NMR-based metabolomics Reveals Alterations of Electro-acupuncture Stimulations on Chronic Atrophic Gastritis Rats

Xu, Jingjing; Zheng, Xiaodong; Cheng, Kian Kai; Chang, Xiao-Rong; Shen, Guiping; Liu, Mi; Wang, Yadong; Shen, Jiayun; Zhang, Yuan; He, Qi-da; Dong, Jiyang; Yang, Zongbao

doi:10.1038/srep45580

Cited by 45 publications

(50 citation statements)

References 52 publications

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“…The rats in control group were freely accessed to water clean water and standard rat diet. According to our previous study 25 , all of rats except ones in control group were subjected to CAG modeling including compulsive sporting, irregular fasting as well as mixture of 30% alcohol and 2% sodium salicylate poured down the throats for 12 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…S13 ). According to previous studies 25 , for rats in the electro-acupuncture treatment group, Liangmen (ST 21) and Zusanli (ST 36) were treated with electro-acupuncture (a 30-minute session per day) for 2 weeks after CAG modeling. While for CAG rats with electro-acupuncture on non-acupoints, the corresponding non-acupoints located 5 mm away from each of the two acupoints were treated with electro-acupuncture as mentioned above (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…In our preliminary study, 1 H NMR-based metabolomics coupled molecular biology is used to analyze biological samples (stomach, cortex urine, serum and medulla) in rats with CAG and after electro-acupuncture treatment. And it was found that electro-acupuncture treatment could relieve CAG by regulating membrane catabolism, function of neurotransmitter in brain and gut microbiota metabolism 24 , 25 . In this study, the difference of therapeutic mechanism between electro-acupuncture and moxibustion on rats with CAG is investigated by 1 H NMR-based metabolomics combined with pathological evaluation and molecular biological assay.…”

Section: Introductionmentioning

confidence: 99%

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Comparative metabolomics study on therapeutic mechanism of electro-acupuncture and moxibustion on rats with chronic atrophic gastritis (CAG)

Liu

Chen

Chang

et al. 2017

Sci Rep

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Some studies have proved that both acupuncture and moxibustion are very effective for the treatment of CAG. However, little is known about therapeutic mechanism of electro-acupuncture and moxibustion on CAG as well as the difference between them. On the other hand, metabolomics is a ‘top-down’ approach to understand metabolic changes of organisms caused by disease or interventions in holistic context, which consists with the holistic thinking of electro-acupuncture and moxibustion treatment. In this study, the difference of therapeutic mechanism between electro-acupuncture and moxibustion on CAG rats was investigated by a 1H NMR-based metabolomics analysis of multiple biological samples (serum, stomach, cerebral cortex and medulla) coupled with pathological examination and molecular biological assay. For all sample types, both electro-acupuncture and moxibustion intervention showed beneficial effects by restoring many CAG-induced metabolic changes involved in membrane metabolism, energy metabolism and function of neurotransmitters. Notably, the moxibustion played an important role in CAG treatment mainly by regulating energy metabolism in serum, while main acting site of electro-acupuncture treatment was nervous system in stomach and brain. These findings are helpful to facilitate the therapeutic mechanism elucidating of electro-acupuncture and moxibustion on CAG rats. Metabolomics is promising in mechanisms study for traditional Chinese medicine (TCM).

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative metabolomics study on therapeutic mechanism of electro-acupuncture and moxibustion on rats with chronic atrophic gastritis (CAG)

Liu

Chen

Chang

et al. 2017

Sci Rep

Self Cite

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“…It demonstrates that acupuncture may have a potential disadvantage on improving the appetite of depression model rats in comparison with western medicine. Nevertheless, some researches showed that stimulations to acupoints of the stomach meridian such as Sibai (ST 2), Liangmen (ST 21), and Zusanli (ST 36) were effective in treating functional dyspepsia 98 , in which poor appetite is one of the core symptoms. In our analysis, Baihui (GV20) and Yintang (GV29) were most frequently used, while acupoints of stomach are scarcely used.…”

