Nocardiopsis aegyptia sp. nov., isolated from marine sediment

Abstract

An actinomycete, strain SNG49 T , was isolated from marine sediment of Abu Qir Bay, on the western seashore of Alexandria, Egypt. The bacterium was aerobic and Gram-positive. It produced beige to light-yellow aerial mycelium, brown substrate mycelium and straight to flexuous hyphae, but no specific spore chains. 16S rDNA sequence analysis and chemotaxonomic markers were consistent with classification of strain SNG49T in the genus Nocardiopsis, i.e. mesodiaminopimelic acid; no diagnostic sugars; phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol as polar lipids; menaquinones of the MK-10 series from MK-10(H 0 ) to MK-10(H 8 ); and iso/anteiso-branched and 10-methyl-branched fatty acids, the principal fatty acids being anteiso-17 : 0 and tuberculostearic acid. Nocardiopsis lucentensis and Nocardiopsis alba are the phylogenetic neighbours of strain SNG49 T , respectively showing 98?8 and 98?7 % 16S rRNA gene sequence similarity; however, moderate DNA-DNA reassociation values between these two species and strain SNG49 T (44 and 60 %, respectively) showed that strain SNG49 T could be clearly separated from them. These data, together with distinct physiological traits, led to the conclusion that this isolate represents a novel species within the genus Nocardiopsis, for which the name Nocardiopsis aegyptia is proposed. The type strain is SNG49 T (=DSM 44442 T

Degradation of polyesters by a novel marine Nocardiopsis aegyptia sp. nov.: Application of Plackett-Burman experimental design for the improvement of PHB depolymerase activity

“…T was proposed and the phylogenetic analysis was carried out (Sabry et al, 2004 Polymeric materials. Poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxy-butyrate-co-3-hydroxyvalerate) P(3HB-co-10-20% HV) were obtained as powders from Aldrich (Steinheim, Germany) and ICI (Brillingham, UK), respectively.…”

Nocardiopsis arabia sp. nov., a halotolerant actinomycete isolated from a sand-dune soil

“…At the time of writing, the taxon encompasses 24 recognized species (Li et al, 2006), which form a distinct clade within the evolutionary radiation occupied by members of the family Nocardiopsaceae (Rainey et al, 1996;Cui et al, 2001;Kroppenstedt & Evtushenko, 2006). Nocardiopsis strains are frequently isolated from saline and alkaline soils (Li et al, , 2006Hozzein et al, 2004), but have also been recovered from an alkaline slag dump (Schippers et al, 2002), clinical material (Yassin et al, 1997;Mordarska et al, 1998), indoor environments (Peltola et al, 2001), the atmosphere of a composting facility (Kämpfer et al, 2002) and a marine sediment (Sabry et al, 2004). In the present polyphasic study, an actinomycete isolated from a sand-dune soil was shown to represent a novel species of the genus Nocardiopsis.…”

Salinactinospora qingdaonensis gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond

“…Most strains of the genus Nocardiopsis were isolated from hypersaline soils (M.-G. W.-J. Li et al, , 2006Chen et al, 2008;Yang et al, 2008) and some strains were isolated from marine sediments (Sabry et al, 2004;Kroppenstedt & Evtushenko, 2006;Tian et al, 2009). The four species of the genus Thermobifida were isolated from composts or damp stored hay (Zhang et al, 1998), manure heaps (Kukolya et al, 2002) and a salt mine .…”