Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release

Witzler, Markus; Ottensmeyer, Patrick Frank; Gericke, Martin; Heinze, Thomas; Tobiasch, Edda; Schulze, Margit

doi:10.3390/ijms20143565

Cited by 35 publications

(34 citation statements)

References 56 publications

(79 reference statements)

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“…As a carrier, agarose hydrogel can hold drug molecules and control their release due to the desirable hydrophilicity, porosity, and biodegradability . Moreover, agarose hydrogel is non‐toxic, inert, and biocompatible, which meets the principles of biomaterial design . From the above points of view, we employed agarose hydrogels as encapsulated matrixes.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of porous bovine pericardium scaffolds incorporated with bFGF for tissue engineering applications

Liu

et al. 2019

Xenotransplantation

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Background The design and fabrication of porous scaffolds are important issues for tissue engineering applications. In this study, we attempted to fabricate porous scaffolds using bovine pericardium (BP) and examined whether these scaffolds were beneficial for cell ingrowth and bioactive factors delivery. Methods A vacuum‐freeze‐thawing‐Triton X‐100 (VFTT) protocol was used to fabricate porous BP scaffolds. The porous and mechanical properties were assessed using histology, scanning electron microscopy, and mechanical assay. The fabricated scaffolds were seeded with mesenchymal stem cells (MSCs), and cell ingrowth was evaluated. Basic fibroblast growth factor (bFGF) was subsequently incorporated into the fabricated scaffolds. The bioactive factor delivery capacity was evaluated using loading and release studies. The bioactivity of released bFGF was assessed using a rat subcutaneous model. Results The BP scaffolds fabricated by the VFTT protocol displayed interconnected porous structures with porosity of 6.82 ± 1.36%.There were no significant differences in thickness, ultimate load, Young's modulus, and ultimate tensile strength between the fabricated porous BP scaffolds and native BPs (all P > .05). However, the water content of BPs was slightly reduced after VFTT treatment (P < .05). Cell ingrowth analysis showed that the seeded MSCs penetrated into the porous BP scaffolds with time of culture, while MSCs were limited to the surface layers of native BPs. Furthermore, bFGF was observed to be effectively loaded onto and released from the porous BP scaffolds. The released bFGF increased the phosphorylation levels of Akt, ERK 1/2, and MEK1/2, promoted host MSC recruitment, and inhibited myofibroblast differentiation in vivo. Conclusions The porous BP scaffolds fabricated using a VFTT protocol were promising natural scaffolds for tissue engineering applications, since they had considerable mechanical properties as native BPs, supplied porous channels for cell ingrowth, and possessed bioactive factors delivery capability.

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Section: Discussionmentioning

confidence: 99%

Fabrication of porous bovine pericardium scaffolds incorporated with bFGF for tissue engineering applications

Liu

et al. 2019

Xenotransplantation

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“…Finally, other polysaccharides (like dextran, cellulose, starch, carrageenans, or agarose) and proteins (like heparin or chondroitin sulfate) with relevance for BTE, are listed in Table 3 (Chung and Park, 2007;Martins et al, 2009;Garg et al, 2012;Khan and Ahmad, 2013;Sayin et al, 2014;Shi et al, 2016;Farrugia et al, 2018;Nikpour et al, 2018;Sofi et al, 2018;Witzler et al, 2019;Yegappan et al, 2019). Physico-mechanical characteristics of various composites based on natural polymers are compared in Table 4.…”

Section: Sponginmentioning

confidence: 99%

Natural Polymeric Scaffolds in Bone Regeneration

Filippi

Born

Chaaban

et al. 2020

Front. Bioeng. Biotechnol.

223

151

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Despite considerable advances in microsurgical techniques over the past decades, bone tissue remains a challenging arena to obtain a satisfying functional and structural restoration after damage. Through the production of substituting materials mimicking the physical and biological properties of the healthy tissue, tissue engineering strategies address an urgent clinical need for therapeutic alternatives to bone autografts. By virtue of their structural versatility, polymers have a predominant role in generating the biodegradable matrices that hold the cells in situ to sustain the growth of new tissue until integration into the transplantation area (i.e., scaffolds). As compared to synthetic ones, polymers of natural origin generally present superior biocompatibility and bioactivity. Their assembly and further engineering give rise to a wide plethora of advanced supporting materials, accounting for systems based on hydrogels or scaffolds with either fibrous or porous architecture. The present review offers an overview of the various types of natural polymers currently adopted in bone tissue engineering, describing their manufacturing techniques and procedures of functionalization with active biomolecules, and listing the advantages and disadvantages in their respective use in order to critically compare their actual applicability potential. Their combination to other classes of materials (such as micro and nanomaterials) and other innovative strategies to reproduce physiological bone microenvironments in a more faithful way are also illustrated. The regeneration outcomes achieved in vitro and in vivo when the scaffolds are enriched with different cell types, as well as the preliminary clinical applications are presented, before the prospects in this research field are finally discussed. The collection of studies herein considered confirms that advances in natural polymer research will be determinant in designing translatable materials for efficient tissue regeneration with forthcoming impact expected in the treatment of bone defects.

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“…Self-gelling properties and adjustable mechanical stability [220,221] of agarose gels are crucial for their use. For example, non-toxic [222] and biodegradable agarose gels have been effectively used in implantation surgery [219], wound healing, cartilage [223], cardiac, bone and nervous system [224], and regeneration as well as skin tissue engineering [225,226]. These directions are based on tunable features of agarose, which can result in adjustable characteristics similar to native tissues [225].…”

Section: Patentsmentioning

confidence: 99%

Progress in Modern Marine Biomaterials Research

et al. 2020

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The growing demand for new, sophisticated, multifunctional materials has brought natural structural composites into focus, since they underwent a substantial optimization during long evolutionary selection pressure and adaptation processes. Marine biological materials are the most important sources of both inspiration for biomimetics and of raw materials for practical applications in technology and biomedicine. The use of marine natural products as multifunctional biomaterials is currently undergoing a renaissance in the modern materials science. The diversity of marine biomaterials, their forms and fields of application are highlighted in this review. We will discuss the challenges, solutions, and future directions of modern marine biomaterialogy using a thorough analysis of scientific sources over the past ten years.

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Non-Cytotoxic Agarose/Hydroxyapatite Composite Scaffolds for Drug Release

Cited by 35 publications

References 56 publications

Fabrication of porous bovine pericardium scaffolds incorporated with bFGF for tissue engineering applications

Fabrication of porous bovine pericardium scaffolds incorporated with bFGF for tissue engineering applications

Natural Polymeric Scaffolds in Bone Regeneration

Progress in Modern Marine Biomaterials Research

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