No evidence for a major effect of three common polymorphisms of the GPx1, MnSOD, and CAT genes on PCOS susceptibility

Salahshoor, Mohammad Reza; Sohrabi, Maryam; Jalili, Faramarz; Jalili, Parnian; Rezavand, Negin; Haghnazari, Lida; Jalili, Cyrus

doi:10.1002/jcb.27564

Cited by 8 publications

(8 citation statements)

References 49 publications

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“…Our results suggest that the L allele of GPx1 P198L polymorphism is a genetic risk factor for PCOS in Chinese women. However, our study was unable to prove that the CAT C-262T genetic polymorphism was associated with the risk of PCOS in Chinese women, which was consistent with the report by Salahshoor et al (28).…”

Section: Discussionsupporting

confidence: 70%

“…Because GPx1 and CAT are important intracellular antioxidant enzymes and play an important role in maintaining the dynamic balance of oxidative stress interacting with SOD2 (26, 27), we hypothesized that the functional SNPs, the P198L in GPx1 gene and the C-262T in CAT gene, may be associated with PCOS. Salahshoor et al (28) reported that the GPx1 P198L and the CAT C-262T SNPs were not associated with the risk of PCOS in the Kurdish population, but it remains unknown whether there are any relationships between these 2 gene polymorphisms and PCOS in Chinese women. In the present study, we investigated the relationships between GPx1 P198L and CAT C-262T variants and the risk of PCOS, evaluated the effects of the genotypes on clinical, hormonal, metabolic and oxidative stress parameters, and analyzed the associations of the genotype combinations of GPx1 P198L and SOD2 A16V with PCOS in well-characterized Chinese women using relatively large sample sizes.…”

Section: Introductionmentioning

confidence: 99%

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Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women

Sun

Liu

et al. 2019

Front. Endocrinol.

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Background: Oxidative stress plays an important role in the pathogenesis of polycystic ovary syndrome (PCOS). Glutathione peroxidase 1 (GPx1) and catalase (CAT) are the major intracellular antioxidant enzymes that can detoxify hydrogen peroxide into water, preventing cellular injury from reactive oxygen species. The aim of the present study was to investigate the association of GPx1 P198L (Pro198Leu, C559T, rs1050450) and CAT C-262T (rs1001179) genetic polymorphisms with the risk of PCOS and evaluate the effects of the genotypes on clinical, hormonal, metabolic and oxidative stress parameters in Chinese women.Methods: This is a case-control study of 654 patients with PCOS and 535 controls. The GPx1 P198L, CAT C-262T, and superoxide dismutase 2 (SOD2) A16V genotypes were determined by polymerase chain reaction amplification and restriction analysis. Clinical, hormonal, metabolic and oxidative stress parameters were also analyzed.Results: The frequencies of the PL + LL genotype (14.1 vs. 8.4%) and L allele (7.3 vs. 4.4%) of GPx1 P198L polymorphism were significantly higher in patients with PCOS than in control subjects. Genotype (PL + LL) remained a significant predictor for PCOS in prognostic models including age, body mass index (BMI), insulin resistance index, total cholesterol, triglycerides, high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol as covariates (OR = 2.105, 95%CI: 1.330–3.331, P = 0.001). Patients carrying the L allele had relatively high average ovarian volume, waist circumference, and malondialdehyde levels (P < 0.07) compared with patients with the PP genotype. We also demonstrated that the subjects with both GPx1 L and SOD2 A alleles further increase the risk of PCOS compared with the individuals carrying the PP/VV genotype after adjusting for age and BMI (OR = 5.774, 95%CI: 2.243–14.863, P < 0.001). However, no significant differences were observed in the frequencies of the CAT C-262T genotypes and alleles between PCOS and control groups.Conclusions: The GPx1 P198L, but not CAT C-262T, genetic polymorphism is associated with the risk of PCOS in Chinese women.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women

Sun

Liu

et al. 2019

Front. Endocrinol.

