Effect of Moxibustion on Intestinal Microbiome in Acute Gastric Ulcer Rats

Abstract

In Traditional Chinese Medicine (TCM), moxibustion had been used for thousands of years. Many clinical case reports and scientific studies had proved that moxibustion had a good effect in treating acute gastric ulcer (AGU). Some studies had shown that the relative content and species of bacteria in the intestinal would be changed when gastric mucosal injury happened. However, there was little research on the effect of intestinal microbiome with AGU rats that were treating by moxibustion. This study is aimed at analyzing the effect of fecal microbiome in rats with AGU by the 16S rDNA sequencing technology. Male SD rats were established by orally feeding once with 70% ethanol at 4 ml/kg except the control group, then treated by moxibustion in the stomach meridian group (“Liangmen,” “Zusanli”) and the gallbladder meridian group (“Riyue,” “Yanglingquan”) for 5 days. The 16S rDNA sequencing technology analysis of feces combined with histopathological methods and molecular biological detection methods was used to evaluate the therapeutic mechanism of moxibustion on AGU. AGU brought cause changes in the number and species of intestinal bacteria. Moxibustion on stomach meridian group could reduce the area of gastric mucosal injury and regulate the relative content of GAS and EGF. Moreover, moxibustion on the stomach meridian group could increase the relative content and species of beneficial bacteria in the intestine of rats with AGU. The relative abundance of intestinal probiotics was significantly upregulated in Alphaproteobacteria, Actinomycetales, and Bacillales. In addition, moxibustion might promote the repair of gastric mucosal injury by increasing the number and species of beneficial bacteria in the intestine.

Molecular targets of Yangyin Fuzheng Jiedu Prescription in the treatment of hepatocellular carcinoma based on network pharmacology analysis

“…Prior evidence has suggested roles for epidermal growth factor receptor (EGFR), estrogen receptor 1 (ESR1), and prostaglandin-endoperoxide synthase 2 (PTGS2) in the molecular mechanisms of Astragalus membranaceus (Huáng Qí, a flowering plant in the family Fabaceae used in TCM) and applied animal models to investigate its mechanism of action [ 13 ]. Moxibustion, another TCM method, has been reported to exercise its beneficial effects through modulating the production of epidermal growth factor (EGF) and the activity of AKT serine/threonine kinase 1 (AKT1) [ 14 ]. Moreover, biochemical investigated have identified mitogen-activated protein kinase 1 (MAPK1) and jun proto-oncogene (JUN) as the key targets underlying the efficacy of the Chinese patent medicine Zhixiong Capsule [ 15 ].…”

Effects of Electroacupuncture on the Gut Microbiome in Cisplatin-Induced Premature Ovarian Failure Mice

“…There are a large number of microbiomes in the human intestine, including probiotics, pathogenic bacteria, and conditioned pathogens [ 23 ]. The gut microbiome is considered to be closely related to human health [ 24 ].…”

Effect of Electro-Acupuncture on Gut Microbiome in Cisplatin-Induced Premature Ovarian Failure Mice

“…There are a large number of microbiomes in human intestine, including probiotics, pathogenic bacteria and conditioned pathogen [22]. Gut microbiome is considered to be closely related to human health [23].…”