression into mature embryos and plantlets are reported. The influence of the pH of the hormone-free medium as a determinant in maintaining cultures as PGSPs or allowing their continued embryonic development are unequivocally demonstrated by gross morphology, scanning electron microscopy, and histological preparations.

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SOMATIC EMBRYOGENESIS OF CARROT IN HORMONE‐FREE MEDIUM: EXTERNAL pH CONTROL OVER MORPHOGENESIS

Smith

Krikorian

1990

American J of Botany

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An improved polyurethane support system for monitoring growth in plant cell cultures

Conner

Meredith

1984

Plant Cell Tiss Organ Cult

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An improved culture system for plant cells that employs filter paper resting on polyurethane saturated with liquid medium is described. It combines a simplified version of the system outlined by Weber and Lark [1979, Theor Appl Genet 55: 81-86] with the method of growth estimation described by , Can J Bot 58 : 2402-2406. The growth of plated cells or callus can be conveniently monitored through repeated non-destructive fresh weight measurements of the filter paper and adhering cells, thereby allowing the construction of a complete growth curve over the course of an experiment. Experiments with 3 Nicotiana genotypes (N. plumbaginifolia Viv., iV. tabacum L. 'SC 58' and N. tabacum 'WI 38') and 3 Vitis vinifera L. genotypes ('Chenin Blanc', 'Dogridge' and 'White Riesling') clearly demonstrate higher growth rates of plated cells on polyurethane supports compared with agar. Further experiments with N. plum. baginifolia illustrate the use of polyurethane supports for culturing cells at low pH (4.0) and the recovery of spent medium for monitoring changes in pH. These features will greatly facilitate quantitative studies of mineral nutrition and metal toxicity in cultured cells. Polyurethane supports also allow the incorporation of conditioned medium or feeder ceils to support the growth of cells at low densities and facilitate the rapid recovery of variant ceils.

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Average tissue diameter as a non-destructive determinant of potato protoplast-derived callus growth

Taylor

Secor

1992

Environmental and Experimental Botany

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Nondestructive Estimation of Callus Growth1

Abstract: A nondestructive technique for determining callus weight is based on estimation of callus diameter.

Cited by 5 publications

References 0 publications

SOMATIC EMBRYOGENESIS OF CARROT IN HORMONE‐FREE MEDIUM: EXTERNAL pH CONTROL OVER MORPHOGENESIS

SOMATIC EMBRYOGENESIS OF CARROT IN HORMONE‐FREE MEDIUM: EXTERNAL pH CONTROL OVER MORPHOGENESIS

An improved polyurethane support system for monitoring growth in plant cell cultures

Average tissue diameter as a non-destructive determinant of potato protoplast-derived callus growth

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