Nocardioides humilatus sp. nov., isolated from farmland soil in the Republic of Korea

Lee, Jong Min; Jin, Chun-Zhi; Kang, Min‐Kyoung; Park, So Hee; Park, Dong-Jin; Kim, Dong-Gyun; Kim, Chang‐Jin

doi:10.1099/ijsem.0.004928

Cited by 8 publications

(2 citation statements)

References 46 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After inoculation of the cells, observation continued for four weeks. The development of spores was examined as described previously using SEM in the aerial mycelium on the tested media after cells had grown on R2A for 30 days [ 18 , 19 ]. Anaerobic growth was examined on the R2A agar medium using a GasPak EZ Anaerobe Pouch System (Beckton Dickinson and Company, Sparks, MD, USA) at 28 °C.…”

Section: Methodsmentioning

confidence: 99%

Genomic Insight into Shimazuella Soli Sp. Nov. Isolated from Soil and Its Putative Novel Class II Lasso Peptide

et al. 2022

Self Cite

View full text Add to dashboard Cite

The strain designated as AN120528T was isolated from farmland soil in South Korea. This strain grows well on R2A medium at 28 °C. The cells are an off-white colour and have no hyphae. The phylogenetic analysis indicated that the strain is a member of the genus Shimazuella with a 98.11% similarity to Shimazuella alba KC615T and a 97.05% similarity to S. kribbensis KCTC 9933T, respectively. The strain AN120528T shares common chemotaxonomic features with the other two type strains in the genus. It has MK-9 (H4) and MK-10 (H4) as its predominant menaquinones. The major fatty acids are iso-C14:0, iso-C15:0, anteiso-C15:0 and iso-C16:0. Diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), lipids (L), and aminolipids (AL) were identified as the major cellular polar lipids. Analysis of the peptidoglycan showed the presence of meso-diaminopimelic acid. Whole-genome sequencing revealed that the genome of the strain is approximately 3.3 Mbp in size. The strain showed a 77.5% average nucleotide identity (ANI) with S. alba KC615T. The genomic DNA (gDNA) G + C content is 39.0%. Based on polyphasic taxonomy analysis, it is proposed that this strain, AN120528T, represents a novel species in the genus Shimazuella, designated as Shimazuella soli sp. nov. The type stain is AN120528T (=KCTC 39810T = DSM 103571T). Furthermore, shimazuellin I, a new 15-amino-acid peptide, was discovered in the AN120528T through genome mining; it has the features of a lasso peptide, containing eight amino acids (-G-Q-G-G-S-N-N-D-) that form a macrolactam ring and seven amino acids (-D-G-W-Y-H-S-K-) that form a tail.

show abstract

Section: Methodsmentioning

confidence: 99%

Genomic Insight into Shimazuella Soli Sp. Nov. Isolated from Soil and Its Putative Novel Class II Lasso Peptide

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The complete genome sequencing was performed using the de novo MGI platform (BGI, China), according to previous studies [25,26]. The reads for sequencing were quality trimmed to the Q 30 confidence level and assembled with CLC Assembly Cell 5.1.1 (Qiagen Inc, USA).…”

Section: Genome Sequencing and Genomic Analysismentioning

confidence: 99%

Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food

Park

Choi

et al. 2023

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

We investigated the probiotic characteristics and anti-obesity effect of Lactiplantibacillus plantarum MGEL20154, a strain that possesses excellent intestinal adhesion and viability. The in vitro properties, e.g., gastrointestinal (GI) resistance, adhesion, and enzyme activity, demonstrated that MGEL20154 is a potential probiotic candidate. Oral administration of MGEL20154 to diet-induced obese C57BL/6J mice for 8 weeks resulted in a feed efficacy decrease by 44.7% compared to that of the high-fat diet (HFD) group. The reduction rate of weight gain was about 48.5% in the HFD+MGEL20154 group compared to that of the HFD group after 8 weeks, and the epididymal fat pad was also reduced in size by 25.2%. In addition, the upregulation of the zo-1, pparα, and erk2, and downregulation of the nf-κb and glut2 genes in Caco-2 cells by MGEL20154 were observed. Therefore, we propose that the anti-obesity effect of the strain is exerted by inhibiting carbohydrate absorption and regulating gene expression in the intestine.

show abstract

Description and genomic characterization of Nocardioides bruguierae sp. nov., isolated from Bruguiera gymnorhiza

Chen

Zheng

et al. 2023

Systematic and Applied Microbiology

View full text Add to dashboard Cite

Nocardioides humilatus sp. nov., isolated from farmland soil in the Republic of Korea

Cited by 8 publications

References 46 publications

Genomic Insight into Shimazuella Soli Sp. Nov. Isolated from Soil and Its Putative Novel Class II Lasso Peptide

Genomic Insight into Shimazuella Soli Sp. Nov. Isolated from Soil and Its Putative Novel Class II Lasso Peptide

Anti-Obesity Potential through Regulation of Carbohydrate Uptake and Gene Expression in Intestinal Epithelial Cells by the Probiotic Lactiplantibacillus plantarum MGEL20154 from Fermented Food

Description and genomic characterization of Nocardioides bruguierae sp. nov., isolated from Bruguiera gymnorhiza

Contact Info

Product

Resources

About