The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Petr, Jaroslav; Eva, Carola; Tůmová, Lenka; Ješeta, Michal

doi:10.17221/2323-cjas

Cited by 4 publications

(7 citation statements)

References 156 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elevated cytosolic free Ca 2þ level might be due to increased NO and cGMP levels during meiotic resumption from diplotene as well as M-II arrest. These data corroborates with previous observations that the increased intracellular Ca 2þ level trigger EM-II arrest through NO-cGMP-mediated pathway (Petr et al, 2005(Petr et al, , 2006(Petr et al, , 2007. Our results together with previous findings suggest that the reduction of NO and cGMP levels trigger meiotic resumption from diplotene arrest, while there increase associates with EM-II arrest.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes

Prasad

Tiwari

Tripathi

et al. 2015

Cell Biology International

View full text Add to dashboard Cite

The present study was aimed to find out changes in signal molecules and maturation promoting factor (MPF) levels during meiotic cell cycle progression from diplotene and metaphase-II (M-II) arrest, a period during which oocyte achieves meiotic competency. Data suggest that high levels of adenosine 3'-5'-cyclic monophosphate (cAMP), guanosine 3'-5'-cyclic monophosphate (cGMP), and nitric oxide (NO) are associated with diplotene arrest, while reduction in their levels correlates with reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increased intracellular NO, calcium (Ca(2+) ) as well as hydrogen peroxide (H2 O2 ) levels correlate with decreased cAMP, Thr-161 phosphorylated cyclin-dependent kinase-1 (Cdk1) as well as cyclin B1 levels. The decreased Thr-161 phosphorylated Cdk1 and cyclin B1 level reduce MPF level leading to exit from M-II arrest in oocytes cultured in vitro. These data suggest that the decrease of cAMP level and increase of cytosolic free Ca(2+) as well as H2 O2 levels associate with the reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increase of NO, cGMP, Ca(2+) as well as H2 O2 levels are associated with reduced MPF and spontaneous exit from M-II arrest in rat oocytes cultured in vitro.

show abstract

Section: Discussionsupporting

confidence: 93%

“…The NO and cGMP are other signal molecules that regulate meiotic cell cycle in oocytes (Petr et al, 2005(Petr et al, , 2006(Petr et al, , 2007. Previous studies suggest that reduction of intraoocyte NO level leads to meiotic resumption from diplotene arrest (Chaube et al, 2008;Tripathi et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes

Prasad

Tiwari

Tripathi

et al. 2015

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…However, they are unable to exit from the first metaphase to reach the second meiotic metaphase stage, and to complete meiotic maturation. Full meiotic competence is acquired in pig oocytes reaching an internal diameter of 120 µm (Szybek, 1972;Sorensen and Wassarman, 1976;Motlík and Fulka, 1986;Petr et al, 1994Petr et al, , 1999Petr et al, , 2007aBrevini et al, 2007).…”

mentioning

confidence: 99%

Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence

Eva¹,

Sedmı́ková²,

Petr³

et al. 2009

CZECH J ANIM SCI

Self Cite

View full text Add to dashboard Cite

The intensive development of biotechnologies such as in vitro embryo production and cloning by nuclear transfer requires a great number of oocytes with fully-developed meiotic competence.During in vitro culture these oocytes are able to reach the stage of second meiotic metaphase and complete their meiotic maturation. However, the number of these oocytes in the ovary is limited. ABSTRACT: Reproduction biotechnologies depend on the use of fully meiotically competent oocytes. Growing oocytes without full meiotic competence are an interesting potential source due to their quantity, but the mechanisms regulating the processes of acquisition of meiotic competence have not been clarified to date. Nitric oxide synthase (NOS) and its product, nitric oxide (NO), may possibly play a role. Understanding the precise NO regulatory mechanism is therefore important for the development of in vitro growth methods. The objective of this work was to detect changes in the expression of NOS isoforms and their mRNA expression and changes in the intracellular localization of separate NOS isoforms during the growth period of the porcine oocyte, and also to determine whether these changes are related to the process of meiotic competence acquisition. mRNA for all NOS isoforms was already detected in oocytes at the beginning of their growth and was present in them until they completed their growth period. mRNA for iNOS and eNOS was also observed in granulosa and cumulus cells from these oocytes. But nNOS mRNA was not demonstrated in these types of cells. Pig oocytes and their surrounding cells contained all NOS proteins. Their amounts increased and localization changed with the acquisition of meiotic competence. nNOS was localized mainly in the cortex in meiotically incompetent oocytes, while meiotically competent oocytes contained nNOS in the nucleus as well. iNOS protein was distributed in the cytoplasm and nucleus in all oocytes, and meiotically incompetent oocytes contained iNOS in the nucleolus as well. eNOS protein was distributed in oocytes in the form of fine granules with a strong fluorescence signal. Protein was concentrated in the nuclear area in meiotically incompetent oocytes and also in the periphery in oocytes with partially and fully-developed meiotic competence. All these findings indicate that NOS isoforms may significantly influence the acquisition of meiotic competence in porcine oocytes.

show abstract

“…Changes in intracellular signalling by free calcium ions seem to be important for oocyte activation using NO-donor, and these changes are at least partially triggered by the cGMP-dependent signalling cascade (Kuo et al, 2000;Petr et al, 2005bPetr et al, , 2006Petr et al, , 2007a. These effects on calcium signalling could mimic the intracellular oscillation of free calcium ions which is induced by phospholipase C-zeta after fertilization of the oocyte by sperm (Jones et al, 1998;Parrington et al, 2002;Fujimoto et al, 2004;Konig et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…There was no exocytosis of cortical granules, and the development of parthenogenetic embryos did not proceed beyond the 4-cell stage (Petr et al, 2005a). Pig oocytes are activated by donors of nitric oxide only after continuous treatment lasting more than 10 hours (Petr et al, 2005a(Petr et al, , 2007a and the development of activated oocytes is seriously compromised (Petr et al, 2005a(Petr et al, , 2007a.…”

mentioning

confidence: 99%

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Krejcova¹,

Petr²,

Krejčová³

et al. 2009

CZECH J ANIM SCI

Self Cite

View full text Add to dashboard Cite

Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments during which oocytes were repeatedly exposed to 2mM SNAP for a short time (10, 20 or 30 minutes) were more efficient with regard to the activation rate, even when the total exposure time did not exceed 4 hours. Parthenogenetic development was very limited after continuous treatment with 2mM SNAP. A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to the morula stage 0 vs. 18%; development to the blastocyst 0 vs. 7%; P < 0.05). However, this developmental rate was significantly lower (P < 0.05) than the development induced by conventional activation treatment with calcium ionophore (development to the morula stage, 23%; development to the blastocyst stage, 18%). When we combined pulsatile SNAP-treatment with the effect of protein kinase inhibitor 6-dimethyl aminopurine (6-DMAP) (2mM 6-DMAP for 2 hours) or with the inhibitor of protein synthesis cycloheximide (CHX) (10 µM CHX for 2 hours), we observed a significant increase (P < 0.05) in the activation rate when compared to the respective pulsatile SNAP-treatment without 6-DMAP or CHX (63 vs. 78% of activated oocytes for 6-DMAP; 63 vs. 83% of activated oocytes for CHX). However, the development of parthenogenetic embryos was not enhanced when the pulsatile SNAP-treatment was combined with 6-DMAP or with CHX.

show abstract

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Cited by 4 publications

References 156 publications

Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes

Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes

Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Contact Info

Product

Resources

About