Nitric oxide is a neurotransmitter in the chloride secretory response to serotonin in rat colon

Stoner, Michael C.; Scherr, Adam M; Lee, Judy A.; Wolfe, Luke; Kellum, John M.

doi:10.1067/msy.2000.107608

Cited by 27 publications

(22 citation statements)

References 19 publications

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“…Radionuclide 36 Cl as HCl was obtained from PerkinElmer Life Sciences. Caco-2 cells were obtained from the ATCC (Manassas, VA).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have suggested the involvement of 5-HT4 receptors in the stimulation of cAMP (36). Therefore, it was of interest to examine the role of PKA in 5-HT-mediated effects on Cl Ϫ /OH Ϫ exchange activity in Caco-2 cells.…”

Section: Possible Role Of Pkamentioning

confidence: 99%

“…However, studies of Eklund et al (35) and Donowitz et al (31) have shown that cAMP plays no role in mediating the secretory effects of 5-HT in cat and rabbit intestine. Recent studies (36) point out the role of nitric oxide in mediating the effects of 5-HT-induced Cl Ϫ secretion in the rat colon. The current studies were therefore undertaken to examine the following: (i) the possible regulation of Cl Ϫ /OH Ϫ exchange activity in Caco-2 cells (a well established model for the human intestinal transport studies) by serotonin; (ii) to characterize the 5-HT receptor subtype(s) involved; and (iii) the signal transduction pathways involved in this process.…”

Section: -Htmentioning

confidence: 99%

“…This period was chosen because it falls within the linear range of Cl Ϫ uptake in this system (38,39). For 36 Cl Ϫ uptake studies, the uptake buffer was the mannitol buffer containing 1.4 Ci of 36 Cl Ϫ (2.9 mM) of hydrochloric acid (specific activity-17.12 mCi/g) Ϯ 0.3 mM DIDS. The uptake was terminated by removing the buffer and washing the cells rapidly two times with 1 ml of ice-cold phosphatebuffered saline, pH 7.2 (PBS).…”

Section: CLmentioning

confidence: 99%

“…Cl Ϫ /OH Ϫ exchange activity was measured as DIDS-sensitive (300 M) 36 Cl uptake at 5 min. Values represent mean Ϯ S.E.…”

Section: Table III Effect Of Pkc Inhibitors On 5-ht-induced Inhibitiomentioning

confidence: 99%

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Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Saksena

Gill

Tyagi

et al. 2005

Journal of Biological Chemistry

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The gastrointestinal tract is an important source of the endogenous amine, serotonin, mainly stored in the mucosal enterochromaffin cells and in the enteric nervous system (1). 5-HT1 is continuously released from the enterochromaffin cells in the intestinal mucosa into the portal circulation and the gut lumen in response to a variety of luminal stimuli such as change in pH or osmolarity and mechanical and chemical stimuli (2). 5-HT is an important neurotransmitter and intercellular messenger to modulate gastrointestinal functions (3). 5-HT has been shown to alter gastrointestinal motility (4), alter gastric acid secretion, and enhance intestinal secretions (1, 5). Most of the previous studies regarding the effects of serotonin on ion transport were mainly based on the electrophysiological data including short circuit current measurements and unidirectional fluxes. These studies showed that 5-HT stimulated electrogenic Cl Ϫ secretion and inhibited Na ϩ and Cl Ϫ absorption in the rat colon (6), jejunum (7), ileum (8), and guinea pig ileum (9). 5-HT has also been shown to inhibit the NaClabsorptive process in the rabbit ileum and gall bladder (10) but has no effect in rabbit and guinea pig colon (10, 11). Moreover, Sundaram et al. (12) have demonstrated that 5-HT inhibits Cl Ϫ /HCO 3 Ϫ exchange activity in villus cells and stimulates HCO 3 Ϫ secretion in crypt cells of the rabbit ileum. In contrast, 5-HT appears to have no significant effect on coupled NaCl absorption in the human jejunum (13).5-HT exerts its effects by binding to 5-HT receptors, which are distributed on the neuronal, muscular, and epithelial structures (14). Based on pharmacological studies and molecular cloning (15), the existence of at least seven 5-HT receptors (5-HT1-7) subdivided into 14 subtypes has been discovered. With the exception of 5-HT5 and 5-HT6, 5-HT1 A , 5-HT1 P , 5-HT2, 5-HT3, and 5-HT4 have been reported to be expressed in the gut (16). 5-HT1, 5-HT2, and 5-HT4 receptors are coupled via guanine nucleotide binding (G) proteins to their effectors, whereas 5-HT3 receptor is a ligand-gated ion channel permeable to anions and cations (17).

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“…Radionuclide 36 Cl as HCl was obtained from PerkinElmer Life Sciences. Caco-2 cells were obtained from the ATCC (Manassas, VA).…”

Section: Methodsmentioning

confidence: 99%

Section: Possible Role Of Pkamentioning

confidence: 99%

Section: -Htmentioning

confidence: 99%

Section: CLmentioning

confidence: 99%

“…Cl Ϫ /OH Ϫ exchange activity was measured as DIDS-sensitive (300 M) 36 Cl uptake at 5 min. Values represent mean Ϯ S.E.…”

Section: Table III Effect Of Pkc Inhibitors On 5-ht-induced Inhibitiomentioning

confidence: 99%

See 3 more Smart Citations

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Saksena

Gill

Tyagi

et al. 2005

Journal of Biological Chemistry

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Germ-Free Mice Model for Studying Host–Microbial Interactions

Bhattarai

Kashyap

2016

Methods in Molecular Biology

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Germ-free (GF) mice are a relevant model system to study host-microbial interactions in health and disease. In this chapter, we underscore the importance of using GF mice model to study host-microbial interactions in obesity, immune development and gastrointestinal physiology by reviewing current literature. Furthermore, we also provide a brief protocol on how to setup a gnotobiotic facility in order to properly maintain and assess GF status in mice colonies.

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Effects of nitric oxide in 5-hydroxytryptamine-, cholera toxin-, enterotoxigenic Escherichia coli- and Salmonella Typhimurium-induced secretion in the porcine small intestine

Grøndahl¹,

Unmack²,

Ragnarsdóttir³

et al. 2005

Comparative Biochemistry and Physiology Part A: Molecular &

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Nitric oxide is a neurotransmitter in the chloride secretory response to serotonin in rat colon

Cited by 27 publications

References 19 publications

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Germ-Free Mice Model for Studying Host–Microbial Interactions

Effects of nitric oxide in 5-hydroxytryptamine-, cholera toxin-, enterotoxigenic Escherichia coli- and Salmonella Typhimurium-induced secretion in the porcine small intestine

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