Inulinase-hyperproducing strains of Kluyveromyces sp. isolated from aguamiel (Agave sap) and pulque

Abstract

Two strains of Kluyveromyces marxianus (A1 and A2) isolated from 'aguamiel' (agave sap) and one strain of K. lactis var. lactis (P7) isolated from 'pulque' (its fermented product), were studied to make a survey of inulinase production. The strains of K. marxianus A1 and A2 were the best producers of inulinase, giving up to 2.5 times more enzyme than the control hyperproducing strain K. marxianus CDBB-L-278, and showed lower catabolic repression than this. One strain isolated from pulque was identified as K. lactis var. lactis and was also an excellent inulinase producer, being the first strain of this species reported as such. These strains were very good inulinase producers and they had low susceptibility to catabolic repression probably because the source from which they were isolated was rich in sucrose and oligofructans. They can be used in the transformation of inulin to produce fructose and/or oligofructans.

Lactase Production by Solid-state Cultivation of Kluyveromyces marxianus CDBBL 278 on an Inert Support: Effect of Inoculum, Buffer, and Nitrogen Source

“…Many studies of K. marxianus have been carried out in liquid culture to investigate biomass or enzyme production [11][12][13][14][15][16][17][18]. However, although the potential applications are numerous, there have been few studies in solid-state culture (SSC), e.g., the production of inulinase [19], the production and optimisation of aroma compounds using factorial design and surface response methodology [20,21], and the production of inulinase and its preliminary characterization using factorial design [22,23].…”

Pre-selection of protective colloids for enhanced viability of Bifidobacterium bifidum following spray-drying and storage, and evaluation of aguamiel as thermoprotective prebiotic

“…Inulin and frutooligosaccharides (FOS) have proven bifidogenic effect (Kaur & Gupta, 2002;Ö zer, Akin, & Ö zer, 2005). ''Aguamiel'' is the sap obtained mainly from the species Agave atrovirens (Estrada-Godina et al, 2001) and contains a considerable amount of FOS (Cruz-Guerrero, Olvera, García-Garibay, & Gó mez-Ruiz, 2006), and can be considered as an interesting prebiotic and potential thermoprotector. The objective of this work was to demonstrate that B. bifidum viability following spray-drying and storage can be enhanced substantially by: (1) pre-selecting protective colloids that require high activation energy during their isothermal drying process (hinder oxygen diffusion through their drying matrices) as microencapsulating agents, and (2) by using aguamiel as thermoprotector prebiotic.…”

Influence of fermentation by different microflora consortia on pulque and pulque bread properties

“…Pulque microflora have been described as containing mainly Lactobacillus spp., S. cerevisiae , Z. mobilis , and L. mesenteroides . Some other specific strains have also been isolated from this medium, such as Kluyveromyces marxianus , L. mesenteroides , and Leuconostoc kimchi . Valadez‐Blanco et al .…”