Non-animal Peptone for Serum Free Cultivation of Recombinant Mammalian and Animal Cells

Abstract

Gene expression by large-scale transfection of mammalian and animal cells is becoming an established technology for the fast production of therapeutic biotech products. However, efforts are still needed to optimize production parameters in order to maximize volumetric productivity while maintaining product quality.Generally, in cell culture 5% to 15% serum is used as supplement in the standard basal media in addition to salts and amino acids for healthy cell growth. To overcome the serum related problems, serum derived and animal derived substances are being used. The serum, serum derived or animal derived substances have some advantages and disadvantages which we have summarized in this review. This article mainly focuses on the replacement of serum, serum derived or animal derived substance with plant derived peptones to fulfill the regulatory requirements.In addition, this article also highlights the methods of peptone selection, peptone screening and some issues related to peptone utilization and their solutions.On the basis of review, we found that the usage of ultrafiltered plant peptone in combination with polyamine could be the best composition for cells growth and reproducible expression of the therapeutic Bio-products. The source or type of peptone (soy peptone, wheat and rice peptone) and polyamines ( cadaverine, putrescine, spermidine, spermine, agmatine and ornithine etc) are cell dependent and can be selected on the basis of their performance with respect to cell growth, expression of protein of interest with consistency in the production process.

Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line

“…could promote growth and cause the observed batch‐to‐batch variation 1,7‐10 . Some causes of the batch‐to‐batch variability in performance were investigated and several publications pointed to the potential impacts of seasonal effect during the soybean cultivation or during the production process of soy hydrolysates 5,6,11,12 . The manufacturing process includes several steps from protein hydrolysis, filtrations steps, pasteurization and drying.…”

“…1,[7][8][9][10] Some causes of the batch-to-batch variability in performance were investigated and several publications pointed to the potential impacts of seasonal effect during the soybean cultivation or during the production process of soy hydrolysates. 5,6,11,12 The manufacturing process includes several steps from protein hydrolysis, filtrations steps, pasteurization and drying. Hartshorn et al were able to correlate cell culture performance with the level of some components and changes in seven parameters of the hydrolysates manufacturing process over eight process steps.…”

Photodesorption of specific organic compounds from titanium dioxide particles in aqueous media

“…A recent study by El Saliby et al [1] involved the photodesorption of dissolved organic carbon using SWW, which is a complex blend of several organic and inorganic compounds. The organic constituents are; beef extract (a mixture of peptides and amino acids, nucleotide fractions, organic acids, minerals and some vitamins [6]), peptone (an enzymatic digest of animal protein consisting of short polymers of amino acids linked by peptide bonds [7]), humic acid (a complex mixture of acids containing carboxyl and phenolated groups that behaves as a dibasic or occasionally a tribasic acid [8]), tannic acid (a polyphenolic compound containing hydroxyl groups that cross link to other compounds), sodium lauryl sulphate (an anionic orthosulfonate consisting of a 12-carbon chain attached to a sulfate group [9]), and gum arabic powder (a mixture of polysaccharides and glycoproteins [10; 11]). The organic compounds in SWW can be classified as polysaccharides (as found in beef extract, arabic acid and arabic gum powder), proteins and amino acids (as found in beef extract and peptone) and depending on the functional group attached, humic acid, tannic acid and sodium lignin sulphonate can be classified as carboxylic and phenolic hydrocarbons where as sodium lauryl sulphate represents an organosulphate compound.…”