“…In fact, the effect of NO on oocyte maturation can be stimulatory or inhibitory depending on its concentration (Bu et al, 2003(Bu et al, , 2004Bilodeau-Goeseels, 2007). Positive effects of SNAP administration during IVM were observed in the mouse (Goud et al, 2005), rat , and pig (Petr et al, 2005(Petr et al, , 2006. In our IVM system, the addition of 100 µM SNAP slightly increased blastocyst and hatching rates of oocytes derived from Lo ovaries to a percentage comparable with that observed in oocytes isolated from Hi ovaries; conversely, it negatively affected the hatching rate of oocytes from Hi ovaries.…”