The endothelial nitric oxide synthase/nitric oxide system is involved in the defective quality of bovine oocytes from low mid-antral follicle count ovaries1

Tessaro, Irene; Luciano, A.M.; Franciosi, Federica; Lodde, Valentina; Corbani, D.; Modina, S.

doi:10.2527/jas.2010-3714

Cited by 38 publications

(28 citation statements)

References 51 publications

(54 reference statements)

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“…The Hi ovaries were used as the age-matched control group. In both categories, the presence or absence of a corpus luteum was not taken into account, as stated previously (Gandolfi et al 1997;Modina et al 2007;Tessaro et al 2011). It has been demonstrated that the AFC is not affected by the stage of the oestrous cycle (Cushman et al 2009).…”

Section: Methodsmentioning

confidence: 95%

“…The ovaries from each cow were isolated and classified into one of two categories, as described previously (Modina et al 2007;Tessaro et al 2011). Briefly, ovaries were classified as Lo (¼ low efficiency) ovaries when they were found to contain ,10 follicles between 2 and 6 mm in diameter and no follicles $10 mm or as Hi (¼ high efficiency) ovaries when they contained .10 follicles between 2 and 6 mm in diameter (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…In previous studies, we demonstrated that the oocytes of 4-to 8-year-old dairy cows that had ovaries with fewer than 10 mid-antral follicles (2-6 mm in diameter) and no follicles $10 mm in diameter ('Lo' ovaries) exhibited reduced developmental capability compared with age-matched controls in which both ovaries had .10 mid-antral follicles ('Hi' ovaries; Modina et al 2007). Moreover, defective endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) signalling was found in Lo ovaries in addition to reduced follicle vasculature, which could adversely affect oocyte quality and early embryonic in vitro development (Tessaro et al 2011). Therefore, the aim of the present study was to assess whether in a population of culled dairy cows of reproductive age those cows with Lo ovaries had morphological and endocrine markers commonly associated with premature ovarian aging that could, in turn, lead to a premature decline in fertility.…”

Section: Introductionmentioning

confidence: 99%

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Reductions in the number of mid-sized antral follicles are associated with markers of premature ovarian senescence in dairy cows

Modina

Tessaro

Lodde

et al. 2014

Reprod. Fertil. Dev.

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Abstract. High-producing dairy cows are subfertile; however, the mechanisms responsible for the decreased fertility are unknown. The aim of the present study was to test the hypothesis that culled dairy cows (4-8 years old) characterised by 'Lo' ovaries (i.e. those with ,10 mid-antral follicles) are affected by premature ovarian senescence. Cows in which both ovaries were 'Lo' ovaries represented 5% of the total population analysed, and exhibited reduced ovarian size (P , 0.001) and increased perifollicular stroma (P , 0.05) compared with age-matched controls (i.e. cows in which both ovaries had .10 mid-antral follicles; 'Hi' ovaries). The total number of follicles, including healthy and atretic primordial, primary, secondary and small antral follicles, was lower in Lo ovaries (P , 0.01). Interestingly, the primordial follicle population in Lo ovaries was lower (P , 0.05) than in the control. Finally, the follicular fluid of mid-antral follicles from Lo ovaries had reduced oestradiol and anti-Müllerian hormone levels (P , 0.05), but increased progesterone concentrations (P , 0.05). Together, these data account for the reduced fertility of cows with Lo ovaries and are in agreement with previous observations that oocytes isolated from Lo ovaries have reduced embryonic developmental competence. Cows with a specific Lo ovary condition may represent a suitable model to address the causes of low fertility in high-yielding dairy cows, as well as the condition of premature ovarian aging in single-ovulating species.

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Section: Methodsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reductions in the number of mid-sized antral follicles are associated with markers of premature ovarian senescence in dairy cows

Modina

Tessaro

Lodde

et al. 2014

Reprod. Fertil. Dev.

