Non-chemical alternatives to methyl bromide against Ephestia cautella (Lepidoptera: Pyralidae): microwave and ozone

Abstract

ABSTRACT:Multiple microwave power (MP) has been applied against Ephestia cautella (Walker) at various exposure times. Five seconds exposure time at various MP up to 60% had no effect on insect mortality, by increasing the MP up to 100%, the mortality reached 20% for larvae and 33% for adults. When exposure time was increased to 15 seconds with different MP, mortality reached 100% and 80% at 60% power for adults and larvae, respectively. Eggs of E. cautella were exposed to various MP at different time; the hatchability reached 0.0 % after 10 and 15 seconds at 100% MP. Data showed microwave had no effect on soluble proteins isolated from dates treated by microwave at various time up to 15 seconds, and had no effect on protein pattern on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Ozonation with 2.0 ppm ozone generated with various time 4, 8 and 12 hours, gave 28%, 73%, and 83% mortality against adults, while it gave 10%, 20% and 27% mortality against larvae. Ozone exhibited less effect against pupae and eggs hatchability. The possibility of using microwave and ozone in integrated management program against stored product pests is discussed.

Hot water treatments combined with cold storage as a tool for Ectomyelois ceratoniae mortality and maintenance of Deglet Noor palm date quality

“…These include new techniques that are aimed for the avoidance of postharvest pests and diseases, as well as microbial contamination of dates, and to improve the quality of the product during the storage period. Traditional and new postharvest techniques that have been developed for date fruit include irradiation (Azelmat et al, 2006), microwave (Zouba et al, 2009;Abo-El-Saad et al, 2011), essential oils (Haouel et al, 2010), ozone (Abo-El-Saad et al, 2011;Jemni et al, 2014), vacuum and modified atmosphere packaging (Achour et al, 2003), heat treatments (Ben-Lalli et al, 2013), etc. There has been growing interest in the application of postharvest heat treatments for controlling pests, insects and microbial agents, as a secure and non-chemical method to control postharvest diseases (Lurie, 1998;Spadoni et al, 2013;Strano et al, 2014;Zhou et al, 2014).…”

Comparative Efficacy of CO2and Ozone Gases AgainstEphestia cautella(Lepidoptera: Pyralidae) Larvae Under Different Temperature Regimes

“…2001 ). Ozone (2.0 ppm) efficacy for 12 h exposure showed 83 and 27% mortality of E. cautella adults and larvae, respectively ( Abo-El-Saad et al. 2011 ).…”

Role of Modified Atmosphere in Pest Control and Mechanism of Its Effect on Insects

“…O 3 treatment caused 100% larval mortality of E. cautella after 24-h exposure at two temperature regimes ( Husain et al., 2015 ). O 3 at 2.0 ppm induced 83 and 27% mortality of E. cautella adults and larvae, respectively, after 12-h exposure ( Abo-El-Saad et al., 2011 ). Three-day exposure to 5–45 ppm O 3 led to 92–100% mortality of larvae of Tribolium castaneum Herbst, S. zeamais adults, and P. interpunctella in stored maize ( Kells et al., 2001 ) and other stored products ( Abo-El-Saad et al., 2011 ; Husain et al., 2015 ).…”