Separation and determination of phospholipids in plant seeds by nonaqueous capillary electrophoresis

Abstract

A method has been developed for the separation and determination of phospholipids by nonaqueous capillary electrophoresis in a separation medium of acetonitrile-2-proponol (3:2, v/v), 0.3% acetic acid and 60 mM ammonium acetate. To optimize the separation conditions, the composition of separation medium including alcohols, acetic acid, n-hexane and ammonium acetate was studied. The solvation interaction and ion-dipole interaction were also investigated. The contents of phospholipids in soybean, sunflower, peanut, apricot kernel, filbert and walnut were determined by the recommended method. The results obtained by the nonaqueous capillary electrophoreses were in good agreement with those determined by micellar electrokinetic chromatography.

The roles of protein and lipid in the accumulation and distribution of perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) in plants grown in biosolids-amended soils

“…Plant lipids determined included neutral lipids, phospholipids and fatty acids (Bai et al, 2010;Guo et al, 2005;Rizov and Doulis, 2001). Armitage et al (2012) proposed that phospholipids may dominate the contribution of total lipid to an organism's sorption capacity for PFASs due to their zwitterionic properties, which are similar to those of protein.…”

Nonaqueous capillary electrophoresis for analysis of the ethanol consumption biomarker phosphatidylethanol

“…The separation method presented here was based on the NACE method described by Guo et al [25], using a separation medium based on a 3:2 solution, by volume, of ACN and 2-propanol with ammonium acetate as electrolyte. However some modifications of this separation medium were required.…”

“…A separation medium based on a 3:2 solution, by volume, of ACN and 2-propanol with ammonium acetate as electrolyte as described by Guo et al [25] was tried. Peth however remained nondissolved in this medium.…”

“…Phospholipids have recently been analyzed with nonaqueous CE (NACE) with success [24][25][26]. With this in background, NACE turns out to be an attractive separation method for Peth, being an abnormal phospholipid.…”

Nonaqueous capillary electrophoresis in pharmaceutical analysis

“…For instance, solubility which would have been an issue for analyzing the following compounds in aqueous media, is overcome by performing NACE: sertindole [19], phospholipids [56], tanshinones [67] or steroidal alkaloids [79,81]. A recent communication by Hansen and Sheribah [18] reported the analysis of bromazepam and its related impurities by aqueous CE, MECK, MEEKC and NACE.…”