Cultivations of Kluyveromyces marxianus var. bulgaricus ATCC 16045 were performed on both minimal and complex media using different carbon and nitrogen sources either in the presence or absence of aeration. The results collected were worked out and compared so as to provide a useful contribution to the optimization of inulinase production. Kinetics of extracellular inulinase release were similar on glucose, fructose, and sucrose. Inulinase was detected at basal level since the beginning of batch runs on these three carbon sources and overproduced after their depletion. The highest inulinase activity in minimal medium containing 10 g/l sucrose (6.4 IU/ml) was obtained at an initial (NH(4))(2)SO(4) concentration of 5 g/l, whereas it was reduced to about one fourth of this value and detected only at the beginning under nitrogen-limited conditions. The best sucrose concentrations for the enzyme production were 30 and 20 g/l in minimal and complex media, yielding 15.4 and 208 IU/ml, respectively. In general, the enzyme activity was much higher in complex than in minimal medium under all conditions. O(2)-enriched air neither improved inulinase production nor prevented ethanol formation.
Dedicatorias y AgradecimientosAl Padre, fuente y centro de lo más puro de mi existir, por el amor, la voluntad y la oportunidad de progreso y la tan esperada unicidad.A mis padres: Eloy (in memmoriam) y Yolanda, por la vida, el amor, la confianza y sus oraciones. A mis tíos Gunter y Violeta, Roque y Flor, Oscar y Agusta, y Horacio y Helena, por la ayuda desinteresada y por haber sido por muchos momentos un gran soporte. A mis hermanos: Antonio, Camila y Domingo, por ser compañeros de lucha, por el esfuerzo constante de entenderme y por la seguridad que tengo de su amor. A Geraldo y Francisca Duim por haberme adoptado con tanto cariño.A mi país, Perú, por ser la fuente inagotable de inspiración en el logro de mis metas personales.A la Fundação de Amparo a Pesquisa do Estado de São Paulo por la confianza puesta en mí al otorgarme la beca para concretar estos estudios de doctorado y por su fe puesta en los estudiantes extranjeros en las universidades brasileñas.Al Prof. Dr. Francisco Maugeri por la confianza puesta en mí y la oportunidad de realizar este trabajo, los consejos, y la motivación constante.
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