Non-aqueous capillary electrophoresis

Riekkola, Marja‐Liisa; Jussila, Matti; Porras, Simo P.; Valkó, István E.

doi:10.1016/s0021-9673(00)00108-4

Cited by 182 publications

(133 citation statements)

References 73 publications

Supporting

Mentioning

132

Contrasting

Unclassified

Order By: Relevance

“…Since acidic-basic property is one of the most important parameters influencing the selectivity of NACE separation [12,29], the effect of apparent pH on the migration time of phospholipids was studied in acetonitrile: 2-propanol (3:2, v/v) and 60 mM ammonium acetate with various fraction of acetic acid from 0% to 5%. The results are shown in Fig.…”

Section: Effect Of Acetic Acidmentioning

confidence: 99%

“…NACE offers excellent selectivity, thus NACE has been successfully applied for the analysis of organic environmental pollutants [12][13][14], drugs and pharmaceuticals [15][16][17][18], plant extracts [19], chirals [20,21], inorganic anions [22,23], organic acids [24] and oligomers [25]. The selectivity of NACE was often realized by manipulating separation medium [26], the additives and their concentrations [27], and electrolyte concentrations [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Guo

Wen

Shan

et al. 2005

Journal of Chromatography A

View full text Add to dashboard Cite

A method has been developed for the separation and determination of phospholipids by nonaqueous capillary electrophoresis in a separation medium of acetonitrile-2-proponol (3:2, v/v), 0.3% acetic acid and 60 mM ammonium acetate. To optimize the separation conditions, the composition of separation medium including alcohols, acetic acid, n-hexane and ammonium acetate was studied. The solvation interaction and ion-dipole interaction were also investigated. The contents of phospholipids in soybean, sunflower, peanut, apricot kernel, filbert and walnut were determined by the recommended method. The results obtained by the nonaqueous capillary electrophoreses were in good agreement with those determined by micellar electrokinetic chromatography.

show abstract

Section: Effect Of Acetic Acidmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Guo

Wen

Shan

et al. 2005

Journal of Chromatography A

View full text Add to dashboard Cite

show abstract

“…ACN has different chemical and physical properties from MeOH, e.g. its dielectric constant-to-viscosity ratio (e/Z 5 110) is larger than that of MeOH (e/Z 5 60) [47]. Thus, in some cases, ACN shows a stronger ability of selectivity manipulation in CE than MeOH.…”

Section: Coating Stability In Mixed Acn-aqueous Solventsmentioning

confidence: 99%

Use of gemini surfactants as semipermanent capillary coatings in aqueous‐organic solvents for capillary electrophoretic separation of inorganic anions

Liu

Yao

et al. 2009

J of Separation Science

View full text Add to dashboard Cite

This paper proposes a new method for CE separation of inorganic anions based on the use of gemini surfactants as capillary coatings in mixed aqueous-organic solvents. The semipermanent gemini surfactant coatings were facilely prepared by rinsing the capillary with 18-s-18 solutions; they can keep be stable during the electrophoretic runs without surfactants in buffer. The coatings showed a good tolerance of methanol (MeOH) or ACN, e.g. at pH 8.0 and with 40% v/v MeOH or ACN, the EOF magnitude after 60 min of continuous electrokinetic rinsing only decreased by 2.9 or 6.0%, respectively. The coatings were successfully applied to the separation of inorganic anions. Adding organic solvents in buffer can effectively improve the resolution and efficiencies; however, it remarkably prolonged the analysis time due to the suppression of EOF. Interestingly, varying the spacer length of the gemini surfactants can also modulate (improve) the resolution but without any sacrifice of analysis time. This benefit was resulted from the unique chemical structures of gemini surfactants because it introduced a new variable, i.e. the spacer length, to the separation mechanism. IntroductionGemini surfactants are a relatively new type of surfactants, which have two hydrophobic chains and two polar headgroups linked by a spacer group [1][2][3]. Compared with the corresponding conventional (monomeric) single-chained surfactants, the gemini surfactants show better water solubility, lower critical micelle concentration, lower Krafft point, and better surface activity in aqueous solutions [2][3][4]. These features make the gemini surfactants very attractive in both academic and industrial worlds. In particular, the spacer group that can be hydrophilic or hydrophobic, flexible or rigid, heteroatomic, aromatic, etc., plays an important role in determining the solution properties of gemini surfactants [5][6][7][8][9]. In principle, the spacer group can connect any two identical or different surfactants among the available ones. Therefore, gemini surfactants are thought to be designable with an enormous variety of structures [3].Although gemini surfactants have recently generated much research interest, their applications in CE are still rare. Gemini surfactants can be good alternatives to other micelle-forming reagents, buffer additives and capillary coatings, thus it is rational to anticipate a bright prospect of application of gemini surfactants in CE. A few earlier reports described the use of gemini surfactants as micelleforming reagents in MEKC, and found that the gemini surfactants exhibit better resolution or wider migration time windows than the commonly used single-chained surfactants [10][11][12]. Warner and coworkers [13][14] and systematically studied the use of anionic gemini surfactants as pseudostationary phases in MEKC, and presented some advantages of anionic gemini surfactants compared with conventionally used SDS. Due to their lower critical micelle concentrations, the gemini surfactants can be used at much lower concentrat...

show abstract

“…However, this approach is not very practical due to Joule heat, and suffers from many restrictions, such as that a non-aqueous buffer has to be used to limit the current in the system. [7][8][9] Alternatively, the openings at both ends of a relatively large capillary can be shrunk to reduce the current. 10 Further, it is possible to use a larger capillary (530 mm) when using a combination packing materials and an organic mobile phase.…”

Section: Introductionmentioning

confidence: 99%

Zone Electrophoresis in an Inner-cooling Wide-bore Electrophoresis System with UV Detection

et al. 2008

View full text Add to dashboard Cite

Experimental Reagents and chemicalsIn this study, all chemicals used were of analytical grade or higher, unless otherwise stated. Benzenesulfonic acid, salicylic acid, p-toluenesulfonic acid, p-aminobenzene sulfonic acid, paminobenzoic acid, phenylamine, ephedrine, 1-naphthylamine, . R. ChinaA novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. <100 mm) to a wide-bore tube (i.d. >1000 mm). The wide tube allows the loading of over 1.0 mL of the sample with an LOD of 3.0 ¥ 10 -4 mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.

show abstract

Non-aqueous capillary electrophoresis

Cited by 182 publications

References 73 publications

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Use of gemini surfactants as semipermanent capillary coatings in aqueous‐organic solvents for capillary electrophoretic separation of inorganic anions

Zone Electrophoresis in an Inner-cooling Wide-bore Electrophoresis System with UV Detection

Contact Info

Product

Resources

About