Section: Discussionmentioning

confidence: 99%

Acupuncture for behavioral changes of experimental depressive disorder: a systematic review and meta-analysis

Kou

Chen

et al. 2017

Sci Rep

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Acupuncture is considered to be a promising alternative therapy for depression. Nevertheless, up to now, it remains controversial on the effects exerted by acupuncture on behavioral changes in depression models. Consequently, it’s necessary to develop a systematic review and meta-analysis to assess the effect of acupuncture for model rats of depression. Ultimately, 90 studies involving 1861 models were identified. Behavioral indicators including the number of crossings (NC) and the number of rearings (NR) in open field test (OFT), the capacity of sucrose intake (CSI) and the rate of sucrose intake (RSI) in sucrose intake test (SIT), final weight (FW) and gain weight (GW) were employed as main outcomes in depression model rats. The pooled results showed that acupuncture had not less effect than western medicine on improving NC, NR, FW, GW, RSI (P > 0.05). However, the CSI improvement was poorer compared with west medicine (P < 0.05). In conclusion, acupuncture has not less effect on behavior changes than western medicine, including improving NC, NR, RSI, FW and GW in depression models.

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“…Some studies had proved that the points of the stomach meridian had a good therapeutic effect on gastric mucosal injury [10,16,17]. Meanwhile, our previous studies proved that GU could be treated effectively by acupuncture at Liangmen (ST 21) and Zusanli (ST 36), which belong to the stomach meridian [18][19][20][21]. erefore, "Liangmen" (ST21), "Zusanli" (ST36), "Riyue" (GB24), and "Yanglingquan" (GB34) were selected for treatment by moxibustion (Supplementary Figure S1).…”

Section: Moxibustion Treatmentmentioning

confidence: 99%

Effect of Moxibustion on Intestinal Microbiome in Acute Gastric Ulcer Rats

Huang

Zhang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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In Traditional Chinese Medicine (TCM), moxibustion had been used for thousands of years. Many clinical case reports and scientific studies had proved that moxibustion had a good effect in treating acute gastric ulcer (AGU). Some studies had shown that the relative content and species of bacteria in the intestinal would be changed when gastric mucosal injury happened. However, there was little research on the effect of intestinal microbiome with AGU rats that were treating by moxibustion. This study is aimed at analyzing the effect of fecal microbiome in rats with AGU by the 16S rDNA sequencing technology. Male SD rats were established by orally feeding once with 70% ethanol at 4 ml/kg except the control group, then treated by moxibustion in the stomach meridian group (“Liangmen,” “Zusanli”) and the gallbladder meridian group (“Riyue,” “Yanglingquan”) for 5 days. The 16S rDNA sequencing technology analysis of feces combined with histopathological methods and molecular biological detection methods was used to evaluate the therapeutic mechanism of moxibustion on AGU. AGU brought cause changes in the number and species of intestinal bacteria. Moxibustion on stomach meridian group could reduce the area of gastric mucosal injury and regulate the relative content of GAS and EGF. Moreover, moxibustion on the stomach meridian group could increase the relative content and species of beneficial bacteria in the intestine of rats with AGU. The relative abundance of intestinal probiotics was significantly upregulated in Alphaproteobacteria, Actinomycetales, and Bacillales. In addition, moxibustion might promote the repair of gastric mucosal injury by increasing the number and species of beneficial bacteria in the intestine.

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NMR-based metabolomics Reveals Alterations of Electro-acupuncture Stimulations on Chronic Atrophic Gastritis Rats

Cited by 45 publications

References 52 publications

Comparative metabolomics study on therapeutic mechanism of electro-acupuncture and moxibustion on rats with chronic atrophic gastritis (CAG)

Comparative metabolomics study on therapeutic mechanism of electro-acupuncture and moxibustion on rats with chronic atrophic gastritis (CAG)

Acupuncture for behavioral changes of experimental depressive disorder: a systematic review and meta-analysis

Effect of Moxibustion on Intestinal Microbiome in Acute Gastric Ulcer Rats

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