Non-specific β-fructofuranosidase (inulase) from Kluyveromyces fragilis: Batch and continous fermentation, simple recovery method and some industrial properties

GrootWassink, J.W.D.; Fleming, Sharon E.

doi:10.1016/0141-0229(80)90007-1

Cited by 66 publications

(25 citation statements)

References 19 publications

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“…bulgaricus ATCC 16045 reported by Kushi et al (2000), which produces an inulinase with a very similar profile to the enzyme produced by the strain CDBB-L-278 (Cruz-Guerrero et al 1995). The two enzymes displayed activity on both inulin and sucrose, which is a common property of yeast inulinases according to reports for the enzyme of other strains of K. marxianus (Snyder & Phaff 1960;GrootWassink & Fleming 1980;Rouwenhorst et al 1988;Kalil et al 2001). However, the capability of these two strains was outstanding and they had low susceptibility to catabolite repression.…”

Section: Discussionmentioning

confidence: 66%

“…The strains K. marxianus NCYC 1429 (ATCC12424) and UCD-C-351 have been reported as good producers of inulinase (Snyder & Phaff 1960;GrootWassink & Fleming 1980;Hewitt & GrootWassink 1984), and a derivate hyperproducer mutant has been obtained from the former (GrootWassink & Hewitt 1983;Tsang & GrootWassink 1988). It was also reported that the strain K. marxianus CDBB-L-278 is a wild hyperproducer of inulinase (Cruz-Guerrero et al 1995).…”

Section: Introductionmentioning

confidence: 94%

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Inulinase-hyperproducing strains of Kluyveromyces sp. isolated from aguamiel (Agave sap) and pulque

Cruz‐Guerrero

Olvera

Garcı́a-Garibay

et al. 2005

World J Microbiol Biotechnol

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Two strains of Kluyveromyces marxianus (A1 and A2) isolated from 'aguamiel' (agave sap) and one strain of K. lactis var. lactis (P7) isolated from 'pulque' (its fermented product), were studied to make a survey of inulinase production. The strains of K. marxianus A1 and A2 were the best producers of inulinase, giving up to 2.5 times more enzyme than the control hyperproducing strain K. marxianus CDBB-L-278, and showed lower catabolic repression than this. One strain isolated from pulque was identified as K. lactis var. lactis and was also an excellent inulinase producer, being the first strain of this species reported as such. These strains were very good inulinase producers and they had low susceptibility to catabolic repression probably because the source from which they were isolated was rich in sucrose and oligofructans. They can be used in the transformation of inulin to produce fructose and/or oligofructans.

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 94%

Inulinase-hyperproducing strains of Kluyveromyces sp. isolated from aguamiel (Agave sap) and pulque

Cruz‐Guerrero

Olvera

Garcı́a-Garibay

et al. 2005

World J Microbiol Biotechnol

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“…Fructose syrups can be produced from inulin either chemically or enzymatically. GROOTWASINK et al [2] reported that total enzymatic hydrolysis within 3 h at 50 "C did not cause any unwanted color formation. Industrially applicable production schemes to produce UHFGS from inulin are mostly done with Helianthus tuberosus [3], Cichorium intybus [4] and Dahlia pinnata [5] tubers.…”

Section: Introductionmentioning

confidence: 98%

Properties and application of inulinase obtained by fermentation of costus (Saussurea lappa) root powder with Aspergillus niger

Viswanathan

Kulkarni

1995

Nahrung

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SummaryAcetone precipitated extracellular inulinase from Aspergillus niger VAN TIECHEM UV 1 1 grown on costus (Kuth; Saussurea lappa) root powder/corn steep liquor medium was found to have a pH optimum of 5.5, temperature optimum of 50 "C and stability at 50 "C for 60 min. Substrate affinity was in the order of inulin > sucrose > raffinose. At 0.001 M concentrations, Mg2+ and CaZ+ were weak activators of inulinase while Fe3+, Cuz+, Zn2+, Co2+ and Mn2+ were found to be inhibitors of inulinase. Hydrolysis of inulin extracted and purified from costus root powder and standard inulin with inulinase under similar conditions yielded 70% fructose and 68% fructose, respectively. Zusammenfassung

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“…For example, it was demonstrated that inulinase synthesis is subject to a mechanism of induction/repression in Kluyveromyces fragilis, Kluyveromyces bulgaricus ATCC 16046, and Aspergillus niger [6,16,17], of induction without catabolic repression in Kluyveromyces marxianus UCD (FST) 55-82 [7], and vice versa in K. marxianus CBS 6556 [18], whereas other strains of this microorganism did not exhibit any induction mechanism [11,19,20]. Finally, Gupta et al [21] observed that glucose was responsible for catabolic repression, whereas sucrose and fructose acted as weaker inducers than inulin in K. fragilis.…”

mentioning

confidence: 99%

Effects of Carbon and Nitrogen Sources and Oxygenation on the Production of Inulinase by Kluyveromyces marxianus

Silva-Santisteban

Converti

Filho

2008

Appl Biochem Biotechnol

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Cultivations of Kluyveromyces marxianus var. bulgaricus ATCC 16045 were performed on both minimal and complex media using different carbon and nitrogen sources either in the presence or absence of aeration. The results collected were worked out and compared so as to provide a useful contribution to the optimization of inulinase production. Kinetics of extracellular inulinase release were similar on glucose, fructose, and sucrose. Inulinase was detected at basal level since the beginning of batch runs on these three carbon sources and overproduced after their depletion. The highest inulinase activity in minimal medium containing 10 g/l sucrose (6.4 IU/ml) was obtained at an initial (NH(4))(2)SO(4) concentration of 5 g/l, whereas it was reduced to about one fourth of this value and detected only at the beginning under nitrogen-limited conditions. The best sucrose concentrations for the enzyme production were 30 and 20 g/l in minimal and complex media, yielding 15.4 and 208 IU/ml, respectively. In general, the enzyme activity was much higher in complex than in minimal medium under all conditions. O(2)-enriched air neither improved inulinase production nor prevented ethanol formation.

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Non-specific β-fructofuranosidase (inulase) from Kluyveromyces fragilis: Batch and continous fermentation, simple recovery method and some industrial properties

Cited by 66 publications

References 19 publications

Inulinase-hyperproducing strains of Kluyveromyces sp. isolated from aguamiel (Agave sap) and pulque

Inulinase-hyperproducing strains of Kluyveromyces sp. isolated from aguamiel (Agave sap) and pulque

Properties and application of inulinase obtained by fermentation of costus (Saussurea lappa) root powder with Aspergillus niger

Effects of Carbon and Nitrogen Sources and Oxygenation on the Production of Inulinase by Kluyveromyces marxianus

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