1998
DOI: 10.1002/pro.5560070410
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NMR determination of the global structure of the 113Cd derivative of desulforedoxin: Investigation of the hydrogen bonding pattern at the metal center

Abstract: Desulforedoxin (Dx) is a simple homodimeric protein isolated from Desulfovibrio gigas (Dg) containing a distorted rubredoxin‐like center with one iron coordinated by four cysteinyl residues (7.9 kDa with 36 amino acids per monomer). In order to probe the geometry and the H‐bonding at the active site of Dx, the protein was reconstituted with 113Cd and the solution structure determined using 2D NMR methods. The structure of this derivative was initially compared with the NMR solution structure of the Zn form (Go… Show more

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Cited by 22 publications
(10 citation statements)
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“…The values obtained for 2h J HN-Cd coupling constants fall in the range 0.3-1.1 Hz (Table 2). These values are of similar magnitude to ones reported previously for rubredoxin and desulfodextrin [23,24,37]. A comparison of the coupling constants with the corresponding NHAEAEAES distances indicates that there is no direct correlation between the magnitude of 2h J HN-Cd and NHAEAEAES bond length.…”
Section: Hydrogen-bonding Interactionssupporting
confidence: 84%
“…The values obtained for 2h J HN-Cd coupling constants fall in the range 0.3-1.1 Hz (Table 2). These values are of similar magnitude to ones reported previously for rubredoxin and desulfodextrin [23,24,37]. A comparison of the coupling constants with the corresponding NHAEAEAES distances indicates that there is no direct correlation between the magnitude of 2h J HN-Cd and NHAEAEAES bond length.…”
Section: Hydrogen-bonding Interactionssupporting
confidence: 84%
“…18 For amide groups in such hydrogen bonds, 48 chemical shift changes presumably result from small changes in the hydrogen bond lengths caused by the increase in metal-sulphur bond length. 49,50 Smaller chemical shift changes may reflect minor structural rearrangements.…”
Section: Metal Exchange Kineticsmentioning
confidence: 99%
“…Nevertheless, the nature of the metal binding in the case of GSH remains subject to debate, as cadmium has been proposed to link to amide [15], carboxylates of both glycine [13,14] and glutamate residues [13,14,16] or the amine NH 2 lone pair [11,13,16]. 113 Cd NMR [12] was used to characterize this interaction, but without success, in contrast to many 113 Cd–protein experiments [17–19]. Simulation of theoretical chemical shifts [20,21] or EXAFS experiments [22] were also performed to analyse the cadmium(II) sphere of co‐ordination.…”
Section: Introductionmentioning
confidence: 99%
“…Cd NMR [12] was used to characterize this interaction, but without success, in contrast to many 113 Cd-protein experiments [17][18][19]. Simulation of theoretical chemical shifts [20,21] or EXAFS experiments [22] were also performed to analyse the cadmium(II) sphere of co-ordination.…”
mentioning
confidence: 99%