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“…2009; Mossa et al, 2012) and in vitro (Tessaro et al, 2011) that could be due to decreased oocyte quality or differences in serum progesterone concentrations .…”

Section: Discussionmentioning

confidence: 99%

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility12

Cushman

Miles

Rempel

et al. 2013

Journal of Animal Science

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ABSTRACT:A proposed functional polymorphism in the ionotropic glutamate receptor AMPA1 (GRIA1) has been reported to influence antral follicle numbers and fertility in cows. Repeat breeder cows that fail to produce a calf in multiple seasons have been reported to have reduced numbers of small (1 to 3 mm) antral follicles in their ovaries. Therefore, we tested the hypothesis that this GRIA1 polymorphism was affecting antral follicle numbers in repeat breeder cows. Repeat breeder cows (n = 64) and control cows (n = 72) that had always produced a calf were housed in a dry lot and observed twice daily for behavioral estrus. Blood samples were collected, and cows were genotyped for this GRIA1 polymorphism and for a polymorphism in the GnRH receptor (GnRHR) that was proposed to influence age at puberty. On d 3 to 8 after estrus cows were slaughtered, and reproductive organs were collected to determine antral follicle count, ovary size, and uterine horn diameter. Repeat breeder cows were older at first calving than control cows (P = 0.006). The length (P = 0.03) and height (P = 0.02) of the ovary contralateral to the corpus luteum (CL) were greater in control cows than repeat breeder cows. The endometrial diameter in the horn ipsilateral to the CL was greater in the control cows than the repeat breeder cows. Repeat breeder cows had fewer small (1 to 5 mm) antral follicles than control cows (P = 0.003); however, there was no association between GRIA1 genotype and antral follicle number. The GnRHR polymorphism was associated with age at first calving because cows that were homozygous for the C allele had a greater age at first calving than heterozygous cows or cows that were homozygous for the T allele (P = 0.01). In the granulosa cells from small (1 to 5 mm) antral follicles, mRNA abundances of 2 markers of oocyte quality, anti-Müllerian hormone and pentraxin 3, did not differ between fertility groups (P ≥ 0.12). We conclude that this GRIA1 polymorphism exists in beef cows but that it does not influence antral follicle numbers. The association between GnRHR genotype and age at first calving is likely not causal as this polymorphism is not functional. The utility of this polymorphism as a genetic marker for early conception in heifers will require further validation. Screening postpartum cows by ultrasonography to determine antral follicle numbers may aid in making culling decisions.

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“…The modulatory role of NO on the follicular angiogenic process appears still controversial at the present time, as positive effects of NO on follicle development and selection related to angiogenic events have been instead demonstrated in bovine (GrazulBilska et al 2007, Tessaro et al 2011, horse (Pinto et al 2003), and sheep (Seekallu et al 2010). …”

Section: Role Of No In the Control Of Follicular Angiogenesismentioning

confidence: 99%

Nitric oxide in follicle development and oocyte competence

Basini

Grasselli

2015

Reproduction

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Apart from its well-known role in regulating endothelial function, in mammals, nitric oxide (NO) is an important signaling molecule involved in many processes, regulating different biological functions. It has been demonstrated that NO plays a role in the physiology of the reproductive system, where it acts in controlling the activity of reproductive organs in both sexes. In the female of several animal species, experimental data suggest the presence of an intraovarian NO-generating system, which could be involved in the control of follicular development. The role of NO in regulating follicular atresia by apoptosis is still controversial, as a dual action depending mostly on its concentration has been documented. NO also displays positive effects on follicle development and selection related to angiogenic events and it could also play a modulatory role in steroidogenesis in ovarian cells. Both in monovulatory and poliovulatory species, the increase in PGE 2 production induced by NO via a stimulatory effect on COX-2 activity appears to be a common ovulatory mechanism. Considerable evidence also exists to support an involvement of the NO/NO synthase system in the control of meiotic maturation of cumulus-oocyte complexes.Reproduction (2015) 150 R1-R9

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The endothelial nitric oxide synthase/nitric oxide system is involved in the defective quality of bovine oocytes from low mid-antral follicle count ovaries1

Cited by 38 publications

References 51 publications

Reductions in the number of mid-sized antral follicles are associated with markers of premature ovarian senescence in dairy cows

Reductions in the number of mid-sized antral follicles are associated with markers of premature ovarian senescence in dairy cows

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility12

Nitric oxide in follicle development and oocyte competence

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