Nitric oxide (NO) counteracts cadmium induced cytotoxic processes mediated by reactive oxygen species (ROS) in Brassica juncea: cross-talk between ROS, NO and antioxidant responses

Verma, Kavita; Mehta, S.; Shekhawat, G. S.

doi:10.1007/s10534-013-9608-4

Cited by 120 publications

(58 citation statements)

References 68 publications

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“…In wheat roots, exogenous NO ameliorates Cd toxicity by attenuating Cd-induced ROS generation (Singh et al 2008). Similar results were observed in mustard, where NO counteracts Cdinduced cytotoxic processes mediated by ROS (Verma et al 2013). In both wheat and mustard, authors have reported that apart from the downregulation of ROS production NO decreased also stress response, including antioxidant enzymes.…”

Section: Introductionsupporting

confidence: 57%

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

et al. 2015

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Background and Aims The objective of this study was to analyse the role of nitric oxide (NO) in relation to reactive oxygen species (ROS) production and root growth inhibition in the early response of barley root tip to cadmium. Methods We used cell-permeable NO-binding dye DAF-2DA for the NO localization and cellimpermeable dye DAR-4M for the quantification of NO in barley root apex. Results A considerable increase in NO production was detected in the transition zone of root tips already 30 min after the immersion of barley roots into 15 μM Cd. This Cd-induced NO generation further increased during the recovery period and reached its maximum level 2 h after the short-term treatment. At this stage an enhanced Cd-induced NO generation was observed in the meristematic, transition and in the beginning of elongation zone in comparison with control roots. Inhibitor of NO synthase activity markedly reduced root growth in control seedlings and had a synergistic growth inhibitory effect with Cd. On the contrary, this Cdinduced root growth inhibition was markedly alleviated by the NO donor. Exposure of roots to Cd markedly increased the production of ROS in the proximal elongation zone of root. While NO did not affect ROS generation, inhibition of its synthesis slightly decreased ROS generation in both control and Cd-treated roots. Conclusions Our results revealed that enhanced NO production is a very early response of barley root to Cd stress and it is involved in the regulation of root growth mitigating Cd-induced root growth inhibition.

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Section: Introductionsupporting

confidence: 57%

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

et al. 2015

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“…First of all, NO could directly scavenge the overproduced ROS in cells. Secondly, NO might function as a signaling molecule to modulate antioxidative gene expression (Verma et al 2013). However, accumulating evidence shows that NO is a double edged sword in plants.…”

Section: Introductionmentioning

confidence: 99%

Effects of nitric oxide on alleviating cadmium stress in Typha angustifolia

et al. 2015

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Nitric oxide (NO) is a key signal molecule that is involved in plant response to various abiotic stresses. The present study was conducted to investigate the effects of NO on Typha angustifolia which has been subjected to cadmium stress. Our results showed that sodium nitroprusside (SNP, a NO donor) treatment could significantly mitigate both the Cd (CdCl 2 Á2.5H 2 O)-induced increase of malondialdehyde content in the root and relative electrolyte leakage in the root and leaf. NO demonstrated a reduced or counteractive effect on the Cd-induced increase in the activities of some typical antioxidant enzymes. The Cdinduced changes on the contents of non-protein thiol and phytochelatins in the root could also be reversed by NO. The protected effect of NO is likely achieved through trapping Cd in the root and decreasing Cd accumulation in the leaf and sheath. Furthermore, NO enhanced the allocation of Cd in the cell wall and reduced the distribution of Cd in the soluble fraction of the root and leaf. Differential biosynthesis of ascorbic acid content in some tissues might also be responsible for the protected effect of NO. This study concludes that NO counteracts Cd toxicity strongly in T. angustifolia via regulating antioxidant metabolism and enhancing Cd accumulation in the cell wall of the root.

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“…Enzymatic and non-enzymatic antioxidants exhibit two classic patterns of endogenous protective mechanisms to prevent oxidative stress (35). Antioxidant enzymes, including SOD and GSH-Px, may clear accumulated OFR and associated metabolites to maintain homeostasis and attenuate the effects of ROS, thus protecting cellular structures from destruction and prevent the onset of fatigue (36). In the present study, TM treated female and male mice exhibited increased serum, hepatic and muscular SOD and GSH-Px, and decreased MDA and ROS levels to different degrees depending on the concentration of TM administered.…”

Section: Tm (G/kg) Tm (G/kg) ----------------------------------------mentioning

confidence: 99%

The anti‑fatigue activities of Tuber melanosporum in a mouse model

Jiang

Chu

et al. 2018

Exp Ther Med

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Abstract. Tuber melanosporum (TM) is an edible fungus that exhibits antioxidant and anti-tumor activity via its unique bioactive metabolites. The present study analyzed the anti-fatigue effects of TM using a BALB/c mouse model. The anti-fatigue properties of TM were evaluated by assessing the endurance of mice by performing forced swimming, rotary rod and running tests. Following 2 weeks TM treatment, hepatic and muscular ATP, and glycogen levels were increased in mice subjected to 30 min swimming, compared with controls. Similarly, levels of serum lactic acid and lactic dehydrogenase were decreased in the same group, compared with the control. Additionally, TM treatment reduced reactive oxygen species and malondialdehyde levels, and increased superoxide dismutase and glutathione peroxidase levels in the muscle, liver and/or serum. The effect of TM on hormone levels was also investigated in the present study, as different efficacies of TM were observed in male and female mice. TM treatment increased serum levels of progesterone, estradiol and testosterone in female and male mice, whereas a decrease in serum luteinizing hormone levels was only observed in females. A decrease in serum follicle-stimulating hormone levels was identified in females, whereas an increase was observed in males. The current study demonstrated that the anti-fatigue effects of TM occur via the regulation of oxidative stress, energy metabolism and hormone levels.

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Nitric oxide (NO) counteracts cadmium induced cytotoxic processes mediated by reactive oxygen species (ROS) in Brassica juncea: cross-talk between ROS, NO and antioxidant responses

Cited by 120 publications

References 68 publications

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

Effects of nitric oxide on alleviating cadmium stress in Typha angustifolia

The anti‑fatigue activities of Tuber melanosporum in a mouse model

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