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“…This disorder, which is considered a chronic systemic disorder, is often related to insulin resistance, hyperandrogenemia, chronic inflammation, and oxidative stress. Although the mechanisms of PCOS pathogenesis are not fully identified, some studies propose a role for oxidative stress (OS) in the disorder (5,6). Oxidative stress results from an imbalance in the production and removal of reactive oxygen and nitrogen species (ROS and RNS, respectively).…”

Section: Introductionmentioning

confidence: 99%

Assessment of GSTO1 (A140D) and GSTO2 (N142D) Gene Polymorphisms in Iranian Women with Polycystic Ovarian Syndrome

et al. 2020

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Background: Glutathione S-transferases (GSTs) protect cells from oxidative stress (OS). In humans, the GST omega class contains two expressed genes, GSTO1 and GSTO2. Because OS is involved in the pathogenesis of polycystic ovary syndrome (PCOS), the aim of this study was to investigate the relationship between GSTO1 A140D (rs4925) and GSTO2 N142D (rs156697) polymorphisms in PCOS patients. Methods: 175 PCOS patients and 161 healthy controls were selected among women in Kermanshah province, Iran. GSTO1 and GSTO2 were genotyped using allele-specific PCR (AS-PCR) and PCR-RFLP, respectively. Results: For GSTO1, the DD genotype and the D allele led to 2.17-(P= 0.02) and 1.5-fold (P= 0.01) increases, respectively, in the odds ratios for PCOS. No significant difference was found between control and patient groups for the GSTO2 N142D genotype or allele frequency. GSTO1 and GSTO2 genotype interaction analysis showed that individuals with the GSTO1 AD or DD genotypes and the GSTO2 NN or DN genotypes had a 1.53-fold (P= 0.007) increase in PCOS risk over GSTO1 AA and GSTO2 DD individuals. Conclusions: The GSTO1 A140D polymorphism is a risk factor for PCOS.

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“…GPX is another antioxidant metabolizing enzyme which detoxifies the hydrogen peroxide via converting it to water in presence of glutathione. There are eight isoforms for GPX (GPx1–8), of which GPx‐1 is known as the main isoform . CATs are known as the first line of defense against hydrogen peroxide by converting it to water and oxygen.…”

Section: Introductionmentioning

confidence: 99%

“…There are eight isoforms for GPX (GPx1-8), of which GPx-1 is known as the main isoform. 13 CATs are known as the first line of defense against hydrogen peroxide by converting it to water and oxygen. The hemoenzyme is a powerful antioxidant enzyme because of its high turnover rate.…”

Section: Introductionmentioning

confidence: 99%

The effects of the genetic polymorphisms of antioxidant enzymes on susceptibility to papillary thyroid carcinoma

et al. 2020

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Several lines of evidences have indicated that inflammation play an important role in the carcinogenesis. During the inflammatory processes, free radical species are produced from oxidative stress. In normal conditions, enzymatic and nonenzymatic antioxidants remove these products. Manganese superoxide dismutase (MnSOD), glutathione peroxidase‐1 (GPx‐1), and catalase (CAT) are three important enzymes. Therefore, this study aimed to evaluate the effects of MnSOD (SOD2), GPX‐1, and CAT genetic polymorphisms on papillary thyroid carcinoma (PTC) susceptibility. A total of 134 patients with PTC and 151 healthy controls were recruited to participate in this study. All samples were genotyped for SOD2 rs4880, GPX1 1050450, and CAT rs7943316 polymorphisms by polymerase chain reaction‐restriction fragment length polymorphism method. The frequencies of the rs1050450, rs4880, and rs7943316 alleles and genotypes were not different between PTC patients and controls. However, the TC genotype of SOD2 rs4880 polymorphism was significantly higher in males compared to that in females in PTC patients (odds ratio [OR], 3.9 [95% CI, 1.5–11], p = .007). The rs4880 polymorphism was also associated with higher stages (III‐IV) of PTC in dominant model. No significant correlation was found between GPX1‐rs1050450 and CAT‐rs7943316 polymorphisms and demographic, clinical, and pathological features of the disease. The SOD2 rs4880CT genotype was more frequent in males with PTC and patients with higher stages (III–IV) of disease (OR, 2.9 [95% CI, 1.1–7.7], p = .04). However, no significant association was found between GPX1‐rs1050450 and CAT‐rs7943316 variants and PTC or its demographic, clinical, and pathological features.

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No evidence for a major effect of three common polymorphisms of the GPx1, MnSOD, and CAT genes on PCOS susceptibility

Abstract: GPx1 (rs1050450), MnSOD (rs4880), and CAT (rs1001179) variants might not be a risk factor for PCOS.

Cited by 8 publications

References 49 publications

Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women

Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women

Assessment of GSTO1 (A140D) and GSTO2 (N142D) Gene Polymorphisms in Iranian Women with Polycystic Ovarian Syndrome

The effects of the genetic polymorphisms of antioxidant enzymes on susceptibility to papillary thyroid carcinoma

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