Average tissue diameter as a non-destructive determinant of potato protoplast-derived callus growth

Taylor, Raymond J.; Secor, Gary A.

doi:10.1016/0098-8472(92)90028-z

Cited by 8 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heights of calli were recorded on a scale of 0-2 (0: None, 1: Medium, 2: High). Average calli diameter was determined based on the average of the longest width and length of calli, as suggested by Taylor and Secor (1992). Finally, calli rate of growth index was calculated by multiplying the average diameter and height of calli.…”

Section: Discussionmentioning

confidence: 99%

Effects of Osmolytic Agents on Somatic Embryogenesis of Saffron (<i>Crocus sativus </i>L.)

2015

View full text Add to dashboard Cite

A protocol for callus induction from meristem tissues and subsequent somatic embryo formation were established in this study. Explants were taken from apical and lateral meristems of saffron and these explants were cultured on MS medium supplemented with combinations of 2.4-dichlorophenoxyacetic acid (2.4-D) and Kinetin (Kn). The effects of osmotic agents such as abscisic acid (ABA), polyethylene glycol (PEG) and Gelrite on somatic embryogenesis were also investigated. After 45 and 60 days of culture, calli were induced from apical and lateral meristems, respectively. The apical meristems yielded higher quality calli when compared to the lateral meristems. The highest frequency of callogenesis and the growth rate of callus were achieved from apical meristems on Murashige and Skoogs (MS) medium supplemented with 2.4-D (2 mg/l) and Kinetin (0.5 mg/l). After 45 days of subculture, the segments of nodular calli were transferred to plant growth regulator (PGR)- free media for induction of pre-embryogenesis embryo formation. Pre-matured embryos were cultured on MS medium supplemented with different osmotic agents such as Gelrite, ABA and PEG to study their effects on embryo maturation. Both PEG and ABA proved more effective for somatic embryo maturation as compared to Gelrite.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Osmolytic Agents on Somatic Embryogenesis of Saffron (<i>Crocus sativus </i>L.)

2015

View full text Add to dashboard Cite

show abstract

“…Average calli diameter was determined based on the average of the longest width and length of calli; the rate of calli growth index was calculated by multiplying the average diameter and height of calli as suggested by (Taylor and Secor 1992).…”

Section: Methodsmentioning

confidence: 99%

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

Vahedi

Kalantari

Salami

2014

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA. In some treatments, calli were transferred to organogenesis stage after two subcultures. For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ proved to be the best growth regulator treatment for shoot regeneration from the saffron calli.

show abstract

“…Non-destructive estimations of callus growth have been reported based on the measurement of linear callus dimensions and on the correlation analysis between actual weight and measured quantities, calculated volume or average callus diameter (2, 5 , 8). These techniques also allow to determine the callus growth curve during each subculture using the same experimental units under undisturbed culture conditions (8). Up to now in Pninirs species callus growth has been evaluated measuring fresh or dry weight (1, 3, 4, 6, 7).…”

mentioning

confidence: 99%

Estimation of callus growth from petals of GF677 peach × almond rootstock

Berardi¹,

Ancherani

Rosati

1993

Giornale botanico italiano

View full text Add to dashboard Cite

Average tissue diameter as a non-destructive determinant of potato protoplast-derived callus growth

Cited by 8 publications

References 19 publications

Effects of Osmolytic Agents on Somatic Embryogenesis of Saffron (<i>Crocus sativus </i>L.)

Effects of Osmolytic Agents on Somatic Embryogenesis of Saffron (<i>Crocus sativus </i>L.)

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

Estimation of callus growth from petals of GF677 peach × almond rootstock

Contact Info

Product

Resources

About