Nocardioides gilvus sp. nov., isolated from Namtso Lake

Abstract

A Gram-stain positive, facultatively anaerobic, non-motile and rod to coccoid-shaped bacterium, designated XZ17(T), was isolated from Namtso Lake of Tibet, China. Strain XZ17(T) grew optimally at pH 8.0-9.0, at 30-33 °C and in the presence of 0-1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain XZ17(T) belongs to the genus Nocardioides in the family Nocardioidaceae. Strain XZ17(T) shared pairwise 16S rRNA gene sequence similarities of 97.2, 96.8, 96.5, 96.4 and <96.0 % to Nocardioides solisilvae KCTC 39528(T), Nocardioides daejeonensis JCM 16922(T), Nocardioides jensenii NCIB 9770(T), Nocardioides dubius KCTC 9992(T) and other Nocardioides species, respectively. It contained MK-8 (H4) as the predominant menaquinone and C17:1 ω8c, C15:0, C17:0 and C18:1 ω9c as the major fatty acid. The strain had cell wall peptidoglycan based on LL-2,6-diaminopimelic acid. The polar lipids of strain XZ17(T) comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids and two unidentified lipids. The DNA G+C content of strain XZ17(T) was 68.9 mol%. Based on distinctive phenotypic characteristics, phylogenetic analysis and chemotaxonomic data, it can be concluded that strain XZ17(T) represents a novel species within the genus Nocardioides, for which the name Nocardioides gilvus sp. nov. is proposed. The type strain is strain XZ17(T) (=KCTC 39561(T) = MCCC 1H00114(T)).

Nocardioides humilatus sp. nov., isolated from farmland soil in the Republic of Korea

“…The genus Nocardioides , which belongs to the family Nocardioidaceae in the order Actinomycetales of the class Actinobacteria was proposed by Prauser [1] for Gram-positive, non-acid-fast, aerobic and mesophilic nocardioform actinomycetes, developing mycelia that fragment into irregular rod- to coccus-like elements [2]. At the time of writing (January 2020), the genus Nocardioides comprises 111 recognized species (www.bacterio.net/nocardioides.html), which have been recovered from a wide variety of habitats such as desert soil [3], plants [4], animals [5], sea- and fresh water [6], saltern [7], volcanic ash [8], indoor air [9], glacier cryoconite [10] and polluted environments [11]. The chemotaxonomic features are a quinone system with the major menaquinone MK-8(H 4 ) and cell-wall peptidoglycan containing ll -diaminopimelic acid.…”

Nocardioides jishulii sp. nov.,isolated from faeces of Tibetan gazelle (Procapra picticaudata)

“…net/ identify) [18]. Strains dk3136 T and dk3543 were identical (99.8 %) in their 16S rRNA gene sequences and showed a high similarity to Nocardioides solisilvae Ka25 T (97.9 %) [19], Nocardioides gilvus XZ17 T (97.7 %) [11], Nocardioides houyundeii 78 T (97.6 %) [13], Nocardioides daphniae D287 T (97.5 %) [12], Nocardioides yefusunii HY056 T (97.5 %) [20] and other species of the genus Nocardioides (<97.4 %), indicating that they are very likely to represent a novel species of the genus Nocardioides [21]. Fig.…”

“…net/ nocardioides. html), which have been isolated from various sources, such as soil [2][3][4][5], seawater [6], desert [7], plants [8][9][10], lake [11] and animals [12]. In our previous studies, several novel species of the order Actinomycetales have been isolated from wild animals (Tibetan antelope, vultures and plateau pika) from the Qinghai-Tibet Plateau, including Nocardioides houyundeii [13], Actinomyces gaoshouyii [14], Actinomyces tangfeifanii [15] and Actinomyces vulturis [16].…”

“…Phylogenetic trees were reconstructed using the neighbor-joining (NJ) [23], maximum-likelihood (ML) [24] and maximum-parsimony (MP) [25] algorithms with Kimura's two-parameter model and the tree topology reliability was evaluated by bootstrap analysis based on 1000 resampled datasets [26]. Strains dk3136 T and dk3543 fell into the genus Nocardioides and clustered with N. daphniae D287 T and N. gilvus XZ17 T [11] in the NJ phylogenetic tree [ Fig. 1, an extended version of the tree is available in Fig.…”

“…¶DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PL, phospholipids; GL, glycolipids; L, lipid; PE, phosphatidylethanolamine; PI, phosphatidylinositol. **Data from Zhang et al [11]. Table 3.…”