Nitrate reductase as a possible predictive test of crop yield

Johnson, Christopher; Whittington, W. J.; Blackwood, G. C.

doi:10.1038/262133a0

Cited by 58 publications

(16 citation statements)

References 7 publications

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“…Lavres et al (2010) observed that the increase in nitrate reductase activity in diagnostic leaves was strongly influenced by N and K supplies in the nutrient solution in the first growing period of Mombaça guineagrass and was affected only by N concentration in the second growth period. Johnson et al (1976), Wiedenfeld et al (2009), andLavres et al (2010) proposed using the activity of nitrate reductase as an index of plant N status, and Fernandes et al (1978) observed that nitrate reductase activity in Brachiaria sp. was correlated positively (r = 0.97 * * ) with N concentrations in leaves and sheaths.…”

Section: Resultsmentioning

confidence: 98%

“…Nitrogen is absorbed mainly as nitrate (NO 3 − ) or ammonium (NH 4 + ) (Johnson et al, 1976;Lavres et al, 2010), which are used in producing a broad array of organic compounds essential to plant metabolism, including nucleic acids, chlorophyll, proteins, and amino acids (Taiz and Zeiger, 2006;Marschner, 2012). In contrast, plant uptake of K involves the absorption of a monovalent ion (K + ) that undergoes no incorporation into organic compounds (Zhang et al, 2011;Marschner, 2012).…”

Section: Introductionmentioning

confidence: 98%

“…Assays for nitrate reductase activity (At-RNA) and chlorophyll meter readings (SPAD), which measures chlorophyll concentrations, have been used to assess plant N status for increasing forage production (Johnson et al, 1976), but SPAD measurements of chlorophyll concentrations are more convenient because they are nondestructive (Ruiz-Espinoza et al, 2010) and can be related to dry matter production, At-RNA, and plant N content (Lavres and Monteiro, 2006;Lavres et al, 2010;Yildirim et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Marandu Palisadegrass Mineral Nutrition and Production Related to Nitrogen and Potassium Supply

Megda

Monteiro

2014

Journal of Plant Nutrition

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2 Nitrogen (N) and potassium (K) fertilization play a key role in forage crops and can significantly increase yields of 'Marandu' palisadegrass [Brachiaria brizantha (Hochst. exA. Rich.) Stapf.], one of the most important forage crops in Brazil. This study aimed to identify the concentrations of total N and K, nitrate (NO 3 − ), and ammonium (NH 4 + ), chlorophyll meter readings (SPAD), and nitrate reductase activity (At-RNA) required to maximize yield. Plants were grown in quartz substrate and treated with nutrient solutions that ranged from 2 to 33 mmol L −1 for N and 0.5 to 11 mmol L −1 for K. Dry matter production and At-RNA increased with increasing N and K supplies. SPAD readings correlated strongly with N leaf concentration and dry matter production and can be used to assess the N status of this species. The supply of N and K in the fertilization promoted high yield and adequate N and K concentration for plant metabolism.

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Marandu Palisadegrass Mineral Nutrition and Production Related to Nitrogen and Potassium Supply

Megda

Monteiro

2014

Journal of Plant Nutrition

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“…Peuke and Tischner (1991) have demonstrated that the former method more *Corresponding author; e-mail sumitrachanda@yahoo.com accurately reflects a plants status than does an in-vitro assay, in which the enzyme is removed from its natural physiological environment and transferred to optimized conditions. In-vivo assays have been successful in agricultural studies (Blondel and Blanc, 1975) for estimating the optimized level and appropriate time of application for nitrogen on field crops (Chanda et al, 1987), for predicting plant productivity (Johnson et al, 1976), and for screening crop cultivars with high yield potential (Hageman et al, 1976). It has also been employed in research on the source of reducing energy for nitrate reduction (Mann et al, 1979), and for estimating nitrate pools (Ferrari et al, 1973;Saroop et al, 1999).…”

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confidence: 98%

Factors affecting nitrate reductase activity in some monocot and dicot species

Chanda

2003

J. Plant Biol.

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Activity of nitrate reductase (NR), the first enzyme in the nitrate-assimilation pathway, was estimated in the cotyledons of the sunflower (Helianthus annuus) using a standardized in-vivo method. Seedlings were grown in the light on a nitrate medium. Various factors that affect NR activity were optimized, including the molarity and pH of the reaction buffer, nitrate concentration, and use of a surfactant. We also determined whether NADH was required for nitrate reduction. The surfactant propanol (2%) gave the best results, and no NADH supplement was necessary; In a separate study, we compared the effect of various culturing components on in-vivo NR activity among monocot and dicot species, and found that Triton X-100 was the best surfactant for monocots whereas dicots performed better with n-propanol. Monocot species also required additional NADH as an external energy source. Moreover, specific purification procedures were needed to enhance NR activity in dicotyledons. Finally, we also assessed the efficacy of in-vivo versus in-vitro procedures for assaying monocots versus dicots.

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“…(1976), Boyat et Robin (1977), ont 6tabli un critbre de s6lection dans I'am6-lioration des plantes ) partir de cette relation ANR-teneur en prot6ines du grain. Par exemple, Johnson et al (1976) Montaut (1983) sur I'orge, alors que Peterson et al (1963) en ont d6finies trois sur le mais. …”

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Influence De La Fertilisation Azotée Sur L’activité Nitrate Réductase Et La Teneur en Protéines De Trois Variétés D’avoine

Sauvesty¹,

Gendron²

1987

Can. J. Plant Sci.

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The effect of two application modes of nitrogen fertilization on leaf nitrate reductase activity, Ieaf nitrate content and grain and straw protein content was studied in three oat cultivars: Lamar, Cascade and Cabot. The enzymatic activity shows the same biological rhythm for the three cultivars, no matter when the nitrogen was applied; the activity was high at the coleoptile stage, reached a maximum at tillering and then decreased until heading. However, Lamar presented a higher maximum activity at tiliering when nitrogen fertilizer was applied at sowing. Ammonium nitrate applied twice during growth resulted for all cultivars in a longer period of higher activity and at the same time a decrease of its amplitude. In leaves, the nitrate availability at the site of reduction was different according to t"ertilization mode: maximum nitrate content Can. J. Plant

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Nitrate reductase as a possible predictive test of crop yield

Cited by 58 publications

References 7 publications

Marandu Palisadegrass Mineral Nutrition and Production Related to Nitrogen and Potassium Supply

Marandu Palisadegrass Mineral Nutrition and Production Related to Nitrogen and Potassium Supply

Factors affecting nitrate reductase activity in some monocot and dicot species

Influence De La Fertilisation Azotée Sur L’activité Nitrate Réductase Et La Teneur en Protéines De Trois Variétés D’avoine

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