Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women

Sun, Yuan; Li, Suiyan; Liu, Hongwei; Gong, Yan; Bai, Huai; Huang, Wei; Liu, Qingqing; Guan, Linbo; Fan, Ping

doi:10.3389/fendo.2019.00771

Cited by 26 publications

(15 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The catalase protects the genome of mouse oocytes from oxidative damage during meiotic maturation and its activity in ovarian granulosa and theca cells are known to increase during ovarian development and luteinization [22]. In addition, the cooperative effect of gpx and catalase has been investigated in other studies [45][46][47]. For instance, the upregulation of gpx supported catalase activity in the presence of hydrogen peroxide, thereby inhibiting ROS-induced cell death [46].…”

Section: Discussionmentioning

confidence: 99%

High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

Kang

et al. 2020

Animals

View full text Add to dashboard Cite

Advanced maternal age (AMA) has become prevalent globally. With aging, weakened antioxidant defense causes loss of normal function in the ovary and uterus due to oxidative stress. Here, we aimed to improve embryo development in AMA mice by intravenous injection (IV) of human adipose stem cell conditioned medium (ASC-CM) at various frequencies and intervals as an antioxidant intervention. Four- and six-month-old female ICR (Institute of Cancer Research) mice were randomly divided into groups IV treated with human ASC-CM under different conditions, and in vitro and in vivo embryo development were evaluated. Consequently, compared to the control group, blastocyst formation rate of parthenotes was significantly promoted in 4-month-old mice and the mean number of implanted fetuses after natural mating was significantly increased by approximately two-fold in 6-month-old mice. Through gene analysis, the anti-apoptotic and anti-oxidative effects of human ASC-CMs were confirmed in the ovaries and uterus of pregnant mice at both ages. In particular, ovarian expression of gpx1 and catalase drastically increased in 6-month-old mice. Furthermore, the levels of gpx1 and catalase were further increased, with a high frequency of injection regardless of age. Thus, we demonstrated for the first time the anti-oxidative effect of human ASC-CM administration against ovarian aging and the optimal injection condition.

show abstract

Section: Discussionmentioning

confidence: 99%

High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

Kang

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…Serum estradiol (E 2 ), total testosterone (TT), luteinizing hormone (LH), follicle‐stimulating hormone (FSH), sex hormone‐binding globulin (SHBG), triglyceride (TG), total cholesterol (TC), HDL‐cholesterol (HDL‐C), LDL‐cholesterol (LDL‐C), apolipoprotein (apo)A1, apoB, total oxidant status (TOS), malondialdehyde (MDA) and total antioxidant capacity (T‐AOC) levels, plasma insulin and glucose concentrations, homeostatic model assessment of insulin resistance (HOMA‐IR), free androgen index (FAI) and oxidative stress index (OSI) were determined or assessed as described previously 4,22,26 …”

Section: Methodsmentioning

confidence: 99%

“…Oxidative stress plays an important role in the pathogenesis of PCOS, 4,6,22,23 and patients with PCOS have increased MPO levels 24 . However, it remains unknown whether the MPO G‐463A and CYBA C242T SNPs are related to PCOS.…”

Section: Introductionmentioning

confidence: 99%

Myeloperoxidase andCYBAgenetic variants in polycystic ovary syndrome

Liu

et al. 2020

Eur J Clin Investigation

Self Cite

View full text Add to dashboard Cite

Background Oxidative stress plays a pivotal role in the pathogenesis of polycystic ovary syndrome (PCOS). Genetic variations in myeloperoxidase (MPO; G‐463A) and NADPH oxidase p22phox subunit (CYBA; C242T) cause inter‐individual variability in enzyme activities. Here, we investigated the associations between MPO activity and the MPO G‐463A and CYBA C242T polymorphisms in Chinese women with PCOS. Methods This case‐control study included 1003 patients with PCOS and 810 controls. The G‐463A and C242T polymorphisms were detected by polymerase chain reaction and restriction analysis, and clinical, hormonal, metabolic and oxidative stress parameters and MPO activity were analysed. Results The frequencies of the GA + AA genotype and A allele frequency of the MPO G‐463A polymorphism were significantly higher in the PCOS group than in the control group. Logistic regression analysis showed that the MPO‐463A allele is a risk factor for PCOS (OR = 1.261, 95% CI: 1.042‐1.526, P = .017). Patients with the AA genotype tended to have higher plasma MPO activity than those with the GG genotype. No statistical significance was found in the genotype and allele frequencies of the CYBA C242T polymorphism between the PCOS and control groups. However, we demonstrated that the coexistence of the MPO A allele (GA + AA genotypes) and the CYBA CC genotype was associated with an increased risk of PCOS when compared with the wild‐type GG/CC genotypes (OR = 1.302, 95% CI: 1.030‐1.646, P = .027). Conclusion The MPO G‐463A variant, but not CYBA C242T variant, is associated with a risk of PCOS in Chinese women.

show abstract

“…Individuals with diabetes show mutations and alterations in genes involved in the antioxidant pathway that influence the progression of the disease 47 . Studies conducted among Chinese women with PCOS have reported an association between this syndrome and the P198L polymorphism of GPX1 48 . However, a study in Iran found no association between the risk of PCOS and GPX1 (rs1050450) or SOD2 (rs4880) polymorphisms 49 …”

Section: Metabolism and Pcosmentioning

confidence: 99%

Genetic polymorphisms associated with polycystic ovary syndrome among Iranian women

Jamshidi

Pour

Bahadoram

et al. 2020

Intl J Gynecology & Obste

View full text Add to dashboard Cite

Polycystic ovary syndrome (PCOS) involves abnormalities in ovarian, reproductive, and metabolic systems. Genetic polymorphisms associated with individual differences and variations might be related to complex disorders with unknown causes, including PCOS. Several leading genetic markers with known cellular functions have been identified among Iranian women presenting with PCOS. In particular, the existing evidence shows a significant relationship between PCOS and the following genetic polymorphisms: rs2275913 (interleukin‐17A), rs9927163 (interleukin‐32), Pro12Ala (peroxisome proliferator‐activated receptor‐γ), rs17173608 (chemerin), rs2236242 (vaspin), ApaI (vitamin D receptor), and rs7895833 (sirtuin 1). In addition, a higher risk of PCOS is associated with the rs2910164 (microRNA 146a), rs2241766 (adiponectin), –34 T/C (cytochrome 17), and rs1800682 (Fas) polymorphisms. Furthermore, protective effects against PCOS have been reported for the A4223C polymorphism of adenosine deaminase 1. Overall, the available data indicate that Iranian women with PCOS have a higher prevalence of polymorphisms in inflammation‐ and metabolism‐related genes, but not in insulin‐related genes. More extensive studies are needed to identify the ethnicity‐related genetic associations in PCOS.

show abstract

Association of GPx1 P198L and CAT C-262T Genetic Variations With Polycystic Ovary Syndrome in Chinese Women

Cited by 26 publications

References 51 publications

High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

Myeloperoxidase andCYBAgenetic variants in polycystic ovary syndrome

Genetic polymorphisms associated with polycystic ovary syndrome among Iranian women

Contact Info

Product

Resources

About